Human cord blood leukocyte metallothionein-2A MRNA expression is associated with primiparaous and multiparous childbirth
Metallothionein (MT), a metal and stress-induced protein, provides transport and storage of metal ions. The current study analyzed the influence of human cord blood leukocyte [Zn] and gravida on leukocyte MT-2A mRNA expression. Samples (n= 47) were collected at the labor rooms of a Government hospit...
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| Format: | Proceeding Paper |
| Language: | English |
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2013
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| Online Access: | http://irep.iium.edu.my/35414/ http://irep.iium.edu.my/35414/1/MSBMB38_Doc_for_APAR2.pdf |
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| author | Rahman, Mohammad Tariqur Youssof, Ayman Lee Abu Kassim , Noor Lide Rashid, Siti Aishah De Ley, Marc |
| author_facet | Rahman, Mohammad Tariqur Youssof, Ayman Lee Abu Kassim , Noor Lide Rashid, Siti Aishah De Ley, Marc |
| author_sort | Rahman, Mohammad Tariqur |
| building | IIUM Repository |
| collection | Online Access |
| description | Metallothionein (MT), a metal and stress-induced protein, provides transport and storage of metal ions. The current study analyzed the influence of human cord blood leukocyte [Zn] and gravida on leukocyte MT-2A mRNA expression. Samples (n= 47) were collected at the labor rooms of a Government hospital in Kuantan, Malaysia. [Zn] in plasma, erythrocytes, and leukocytes were measured by AAS (λ=213. 9 nm). MT-2A isoform specific mRNA was quantified by real-time PCR using total leukocyte RNA. Quantification of MT-2A gene transcription was normalized to the housekeeping gene, β-actin. Significant correlations were found between plasma [Zn] and erythrocyte [Zn] (r = .304; p< .05) and between leukocyte [Zn] and plasma [Zn] (r = .295; p< .05). Leukocyte [Zn] exhibited a significant correlation with MT-2A mRNA level (r = .765; p< .001). Higher levels of MT-2A mRNA was found in primiparous mothers (n= 16; M= 1.108, SD= 1.273) compared to multiparous mothers (n= 30; M= 0.581, SD= 0.711). Path analysis showed that leukocyte [Zn] has a greater influence (regression weight .84; p= .000) on MT-2A mRNA expression compared to gravida (regression weight -.18; p= .021). Finally it is concluded that, in addition to circulatory Zn, gravida contributes to the MT-2A mRNA expression.
1, NOOR LIDE 2, 3, MARC DE LEY4, MOHAMMAD TARIQUR 1
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| first_indexed | 2025-11-14T15:43:24Z |
| format | Proceeding Paper |
| id | iium-35414 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:43:24Z |
| publishDate | 2013 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-354142014-03-06T03:25:59Z http://irep.iium.edu.my/35414/ Human cord blood leukocyte metallothionein-2A MRNA expression is associated with primiparaous and multiparous childbirth Rahman, Mohammad Tariqur Youssof, Ayman Lee Abu Kassim , Noor Lide Rashid, Siti Aishah De Ley, Marc QH301 Biology Metallothionein (MT), a metal and stress-induced protein, provides transport and storage of metal ions. The current study analyzed the influence of human cord blood leukocyte [Zn] and gravida on leukocyte MT-2A mRNA expression. Samples (n= 47) were collected at the labor rooms of a Government hospital in Kuantan, Malaysia. [Zn] in plasma, erythrocytes, and leukocytes were measured by AAS (λ=213. 9 nm). MT-2A isoform specific mRNA was quantified by real-time PCR using total leukocyte RNA. Quantification of MT-2A gene transcription was normalized to the housekeeping gene, β-actin. Significant correlations were found between plasma [Zn] and erythrocyte [Zn] (r = .304; p< .05) and between leukocyte [Zn] and plasma [Zn] (r = .295; p< .05). Leukocyte [Zn] exhibited a significant correlation with MT-2A mRNA level (r = .765; p< .001). Higher levels of MT-2A mRNA was found in primiparous mothers (n= 16; M= 1.108, SD= 1.273) compared to multiparous mothers (n= 30; M= 0.581, SD= 0.711). Path analysis showed that leukocyte [Zn] has a greater influence (regression weight .84; p= .000) on MT-2A mRNA expression compared to gravida (regression weight -.18; p= .021). Finally it is concluded that, in addition to circulatory Zn, gravida contributes to the MT-2A mRNA expression. 1, NOOR LIDE 2, 3, MARC DE LEY4, MOHAMMAD TARIQUR 1 2013 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/35414/1/MSBMB38_Doc_for_APAR2.pdf Rahman, Mohammad Tariqur and Youssof, Ayman Lee and Abu Kassim , Noor Lide and Rashid, Siti Aishah and De Ley, Marc (2013) Human cord blood leukocyte metallothionein-2A MRNA expression is associated with primiparaous and multiparous childbirth. In: 38th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology, 28-29 August 2013, Putrajaya Marriott Hotel and Spa, Putrajaya, Malaysia. http://www.msbmb.org |
| spellingShingle | QH301 Biology Rahman, Mohammad Tariqur Youssof, Ayman Lee Abu Kassim , Noor Lide Rashid, Siti Aishah De Ley, Marc Human cord blood leukocyte metallothionein-2A MRNA expression is associated with primiparaous and multiparous childbirth |
| title | Human cord blood leukocyte metallothionein-2A MRNA expression is associated with primiparaous and multiparous childbirth |
| title_full | Human cord blood leukocyte metallothionein-2A MRNA expression is associated with primiparaous and multiparous childbirth |
| title_fullStr | Human cord blood leukocyte metallothionein-2A MRNA expression is associated with primiparaous and multiparous childbirth |
| title_full_unstemmed | Human cord blood leukocyte metallothionein-2A MRNA expression is associated with primiparaous and multiparous childbirth |
| title_short | Human cord blood leukocyte metallothionein-2A MRNA expression is associated with primiparaous and multiparous childbirth |
| title_sort | human cord blood leukocyte metallothionein-2a mrna expression is associated with primiparaous and multiparous childbirth |
| topic | QH301 Biology |
| url | http://irep.iium.edu.my/35414/ http://irep.iium.edu.my/35414/ http://irep.iium.edu.my/35414/1/MSBMB38_Doc_for_APAR2.pdf |