Pilot scale production of lipase using palm oil mill effluent as basal medium
Lipase noted as one of the most important enzymes which has widely industrial uses. Production of low cost enzyme using potential raw material will significantly enhance and attractive to the enzyme market. A local residue of palm oil mill effluent (POME) abundantly found and can be used as a...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/35000/ http://irep.iium.edu.my/35000/1/ICBioE-14-Devi.PDF |
| _version_ | 1848780994037940224 |
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| author | Alam, Md. Zahangir Ratna, Devi Salleh, Md. Noor |
| author_facet | Alam, Md. Zahangir Ratna, Devi Salleh, Md. Noor |
| author_sort | Alam, Md. Zahangir |
| building | IIUM Repository |
| collection | Online Access |
| description | Lipase noted as one of the most important enzymes which has widely industrial uses. Production of low cost enzyme
using potential raw material will significantly enhance and attractive to the enzyme market. A local residue of palm oil
mill effluent (POME) abundantly found and can be used as a basal medium for lipase production. The main objective
of this study is to develop a scale up strategy of lipase production (300L of stirred tank bioreactor) for industrial uses.
The lipase production was carried out by liquid state bioconversion of POME whereas Candida cylindracea as the
microorganism. The fermentation media composition was based on the optimization from the previous study with
1%TSS based POME, 0.5% (W/V) peptone, 0.7% (V/V) Tween-80 and 2.2% inoculums. The fermentation was carried
out for 48 hours at 30°C and pH 6. The results showed that maximum lipase production 5.72U/ml and 21.34 U/ml was
determined with the scale up strategy of tip speed and P/V constant respectively. |
| first_indexed | 2025-11-14T15:42:30Z |
| format | Proceeding Paper |
| id | iium-35000 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:42:30Z |
| publishDate | 2013 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-350002014-01-28T01:41:38Z http://irep.iium.edu.my/35000/ Pilot scale production of lipase using palm oil mill effluent as basal medium Alam, Md. Zahangir Ratna, Devi Salleh, Md. Noor TP248.13 Biotechnology Lipase noted as one of the most important enzymes which has widely industrial uses. Production of low cost enzyme using potential raw material will significantly enhance and attractive to the enzyme market. A local residue of palm oil mill effluent (POME) abundantly found and can be used as a basal medium for lipase production. The main objective of this study is to develop a scale up strategy of lipase production (300L of stirred tank bioreactor) for industrial uses. The lipase production was carried out by liquid state bioconversion of POME whereas Candida cylindracea as the microorganism. The fermentation media composition was based on the optimization from the previous study with 1%TSS based POME, 0.5% (W/V) peptone, 0.7% (V/V) Tween-80 and 2.2% inoculums. The fermentation was carried out for 48 hours at 30°C and pH 6. The results showed that maximum lipase production 5.72U/ml and 21.34 U/ml was determined with the scale up strategy of tip speed and P/V constant respectively. 2013 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/35000/1/ICBioE-14-Devi.PDF Alam, Md. Zahangir and Ratna, Devi and Salleh, Md. Noor (2013) Pilot scale production of lipase using palm oil mill effluent as basal medium. In: International Conference on Biotechnology Engineering (ICBioE 2013), 2 – 4 July 2013, Kuala Lumpur, Malaysia. |
| spellingShingle | TP248.13 Biotechnology Alam, Md. Zahangir Ratna, Devi Salleh, Md. Noor Pilot scale production of lipase using palm oil mill effluent as basal medium |
| title | Pilot scale production of lipase using palm oil mill effluent as basal medium |
| title_full | Pilot scale production of lipase using palm oil mill effluent as basal medium |
| title_fullStr | Pilot scale production of lipase using palm oil mill effluent as basal medium |
| title_full_unstemmed | Pilot scale production of lipase using palm oil mill effluent as basal medium |
| title_short | Pilot scale production of lipase using palm oil mill effluent as basal medium |
| title_sort | pilot scale production of lipase using palm oil mill effluent as basal medium |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/35000/ http://irep.iium.edu.my/35000/1/ICBioE-14-Devi.PDF |