Statistical screening and optimization of factors influence lipase production from palm kernel cake using Candida Cylindracea by solid-state bioconversion
Several factors affecting lipase production by Candida cylindracea were studied using palm kernel cake (PKC) as the basal medium. The factors included for media components: peptone, yeast extract, glucose, NaNO3, MgSO4, olive oil and Tween-80 and process conditions: inoculum, temperature, pH...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/34999/ http://irep.iium.edu.my/34999/1/ICBioE-14-amal.PDF |
| _version_ | 1848780993773699072 |
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| author | Elgharbawy, Amal A.M. Alam, Md. Zahangir Hamzah, Mohd. Salleh |
| author_facet | Elgharbawy, Amal A.M. Alam, Md. Zahangir Hamzah, Mohd. Salleh |
| author_sort | Elgharbawy, Amal A.M. |
| building | IIUM Repository |
| collection | Online Access |
| description | Several factors affecting lipase production by Candida cylindracea were studied using palm kernel
cake (PKC) as the basal medium. The factors included for media components: peptone, yeast
extract, glucose, NaNO3, MgSO4, olive oil and Tween-80 and process conditions: inoculum,
temperature, pH and moisture content. Using Plackett–Burman (PB) design for screening, the
highest lipase activity obtained was 9.41 U/ml in the absence of glucose, peptone and MgSO4. The
factors that contributed the most in lipase production were further investigated using one-factor-at-
a-time (OFAT) experiments in order to find the optimal conditions for the lipase productivity. An
activity of 30.0 U/ml was obtained after 144h of fermentation by the end of this study which was
used for optimization afterword. |
| first_indexed | 2025-11-14T15:42:30Z |
| format | Proceeding Paper |
| id | iium-34999 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:42:30Z |
| publishDate | 2013 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-349992021-06-29T02:59:31Z http://irep.iium.edu.my/34999/ Statistical screening and optimization of factors influence lipase production from palm kernel cake using Candida Cylindracea by solid-state bioconversion Elgharbawy, Amal A.M. Alam, Md. Zahangir Hamzah, Mohd. Salleh TP248.13 Biotechnology Several factors affecting lipase production by Candida cylindracea were studied using palm kernel cake (PKC) as the basal medium. The factors included for media components: peptone, yeast extract, glucose, NaNO3, MgSO4, olive oil and Tween-80 and process conditions: inoculum, temperature, pH and moisture content. Using Plackett–Burman (PB) design for screening, the highest lipase activity obtained was 9.41 U/ml in the absence of glucose, peptone and MgSO4. The factors that contributed the most in lipase production were further investigated using one-factor-at- a-time (OFAT) experiments in order to find the optimal conditions for the lipase productivity. An activity of 30.0 U/ml was obtained after 144h of fermentation by the end of this study which was used for optimization afterword. 2013 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/34999/1/ICBioE-14-amal.PDF Elgharbawy, Amal A.M. and Alam, Md. Zahangir and Hamzah, Mohd. Salleh (2013) Statistical screening and optimization of factors influence lipase production from palm kernel cake using Candida Cylindracea by solid-state bioconversion. In: International Conference on Biotechnology Engineering (ICBioE 2013), 2 – 4 July 2013, Kuala Lumpur, Malaysia. |
| spellingShingle | TP248.13 Biotechnology Elgharbawy, Amal A.M. Alam, Md. Zahangir Hamzah, Mohd. Salleh Statistical screening and optimization of factors influence lipase production from palm kernel cake using Candida Cylindracea by solid-state bioconversion |
| title | Statistical screening and optimization of factors influence lipase production from palm kernel cake using Candida Cylindracea by solid-state bioconversion |
| title_full | Statistical screening and optimization of factors influence lipase production from palm kernel cake using Candida Cylindracea by solid-state bioconversion |
| title_fullStr | Statistical screening and optimization of factors influence lipase production from palm kernel cake using Candida Cylindracea by solid-state bioconversion |
| title_full_unstemmed | Statistical screening and optimization of factors influence lipase production from palm kernel cake using Candida Cylindracea by solid-state bioconversion |
| title_short | Statistical screening and optimization of factors influence lipase production from palm kernel cake using Candida Cylindracea by solid-state bioconversion |
| title_sort | statistical screening and optimization of factors influence lipase production from palm kernel cake using candida cylindracea by solid-state bioconversion |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/34999/ http://irep.iium.edu.my/34999/1/ICBioE-14-amal.PDF |