Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger
A number of media components influencing α-amylase production by Aspergillus niger O103A were studied using local bitter variety of cassava (Manihot escuelenta) as the main substrate. The media components (cassava, maltose, glucose, yeast extract, urea, NH4NO3, KH2PO4, CaCl2, MgSO4.7H2O, Na...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/34998/ http://irep.iium.edu.my/34998/1/ICBioE-14-abu_bakir.PDF |
| _version_ | 1848780993462272000 |
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| author | I.Khalaf, Abu Bakir Alam, Md. Zahangir Hamzah, Mohd. Salleh |
| author_facet | I.Khalaf, Abu Bakir Alam, Md. Zahangir Hamzah, Mohd. Salleh |
| author_sort | I.Khalaf, Abu Bakir |
| building | IIUM Repository |
| collection | Online Access |
| description | A number of media components influencing α-amylase production by Aspergillus niger
O103A were studied using local bitter variety of cassava (Manihot escuelenta) as the main
substrate. The media components (cassava, maltose, glucose, yeast extract, urea, NH4NO3,
KH2PO4, CaCl2, MgSO4.7H2O, NaCl, and FeSO4.7H2O) were analyzed using Plackett–
Burman design. Optimal concentrations of the components contributing positively to
enzyme production were further determined using one factor at a time (OFAT) method.
Among different nitrogen and supplementary carbon sources tested, yeast extract 2 (g/l) and
maltose 3 (g/L) were most effective for enzyme production. Where the optimized media
resulted in higher enzyme titre of 42 U/ml. The results indicated the efficiency of using PB
design and (OFAT) methodology for screening and optimization processes. |
| first_indexed | 2025-11-14T15:42:30Z |
| format | Proceeding Paper |
| id | iium-34998 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:42:30Z |
| publishDate | 2013 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-349982021-06-29T03:03:02Z http://irep.iium.edu.my/34998/ Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger I.Khalaf, Abu Bakir Alam, Md. Zahangir Hamzah, Mohd. Salleh TP248.13 Biotechnology A number of media components influencing α-amylase production by Aspergillus niger O103A were studied using local bitter variety of cassava (Manihot escuelenta) as the main substrate. The media components (cassava, maltose, glucose, yeast extract, urea, NH4NO3, KH2PO4, CaCl2, MgSO4.7H2O, NaCl, and FeSO4.7H2O) were analyzed using Plackett– Burman design. Optimal concentrations of the components contributing positively to enzyme production were further determined using one factor at a time (OFAT) method. Among different nitrogen and supplementary carbon sources tested, yeast extract 2 (g/l) and maltose 3 (g/L) were most effective for enzyme production. Where the optimized media resulted in higher enzyme titre of 42 U/ml. The results indicated the efficiency of using PB design and (OFAT) methodology for screening and optimization processes. 2013 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/34998/1/ICBioE-14-abu_bakir.PDF I.Khalaf, Abu Bakir and Alam, Md. Zahangir and Hamzah, Mohd. Salleh (2013) Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger. In: 3rd International Conference on Biotechnology Engineering (ICBIoE 2013), 2-4 July 2013, Kuala Lumpur, Malaysia. |
| spellingShingle | TP248.13 Biotechnology I.Khalaf, Abu Bakir Alam, Md. Zahangir Hamzah, Mohd. Salleh Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger |
| title | Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger |
| title_full | Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger |
| title_fullStr | Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger |
| title_full_unstemmed | Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger |
| title_short | Optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger |
| title_sort | optimization of media composition for α-amylase production in liquid state fermentation of bitter cassava by aspergillus niger |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/34998/ http://irep.iium.edu.my/34998/1/ICBioE-14-abu_bakir.PDF |