In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load
Hepatitis C is a viral disease caused by hepatitis C virus. Patients infected with the virus may develop chronic hepatitis, who were remain asymptomatic. Our objective is to established a sensitive and reliable assay for the detection of HCV-RNA in serum. Sera were collected from 39 chronic HCV pati...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/34879/ http://irep.iium.edu.my/34879/1/IRIIE-2013-Abstact-Category.pdf |
| _version_ | 1848780974646624256 |
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| author | Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdullah, Nasuruddin |
| author_facet | Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdullah, Nasuruddin |
| author_sort | Hamzah, Hairul Aini |
| building | IIUM Repository |
| collection | Online Access |
| description | Hepatitis C is a viral disease caused by hepatitis C virus. Patients infected with the virus may develop chronic hepatitis, who were remain asymptomatic. Our objective is to established a sensitive and reliable assay for the detection of HCV-RNA in serum. Sera were collected from 39 chronic HCV patients and 53 from patients with negative anti-HCV. Viral RNA was extrcated and subjected for RT-PCR or semi-nested RT-PCR. Results showed high sensitivity of the assay which can detect up to 2.15 IU/ml of viral load. The specificity of the assay was also evaluated. Thus, the extablished protocol was reliable for the detection of HCV-RNA from serum. |
| first_indexed | 2025-11-14T15:42:12Z |
| format | Proceeding Paper |
| id | iium-34879 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:42:12Z |
| publishDate | 2013 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-348792014-01-28T04:48:55Z http://irep.iium.edu.my/34879/ In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdullah, Nasuruddin QR355 Virology Hepatitis C is a viral disease caused by hepatitis C virus. Patients infected with the virus may develop chronic hepatitis, who were remain asymptomatic. Our objective is to established a sensitive and reliable assay for the detection of HCV-RNA in serum. Sera were collected from 39 chronic HCV patients and 53 from patients with negative anti-HCV. Viral RNA was extrcated and subjected for RT-PCR or semi-nested RT-PCR. Results showed high sensitivity of the assay which can detect up to 2.15 IU/ml of viral load. The specificity of the assay was also evaluated. Thus, the extablished protocol was reliable for the detection of HCV-RNA from serum. 2013 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/34879/1/IRIIE-2013-Abstact-Category.pdf Hamzah, Hairul Aini and Mustafa Mahmoud, Mohammed Imad Al-Deen and Abdullah, Nasuruddin (2013) In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load. In: IIUM Research, Invention and Innovation Exhibition 2013, 19 - 20 February 2013, Cultural Activity Centre (CAC) and KAED Gallery, IIUM. |
| spellingShingle | QR355 Virology Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Abdullah, Nasuruddin In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load |
| title | In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load |
| title_full | In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load |
| title_fullStr | In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load |
| title_full_unstemmed | In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load |
| title_short | In-house semi-nested reverse transcription polymerase chain reaction for the detection of HCV-RNA in serum with low viral load |
| title_sort | in-house semi-nested reverse transcription polymerase chain reaction for the detection of hcv-rna in serum with low viral load |
| topic | QR355 Virology |
| url | http://irep.iium.edu.my/34879/ http://irep.iium.edu.my/34879/1/IRIIE-2013-Abstact-Category.pdf |