A study of Paraoxonase-1 192 gene polymorphism in coronary artery disease

Paraoxonase-1 (PON-1) is an HDL-associated enzyme which has been implicated in the pathogenesis of atherosclerosis by preventing lipid peroxide generation. The population variability in this enzyme activity is attributed to polymorphism in PON-1 gene. Recent studies have suggested that PON-1 Q192R...

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Main Authors: Muhammad Musa, Nurul Ashikin, Abdullah, Nor Zamzila, A.Talib, Norlelawati, Nik Ahmad, Nik Nur Fatnoon
Format: Proceeding Paper
Language:English
Published: 2013
Subjects:
Online Access:http://irep.iium.edu.my/34291/
http://irep.iium.edu.my/34291/1/poster_abstract_for_MSAB_symposium_2013.jpg
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author Muhammad Musa, Nurul Ashikin
Abdullah, Nor Zamzila
A.Talib, Norlelawati
Nik Ahmad, Nik Nur Fatnoon
author_facet Muhammad Musa, Nurul Ashikin
Abdullah, Nor Zamzila
A.Talib, Norlelawati
Nik Ahmad, Nik Nur Fatnoon
author_sort Muhammad Musa, Nurul Ashikin
building IIUM Repository
collection Online Access
description Paraoxonase-1 (PON-1) is an HDL-associated enzyme which has been implicated in the pathogenesis of atherosclerosis by preventing lipid peroxide generation. The population variability in this enzyme activity is attributed to polymorphism in PON-1 gene. Recent studies have suggested that PON-1 Q192R polymorphism is associated with coronary artery disease (CAD) in different ethnic populations. However to this date, there is no such study in Malaysia. The present study looks at PON-1 Q192R gene polymorphism among CAD patients and healthy controls in Kuantan, Pahang. This study consisted of 187 CAD patients and 188 healthy subjects. PON-1 Q192R genotypes were determined by polymerase chain reaction (PCR) and AlwI restriction enzyme digestion. The Q192R polymorphism was significantly associated with CAD (p = 0.004), where the 192R allele was found to be more frequent in CAD patients than controls (0.618 vs 0.513) with QR genotype being the commonest (45.5%), followed by RR (39.0%) and QQ (15.5%) genotypes. In conclusion, the PON-1 Q192R polymorphism is significantly associated with CAD. This finding indicates that this gene variation is a useful genetic marker for CAD in our population. However, a multicentre study may be required to confirm our findings in Malaysian population.
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institution International Islamic University Malaysia
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language English
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spelling iium-342912014-01-16T06:14:34Z http://irep.iium.edu.my/34291/ A study of Paraoxonase-1 192 gene polymorphism in coronary artery disease Muhammad Musa, Nurul Ashikin Abdullah, Nor Zamzila A.Talib, Norlelawati Nik Ahmad, Nik Nur Fatnoon RB Pathology Paraoxonase-1 (PON-1) is an HDL-associated enzyme which has been implicated in the pathogenesis of atherosclerosis by preventing lipid peroxide generation. The population variability in this enzyme activity is attributed to polymorphism in PON-1 gene. Recent studies have suggested that PON-1 Q192R polymorphism is associated with coronary artery disease (CAD) in different ethnic populations. However to this date, there is no such study in Malaysia. The present study looks at PON-1 Q192R gene polymorphism among CAD patients and healthy controls in Kuantan, Pahang. This study consisted of 187 CAD patients and 188 healthy subjects. PON-1 Q192R genotypes were determined by polymerase chain reaction (PCR) and AlwI restriction enzyme digestion. The Q192R polymorphism was significantly associated with CAD (p = 0.004), where the 192R allele was found to be more frequent in CAD patients than controls (0.618 vs 0.513) with QR genotype being the commonest (45.5%), followed by RR (39.0%) and QQ (15.5%) genotypes. In conclusion, the PON-1 Q192R polymorphism is significantly associated with CAD. This finding indicates that this gene variation is a useful genetic marker for CAD in our population. However, a multicentre study may be required to confirm our findings in Malaysian population. 2013 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/34291/1/poster_abstract_for_MSAB_symposium_2013.jpg Muhammad Musa, Nurul Ashikin and Abdullah, Nor Zamzila and A.Talib, Norlelawati and Nik Ahmad, Nik Nur Fatnoon (2013) A study of Paraoxonase-1 192 gene polymorphism in coronary artery disease. In: 9th National Biology Symposium, 28-30 May 2013, Thistle Port Dickson Resort, Negeri Sembilan.. (Unpublished)
spellingShingle RB Pathology
Muhammad Musa, Nurul Ashikin
Abdullah, Nor Zamzila
A.Talib, Norlelawati
Nik Ahmad, Nik Nur Fatnoon
A study of Paraoxonase-1 192 gene polymorphism in coronary artery disease
title A study of Paraoxonase-1 192 gene polymorphism in coronary artery disease
title_full A study of Paraoxonase-1 192 gene polymorphism in coronary artery disease
title_fullStr A study of Paraoxonase-1 192 gene polymorphism in coronary artery disease
title_full_unstemmed A study of Paraoxonase-1 192 gene polymorphism in coronary artery disease
title_short A study of Paraoxonase-1 192 gene polymorphism in coronary artery disease
title_sort study of paraoxonase-1 192 gene polymorphism in coronary artery disease
topic RB Pathology
url http://irep.iium.edu.my/34291/
http://irep.iium.edu.my/34291/1/poster_abstract_for_MSAB_symposium_2013.jpg