Synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of ABCG2 expression in plasma cells
ABCG2 is a transporter preferentially expressed in a primitive subpopulation of cells and recently reported as a surviving factor for trophoblasts. To date, manner of ABCG2 expression in lymphoid tissues is not known. Immunohistochemically, strong ABCG2 expression was found in a small proportion of...
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2009
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| Online Access: | http://irep.iium.edu.my/34105/ http://irep.iium.edu.my/34105/1/2009.pdf |
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| author | Nakamichi, Naoto Morii, Eiichi Ikeda, Jun-Ichiro Qiu, Ying Mamat, Suhana Tian, Tian Fukuhara, Shirou Aozasa, Katsuyuki |
| author_facet | Nakamichi, Naoto Morii, Eiichi Ikeda, Jun-Ichiro Qiu, Ying Mamat, Suhana Tian, Tian Fukuhara, Shirou Aozasa, Katsuyuki |
| author_sort | Nakamichi, Naoto |
| building | IIUM Repository |
| collection | Online Access |
| description | ABCG2 is a transporter preferentially expressed in a primitive subpopulation of cells and recently reported as a surviving factor for trophoblasts. To date, manner of ABCG2 expression in lymphoid tissues is not known. Immunohistochemically, strong ABCG2 expression was found in a small proportion of plasma cells mainly located in the interfollicular space of lymphoid tissues. The number of ABCG2-high plasma cells increased in interleukin-6- (IL-6) rich lesions, such as Castleman's disease of plasma cell type. Plasma cells are subjected to endoplasmic reticulum (ER) stress when excess proteins are synthesized, and IL-6 stimulates protein synthesis. Therefore, the effect of IL-6 and ER stress on ABCG2 expression in plasma cells was examined. The expression level of ABCG2 increased by treatment with either IL-6 or ER stress inducers, and further increased with both. The promoter analysis revealed that the effect of IL-6 and ER stress inducers was mediated through the site overlapping XBP-1 and HIF-1 binding sequences. Knocked-down of ABCG2 by siRNA or ABCG2 inhibitor reduced plasma cell viability under ER stress. These suggest that ABCG2 is a surviving factor for plasma cells. To our knowledge, this is the first study reporting the effect of ER stress on ABCG2 expression. |
| first_indexed | 2025-11-14T15:40:19Z |
| format | Article |
| id | iium-34105 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:40:19Z |
| publishDate | 2009 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
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| spelling | iium-341052014-01-13T06:24:45Z http://irep.iium.edu.my/34105/ Synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of ABCG2 expression in plasma cells Nakamichi, Naoto Morii, Eiichi Ikeda, Jun-Ichiro Qiu, Ying Mamat, Suhana Tian, Tian Fukuhara, Shirou Aozasa, Katsuyuki RB Pathology ABCG2 is a transporter preferentially expressed in a primitive subpopulation of cells and recently reported as a surviving factor for trophoblasts. To date, manner of ABCG2 expression in lymphoid tissues is not known. Immunohistochemically, strong ABCG2 expression was found in a small proportion of plasma cells mainly located in the interfollicular space of lymphoid tissues. The number of ABCG2-high plasma cells increased in interleukin-6- (IL-6) rich lesions, such as Castleman's disease of plasma cell type. Plasma cells are subjected to endoplasmic reticulum (ER) stress when excess proteins are synthesized, and IL-6 stimulates protein synthesis. Therefore, the effect of IL-6 and ER stress on ABCG2 expression in plasma cells was examined. The expression level of ABCG2 increased by treatment with either IL-6 or ER stress inducers, and further increased with both. The promoter analysis revealed that the effect of IL-6 and ER stress inducers was mediated through the site overlapping XBP-1 and HIF-1 binding sequences. Knocked-down of ABCG2 by siRNA or ABCG2 inhibitor reduced plasma cell viability under ER stress. These suggest that ABCG2 is a surviving factor for plasma cells. To our knowledge, this is the first study reporting the effect of ER stress on ABCG2 expression. Nature Publishing Group 2009-01-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/34105/1/2009.pdf Nakamichi, Naoto and Morii, Eiichi and Ikeda, Jun-Ichiro and Qiu, Ying and Mamat, Suhana and Tian, Tian and Fukuhara, Shirou and Aozasa, Katsuyuki (2009) Synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of ABCG2 expression in plasma cells. Laboratory Investigation, 89 (3). pp. 327-336. ISSN 1530-0307 (O), 0023-6837 (P) http://www.nature.com/labinvest/journal/v89/n3/full/labinvest2008157a.html 10.1038/labinvest.2008.157 |
| spellingShingle | RB Pathology Nakamichi, Naoto Morii, Eiichi Ikeda, Jun-Ichiro Qiu, Ying Mamat, Suhana Tian, Tian Fukuhara, Shirou Aozasa, Katsuyuki Synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of ABCG2 expression in plasma cells |
| title | Synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of ABCG2 expression in plasma cells |
| title_full | Synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of ABCG2 expression in plasma cells |
| title_fullStr | Synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of ABCG2 expression in plasma cells |
| title_full_unstemmed | Synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of ABCG2 expression in plasma cells |
| title_short | Synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of ABCG2 expression in plasma cells |
| title_sort | synergistic effect of interleukin-6 and endoplasmic reticulum stress inducers on the high level of abcg2 expression in plasma cells |
| topic | RB Pathology |
| url | http://irep.iium.edu.my/34105/ http://irep.iium.edu.my/34105/ http://irep.iium.edu.my/34105/ http://irep.iium.edu.my/34105/1/2009.pdf |