Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells
Tumor cells with tumorigenic potential might be limited to a small population of cells, called cancer-initiating cells (CICs). CICs efficiently form colonies in vitro, yield both CIC and non-CIC populations, maintain reactive oxygen species (ROS) at low levels, show high aldehyde dehydrogenase (ALDH...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2012
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/34103/ http://irep.iium.edu.my/34103/1/2012.pdf |
| _version_ | 1848780856366202880 |
|---|---|
| author | Ikeda, Jun-ichiro Mamat, Suhana Tian, Tian Wang, Yi Luo, Wenjuan Rahadiani, Nur Aozasa, Katsuyuki Morii, Eiichi |
| author_facet | Ikeda, Jun-ichiro Mamat, Suhana Tian, Tian Wang, Yi Luo, Wenjuan Rahadiani, Nur Aozasa, Katsuyuki Morii, Eiichi |
| author_sort | Ikeda, Jun-ichiro |
| building | IIUM Repository |
| collection | Online Access |
| description | Tumor cells with tumorigenic potential might be limited to a small population of cells, called cancer-initiating cells (CICs). CICs efficiently form colonies in vitro, yield both CIC and non-CIC populations, maintain reactive oxygen species (ROS) at low levels, show high aldehyde dehydrogenase (ALDH) activity, and are mostly in a quiescent state of the cell cycle. CICs of Hodgkin lymphoma (HL) are small in size, with low levels of ROS. The relationship between ROS level and ALDH activity in CICs was examined in HL cell lines. ROS-low and ALDH-high populations formed colonies in semi-solid cultures more efficiently than ROS-high and ALDH-low populations. ALDH-high populations yielded both ALDH-low and -high populations, whereas ALDH-low populations rarely yielded an ALDH-high population. The number of cells in a quiescent state was significantly greater in ROS-low than in ROS-high cells, whereas that of ALDH-high and ALDH-low cells was comparable to each other. These findings show that ALDH-high and ROS-low cells share CIC-like potential, but they differ in their cell cycle status, suggesting that CICs are comprised of cells with heterogeneous characteristics. |
| first_indexed | 2025-11-14T15:40:19Z |
| format | Article |
| id | iium-34103 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:40:19Z |
| publishDate | 2012 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-341032014-01-10T08:30:53Z http://irep.iium.edu.my/34103/ Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells Ikeda, Jun-ichiro Mamat, Suhana Tian, Tian Wang, Yi Luo, Wenjuan Rahadiani, Nur Aozasa, Katsuyuki Morii, Eiichi RB Pathology Tumor cells with tumorigenic potential might be limited to a small population of cells, called cancer-initiating cells (CICs). CICs efficiently form colonies in vitro, yield both CIC and non-CIC populations, maintain reactive oxygen species (ROS) at low levels, show high aldehyde dehydrogenase (ALDH) activity, and are mostly in a quiescent state of the cell cycle. CICs of Hodgkin lymphoma (HL) are small in size, with low levels of ROS. The relationship between ROS level and ALDH activity in CICs was examined in HL cell lines. ROS-low and ALDH-high populations formed colonies in semi-solid cultures more efficiently than ROS-high and ALDH-low populations. ALDH-high populations yielded both ALDH-low and -high populations, whereas ALDH-low populations rarely yielded an ALDH-high population. The number of cells in a quiescent state was significantly greater in ROS-low than in ROS-high cells, whereas that of ALDH-high and ALDH-low cells was comparable to each other. These findings show that ALDH-high and ROS-low cells share CIC-like potential, but they differ in their cell cycle status, suggesting that CICs are comprised of cells with heterogeneous characteristics. Nature Publishing Group 2012 Article PeerReviewed application/pdf en http://irep.iium.edu.my/34103/1/2012.pdf Ikeda, Jun-ichiro and Mamat, Suhana and Tian, Tian and Wang, Yi and Luo, Wenjuan and Rahadiani, Nur and Aozasa, Katsuyuki and Morii, Eiichi (2012) Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells. Laboratory Investigation, 92 (4). pp. 606-6014. ISSN 1530-0307 (E-ISSN), 0023-6837 (ISSN) doi:10.1038/labinvest.2012.4 |
| spellingShingle | RB Pathology Ikeda, Jun-ichiro Mamat, Suhana Tian, Tian Wang, Yi Luo, Wenjuan Rahadiani, Nur Aozasa, Katsuyuki Morii, Eiichi Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells |
| title | Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells |
| title_full | Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells |
| title_fullStr | Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells |
| title_full_unstemmed | Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells |
| title_short | Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells |
| title_sort | reactive oxygen species and aldehyde dehydrogenase activity in hodgkin lymphoma cells |
| topic | RB Pathology |
| url | http://irep.iium.edu.my/34103/ http://irep.iium.edu.my/34103/ http://irep.iium.edu.my/34103/1/2012.pdf |