Celecoxib as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats

Objectives: Reduced cerebral blood flow (CBF)has been associated with neurodegenerative disorders. Since neuroinflammation is thought to play a significant role in chronic degenerative neurological disorders like Alzheimer's disease, the present study was planned to,assess the neuroprotective...

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Main Authors: Saxena, Anil Kumar, Mohd Noor, Mohd Fadly, A.Talib, Norlelawati
Format: Proceeding Paper
Language:English
English
English
Published: 2013
Subjects:
Online Access:http://irep.iium.edu.my/33808/
http://irep.iium.edu.my/33808/1/Oral_Presentation_Certificate.pdf
http://irep.iium.edu.my/33808/2/Abstract.pdf
http://irep.iium.edu.my/33808/3/Cover_Page.pdf
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author Saxena, Anil Kumar
Mohd Noor, Mohd Fadly
A.Talib, Norlelawati
author_facet Saxena, Anil Kumar
Mohd Noor, Mohd Fadly
A.Talib, Norlelawati
author_sort Saxena, Anil Kumar
building IIUM Repository
collection Online Access
description Objectives: Reduced cerebral blood flow (CBF)has been associated with neurodegenerative disorders. Since neuroinflammation is thought to play a significant role in chronic degenerative neurological disorders like Alzheimer's disease, the present study was planned to,assess the neuroprotective role of celecoxib in Alzheimer's model of rats(2VO). Methods:Experimentally,a condition of chronic cerebral hypoperfusion due to reduced CBF can be induced by permanent bilateral occlusion of common carotid arteries (2VO)in rats. After 1 week of acclimatization,fifteen Sprague Dawley rats weighing 200-250 g were equally divided into three groups. Group A served as - sham control, Group B - 2VO, and Group C - 2VO-C treated daily with celecoxib 50 mg/kg, orally following 2VO. On 8th week, all the rats were euthanized and the hippocampi were isolated. Viable neuronal cells in the hippocampal CA-1 region were counted and hippocampal COX-2 mRNA expression and prostaglandin E2(PGE-2)levels were estimated. Results: There was a significant difference in neuronal cell death, increase in COX-2 mRNA expression and PGE-2 levels in 2VO group as compared to sham control group. In celecoxib-treated 2VO-C rats,the viable neuronal cell count of the hippocampal CA-l region was significantly higher as compared to the untreated 2VO group. The hippocampal COX-2 mRNA expression and hippocampal PGE-2 levels were found to be significantly lower in the celecoxib-treated 2VO rats as compared to untreated 2VO rats. Conclusions: The results indicate that celecoxib could be successfully used in the management of Alzheimer's disease. No conflict of interest.
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institution International Islamic University Malaysia
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language English
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spelling iium-338082020-11-09T07:28:54Z http://irep.iium.edu.my/33808/ Celecoxib as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats Saxena, Anil Kumar Mohd Noor, Mohd Fadly A.Talib, Norlelawati R Medicine (General) RC321 Neuroscience. Biological psychiatry. Neuropsychiatry RM300 Drugs and their action Objectives: Reduced cerebral blood flow (CBF)has been associated with neurodegenerative disorders. Since neuroinflammation is thought to play a significant role in chronic degenerative neurological disorders like Alzheimer's disease, the present study was planned to,assess the neuroprotective role of celecoxib in Alzheimer's model of rats(2VO). Methods:Experimentally,a condition of chronic cerebral hypoperfusion due to reduced CBF can be induced by permanent bilateral occlusion of common carotid arteries (2VO)in rats. After 1 week of acclimatization,fifteen Sprague Dawley rats weighing 200-250 g were equally divided into three groups. Group A served as - sham control, Group B - 2VO, and Group C - 2VO-C treated daily with celecoxib 50 mg/kg, orally following 2VO. On 8th week, all the rats were euthanized and the hippocampi were isolated. Viable neuronal cells in the hippocampal CA-1 region were counted and hippocampal COX-2 mRNA expression and prostaglandin E2(PGE-2)levels were estimated. Results: There was a significant difference in neuronal cell death, increase in COX-2 mRNA expression and PGE-2 levels in 2VO group as compared to sham control group. In celecoxib-treated 2VO-C rats,the viable neuronal cell count of the hippocampal CA-l region was significantly higher as compared to the untreated 2VO group. The hippocampal COX-2 mRNA expression and hippocampal PGE-2 levels were found to be significantly lower in the celecoxib-treated 2VO rats as compared to untreated 2VO rats. Conclusions: The results indicate that celecoxib could be successfully used in the management of Alzheimer's disease. No conflict of interest. 2013-12-08 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/33808/1/Oral_Presentation_Certificate.pdf application/pdf en http://irep.iium.edu.my/33808/2/Abstract.pdf application/pdf en http://irep.iium.edu.my/33808/3/Cover_Page.pdf Saxena, Anil Kumar and Mohd Noor, Mohd Fadly and A.Talib, Norlelawati (2013) Celecoxib as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats. In: XX World Congress on Parkinson"s Disease and Related Disorders, 8-11 Dec 2013 , Geneva, Switzerland. (Unpublished) http://www.kenes.com/parkinson
spellingShingle R Medicine (General)
RC321 Neuroscience. Biological psychiatry. Neuropsychiatry
RM300 Drugs and their action
Saxena, Anil Kumar
Mohd Noor, Mohd Fadly
A.Talib, Norlelawati
Celecoxib as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats
title Celecoxib as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats
title_full Celecoxib as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats
title_fullStr Celecoxib as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats
title_full_unstemmed Celecoxib as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats
title_short Celecoxib as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats
title_sort celecoxib as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats
topic R Medicine (General)
RC321 Neuroscience. Biological psychiatry. Neuropsychiatry
RM300 Drugs and their action
url http://irep.iium.edu.my/33808/
http://irep.iium.edu.my/33808/
http://irep.iium.edu.my/33808/1/Oral_Presentation_Certificate.pdf
http://irep.iium.edu.my/33808/2/Abstract.pdf
http://irep.iium.edu.my/33808/3/Cover_Page.pdf