Callus cultures: an alternative research tool to study regulatory mechanisms od carotenoid biosynthesis
Every single step happened in the carotenoid biosynthetic pathway in the leaves is regulated by respective genes. It was presumed that these genes would be inherited by the undifferentiated cells (callus) since they were initiated from the intact plants. Thus, the callus is most likely to develop an...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/33637/ http://irep.iium.edu.my/33637/1/WBC_2013.pdf |
| _version_ | 1848780783766994944 |
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| author | Mohd Zaifuddin, Fatimah Azzahra Othman, Rashidi Mohd Hassan, Norazian |
| author_facet | Mohd Zaifuddin, Fatimah Azzahra Othman, Rashidi Mohd Hassan, Norazian |
| author_sort | Mohd Zaifuddin, Fatimah Azzahra |
| building | IIUM Repository |
| collection | Online Access |
| description | Every single step happened in the carotenoid biosynthetic pathway in the leaves is regulated by respective genes. It was presumed that these genes would be inherited by the undifferentiated cells (callus) since they were initiated from the intact plants. Thus, the callus is most likely to develop and respond to internal and external stimuli exactly as the intact plants. Therefore, this study was aimed at establishing callus cultures as an alternative research tool to study the regulatory mechanisms of the carotenoid biosynthesis in plants through a controlled environment. Via HPLC analysis, it was found that different strain of callus cultures accumulated different types of carotenoids as compared to intact plants. The finding from this study suggested that researchers would be able to assess what are the key factors which involved in regulatory mechanisms of individual carotenoid biosynthesis in a particular biology system. Thereafter, future exploration and manipulation of the genetic make-up is made possible to enhance or enrich certain individual carotenoid in food crops. |
| first_indexed | 2025-11-14T15:39:10Z |
| format | Proceeding Paper |
| id | iium-33637 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:39:10Z |
| publishDate | 2013 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-336372021-07-24T15:13:39Z http://irep.iium.edu.my/33637/ Callus cultures: an alternative research tool to study regulatory mechanisms od carotenoid biosynthesis Mohd Zaifuddin, Fatimah Azzahra Othman, Rashidi Mohd Hassan, Norazian RS Pharmacy and materia medica SB Plant culture Every single step happened in the carotenoid biosynthetic pathway in the leaves is regulated by respective genes. It was presumed that these genes would be inherited by the undifferentiated cells (callus) since they were initiated from the intact plants. Thus, the callus is most likely to develop and respond to internal and external stimuli exactly as the intact plants. Therefore, this study was aimed at establishing callus cultures as an alternative research tool to study the regulatory mechanisms of the carotenoid biosynthesis in plants through a controlled environment. Via HPLC analysis, it was found that different strain of callus cultures accumulated different types of carotenoids as compared to intact plants. The finding from this study suggested that researchers would be able to assess what are the key factors which involved in regulatory mechanisms of individual carotenoid biosynthesis in a particular biology system. Thereafter, future exploration and manipulation of the genetic make-up is made possible to enhance or enrich certain individual carotenoid in food crops. 2013 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/33637/1/WBC_2013.pdf Mohd Zaifuddin, Fatimah Azzahra and Othman, Rashidi and Mohd Hassan, Norazian (2013) Callus cultures: an alternative research tool to study regulatory mechanisms od carotenoid biosynthesis. In: Global Biotechnology Congress 2013, 3-6 June 2013, Boston, USA. http://www.worldbiotechcongress.com/abstracts/PP/PP-14-05-2013_128_FatimahAzzahraMohdZaifuddin.htm |
| spellingShingle | RS Pharmacy and materia medica SB Plant culture Mohd Zaifuddin, Fatimah Azzahra Othman, Rashidi Mohd Hassan, Norazian Callus cultures: an alternative research tool to study regulatory mechanisms od carotenoid biosynthesis |
| title | Callus cultures: an alternative research tool to study regulatory mechanisms od carotenoid biosynthesis |
| title_full | Callus cultures: an alternative research tool to study regulatory mechanisms od carotenoid biosynthesis |
| title_fullStr | Callus cultures: an alternative research tool to study regulatory mechanisms od carotenoid biosynthesis |
| title_full_unstemmed | Callus cultures: an alternative research tool to study regulatory mechanisms od carotenoid biosynthesis |
| title_short | Callus cultures: an alternative research tool to study regulatory mechanisms od carotenoid biosynthesis |
| title_sort | callus cultures: an alternative research tool to study regulatory mechanisms od carotenoid biosynthesis |
| topic | RS Pharmacy and materia medica SB Plant culture |
| url | http://irep.iium.edu.my/33637/ http://irep.iium.edu.my/33637/ http://irep.iium.edu.my/33637/1/WBC_2013.pdf |