Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design
Xanthine oxidase (XO) is a key enzyme in hyperuricemia, catalyzing the oxidation of hypoxanthine to xanthine and then to uric acid. Excess serum accumulated with uric acid leads to a type of arthritis known as gout. In this study, development of process conditions for XO inhibitory activity from t...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/33526/ http://irep.iium.edu.my/33526/1/ICBIOE_2.pdf |
| _version_ | 1848780763954151424 |
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| author | Jamal, Parveen Azmi, Saiful Mohammad Nizam Amid, Azura |
| author_facet | Jamal, Parveen Azmi, Saiful Mohammad Nizam Amid, Azura |
| author_sort | Jamal, Parveen |
| building | IIUM Repository |
| collection | Online Access |
| description | Xanthine oxidase (XO) is a key enzyme in hyperuricemia, catalyzing the oxidation of hypoxanthine to
xanthine and then to uric acid. Excess serum accumulated with uric acid leads to a type of arthritis known as
gout. In this study, development of process conditions for XO inhibitory activity from the leaves of Morinda
elliptica was performed by using 70% methanol. Optimization of process parameters such as extraction
temperature (ºC), extraction time (h), agitation speed (rpm) and ratio of sample to solvent (1g/ml) at five
levels was carried out using central composite design (CCD) for the improvement of activity to treat gout.
The analysis of variance demonstrated that the model F-value of 18.31 showed the significance of the model
with R2 of 97.71%. The analysis revealed that the percentage of XO inhibitory activity was improved at 32
ºC, 30 h, 125 rpm and 1 g/15 ml of solvent. The optimized conditions were verified and the percentage of
XO inhibitory activity obtained was 88.93%. The results are encouraging to formulate food, nutraceutical or
pharmaceutical products incorporating natural xanthine oxidase inhibitor (XOI), an alternative to
irresponsive synthetic XOI. |
| first_indexed | 2025-11-14T15:38:51Z |
| format | Proceeding Paper |
| id | iium-33526 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:38:51Z |
| publishDate | 2013 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-335262013-12-19T04:41:39Z http://irep.iium.edu.my/33526/ Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design Jamal, Parveen Azmi, Saiful Mohammad Nizam Amid, Azura TA Engineering (General). Civil engineering (General) Xanthine oxidase (XO) is a key enzyme in hyperuricemia, catalyzing the oxidation of hypoxanthine to xanthine and then to uric acid. Excess serum accumulated with uric acid leads to a type of arthritis known as gout. In this study, development of process conditions for XO inhibitory activity from the leaves of Morinda elliptica was performed by using 70% methanol. Optimization of process parameters such as extraction temperature (ºC), extraction time (h), agitation speed (rpm) and ratio of sample to solvent (1g/ml) at five levels was carried out using central composite design (CCD) for the improvement of activity to treat gout. The analysis of variance demonstrated that the model F-value of 18.31 showed the significance of the model with R2 of 97.71%. The analysis revealed that the percentage of XO inhibitory activity was improved at 32 ºC, 30 h, 125 rpm and 1 g/15 ml of solvent. The optimized conditions were verified and the percentage of XO inhibitory activity obtained was 88.93%. The results are encouraging to formulate food, nutraceutical or pharmaceutical products incorporating natural xanthine oxidase inhibitor (XOI), an alternative to irresponsive synthetic XOI. 2013-07-02 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/33526/1/ICBIOE_2.pdf Jamal, Parveen and Azmi, Saiful Mohammad Nizam and Amid, Azura (2013) Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design. In: International Conference on Biotechnology Engineering (ICBioE 2013), 2 – 4 Jul 2013, Kuala Lumpur, Malaysia. |
| spellingShingle | TA Engineering (General). Civil engineering (General) Jamal, Parveen Azmi, Saiful Mohammad Nizam Amid, Azura Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design |
| title | Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design |
| title_full | Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design |
| title_fullStr | Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design |
| title_full_unstemmed | Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design |
| title_short | Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design |
| title_sort | development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design |
| topic | TA Engineering (General). Civil engineering (General) |
| url | http://irep.iium.edu.my/33526/ http://irep.iium.edu.my/33526/1/ICBIOE_2.pdf |