Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on the Chemometry of 1H Nuclear Magnetic Resonance spectroscopy
The metabolites of three species of Apiaceae, also known as Pegaga, were analyzed utilizing 1H NMR spectroscopy and multivariate data analysis. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) resolved the species, Centella asiatica, Hydrocotyle bonariensis, and Hydrocotyle...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
American Chemical Society
2012
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/33503/ http://irep.iium.edu.my/33503/1/Maulidiani_et_al.%2C_2012.pdf |
| _version_ | 1848780760436178944 |
|---|---|
| author | H., Maulidiani Khatib, Alfi Shaari, Khozirah Abas, Faridah Shitan, Mahendran Kneer, Ralf Neto, Victor Lajis, Nurdin Hj |
| author_facet | H., Maulidiani Khatib, Alfi Shaari, Khozirah Abas, Faridah Shitan, Mahendran Kneer, Ralf Neto, Victor Lajis, Nurdin Hj |
| author_sort | H., Maulidiani |
| building | IIUM Repository |
| collection | Online Access |
| description | The metabolites of three species of Apiaceae, also known as Pegaga, were analyzed utilizing 1H NMR spectroscopy and multivariate data analysis. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) resolved the species, Centella asiatica, Hydrocotyle bonariensis, and Hydrocotyle sibthorpioides, into three clusters. The saponins, asiaticoside and madecassoside, along with chlorogenic acids were the metabolites that contributed most to the separation. Furthermore, the effects of growth-lighting condition to metabolite contents were also investigated. The extracts of C. asiatica grown in full-day light exposure exhibited a stronger radical scavenging activity and contained more triterpenes (asiaticoside and madecassoside), flavonoids, and chlorogenic acids as compared to plants grown in 50% shade. This study established the potential of using a combination of 1HNMRspectroscopy and multivariate data analyses in differentiating three closely related species and the effects of growth lighting, based on their metabolite contents and identification of the markers contributing to their differences. |
| first_indexed | 2025-11-14T15:38:47Z |
| format | Article |
| id | iium-33503 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:38:47Z |
| publishDate | 2012 |
| publisher | American Chemical Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-335032013-12-18T03:20:51Z http://irep.iium.edu.my/33503/ Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on the Chemometry of 1H Nuclear Magnetic Resonance spectroscopy H., Maulidiani Khatib, Alfi Shaari, Khozirah Abas, Faridah Shitan, Mahendran Kneer, Ralf Neto, Victor Lajis, Nurdin Hj QD Chemistry The metabolites of three species of Apiaceae, also known as Pegaga, were analyzed utilizing 1H NMR spectroscopy and multivariate data analysis. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) resolved the species, Centella asiatica, Hydrocotyle bonariensis, and Hydrocotyle sibthorpioides, into three clusters. The saponins, asiaticoside and madecassoside, along with chlorogenic acids were the metabolites that contributed most to the separation. Furthermore, the effects of growth-lighting condition to metabolite contents were also investigated. The extracts of C. asiatica grown in full-day light exposure exhibited a stronger radical scavenging activity and contained more triterpenes (asiaticoside and madecassoside), flavonoids, and chlorogenic acids as compared to plants grown in 50% shade. This study established the potential of using a combination of 1HNMRspectroscopy and multivariate data analyses in differentiating three closely related species and the effects of growth lighting, based on their metabolite contents and identification of the markers contributing to their differences. American Chemical Society 2012-03-02 Article PeerReviewed application/pdf en http://irep.iium.edu.my/33503/1/Maulidiani_et_al.%2C_2012.pdf H., Maulidiani and Khatib, Alfi and Shaari, Khozirah and Abas, Faridah and Shitan, Mahendran and Kneer, Ralf and Neto, Victor and Lajis, Nurdin Hj (2012) Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on the Chemometry of 1H Nuclear Magnetic Resonance spectroscopy. Journal of Agricultural and Food Chemistry, 60 (1). pp. 410-417. ISSN 0021-8561 http://pubs.acs.org/doi/abs/10.1021/jf200270y#aff6 10.1021/jf200270y |
| spellingShingle | QD Chemistry H., Maulidiani Khatib, Alfi Shaari, Khozirah Abas, Faridah Shitan, Mahendran Kneer, Ralf Neto, Victor Lajis, Nurdin Hj Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on the Chemometry of 1H Nuclear Magnetic Resonance spectroscopy |
| title | Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on the Chemometry of 1H Nuclear Magnetic Resonance spectroscopy |
| title_full | Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on the Chemometry of 1H Nuclear Magnetic Resonance spectroscopy |
| title_fullStr | Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on the Chemometry of 1H Nuclear Magnetic Resonance spectroscopy |
| title_full_unstemmed | Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on the Chemometry of 1H Nuclear Magnetic Resonance spectroscopy |
| title_short | Discrimination of three Pegaga (Centella) varieties and determination of growth-lighting effects on metabolites content based on the Chemometry of 1H Nuclear Magnetic Resonance spectroscopy |
| title_sort | discrimination of three pegaga (centella) varieties and determination of growth-lighting effects on metabolites content based on the chemometry of 1h nuclear magnetic resonance spectroscopy |
| topic | QD Chemistry |
| url | http://irep.iium.edu.my/33503/ http://irep.iium.edu.my/33503/ http://irep.iium.edu.my/33503/ http://irep.iium.edu.my/33503/1/Maulidiani_et_al.%2C_2012.pdf |