Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system
A new method for the simultaneous quantification of 12 mycotoxins was developed and optimized using reverse phase high performance liquid chromatography (RP-HPLC) with a photodiode array (PDA) and fluorescence detector (FLD), a photochemical reactor for enhanced detection (PHRED) and post-column d...
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| Format: | Article |
| Language: | English |
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Taylor and Francis
2011
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| Online Access: | http://irep.iium.edu.my/33304/ http://irep.iium.edu.my/33304/1/Soleimany%2C_2011.pdf |
| _version_ | 1848780725531181056 |
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| author | Soleimany, F. Jinap, Selamat Rahmani, Anoshep Khatib, Alfi |
| author_facet | Soleimany, F. Jinap, Selamat Rahmani, Anoshep Khatib, Alfi |
| author_sort | Soleimany, F. |
| building | IIUM Repository |
| collection | Online Access |
| description | A new method for the simultaneous quantification of 12 mycotoxins was developed and optimized using reverse
phase high performance liquid chromatography (RP-HPLC) with a photodiode array (PDA) and fluorescence
detector (FLD), a photochemical reactor for enhanced detection (PHRED) and post-column derivatization. The
mycotoxins included aflatoxins (AFB1, AFB2, AFG1, and AFG2), ochratoxin A (OTA), zearalenone (ZEA),
deoxynivalenol (DON), fumonisins (FB1, FB2, and FB3), T-2 and HT-2 toxins. A double sample extraction with
a phosphate-buffered saline solution (PBS) and methanol was used for co-extraction of mycotoxins, and a
multifunctional immunoaffinity column was used for cleanup. Optimum conditions for separation of the
mycotoxins were obtained to separate 12 mycotoxins in FLD and PDA chromatograms with a high resolution.
The method gave recoveries in the range 72–111% when applied to spiked corn samples. The limits of detection
(LOD) were 0.025 ng/g for AFB1 and AFG1, 0.012 ng/g for AFB2 and AFG2, 0.2 ng/g for OTA, 1.5 ng/g for
ZEA, 6.2 ng/g for FB1, FB3 and HT-2 toxin, 9.4 ng/g for FB2 and T-2 toxin, and 18.7 ng/g for DON. In addition,
the limits of quantification (LOQ) ranged from 0.04 ng/g for AFB2 and AFG2 to 62 ng/g for DON. The method
was successfully applied to the determination of these mycotoxins in 45 cereal samples obtained from the
Malaysian market. The results indicated that the method can be applied for the multi-mycotoxin determination
of cereals. |
| first_indexed | 2025-11-14T15:38:14Z |
| format | Article |
| id | iium-33304 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:38:14Z |
| publishDate | 2011 |
| publisher | Taylor and Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-333042013-12-13T03:24:57Z http://irep.iium.edu.my/33304/ Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system Soleimany, F. Jinap, Selamat Rahmani, Anoshep Khatib, Alfi QD Chemistry A new method for the simultaneous quantification of 12 mycotoxins was developed and optimized using reverse phase high performance liquid chromatography (RP-HPLC) with a photodiode array (PDA) and fluorescence detector (FLD), a photochemical reactor for enhanced detection (PHRED) and post-column derivatization. The mycotoxins included aflatoxins (AFB1, AFB2, AFG1, and AFG2), ochratoxin A (OTA), zearalenone (ZEA), deoxynivalenol (DON), fumonisins (FB1, FB2, and FB3), T-2 and HT-2 toxins. A double sample extraction with a phosphate-buffered saline solution (PBS) and methanol was used for co-extraction of mycotoxins, and a multifunctional immunoaffinity column was used for cleanup. Optimum conditions for separation of the mycotoxins were obtained to separate 12 mycotoxins in FLD and PDA chromatograms with a high resolution. The method gave recoveries in the range 72–111% when applied to spiked corn samples. The limits of detection (LOD) were 0.025 ng/g for AFB1 and AFG1, 0.012 ng/g for AFB2 and AFG2, 0.2 ng/g for OTA, 1.5 ng/g for ZEA, 6.2 ng/g for FB1, FB3 and HT-2 toxin, 9.4 ng/g for FB2 and T-2 toxin, and 18.7 ng/g for DON. In addition, the limits of quantification (LOQ) ranged from 0.04 ng/g for AFB2 and AFG2 to 62 ng/g for DON. The method was successfully applied to the determination of these mycotoxins in 45 cereal samples obtained from the Malaysian market. The results indicated that the method can be applied for the multi-mycotoxin determination of cereals. Taylor and Francis 2011-04-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/33304/1/Soleimany%2C_2011.pdf Soleimany, F. and Jinap, Selamat and Rahmani, Anoshep and Khatib, Alfi (2011) Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system. Food Additives & Contaminants: Part A, 28 (4). pp. 494-501. ISSN 1944-0049 http://www.tandfonline.com/doi/full/10.1080/19440049.2010.551547#.Uqp_DeKuqKI 10.1080/19440049.2010.551547 |
| spellingShingle | QD Chemistry Soleimany, F. Jinap, Selamat Rahmani, Anoshep Khatib, Alfi Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system |
| title | Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system |
| title_full | Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system |
| title_fullStr | Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system |
| title_full_unstemmed | Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system |
| title_short | Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system |
| title_sort | simultaneous detection of 12 mycotoxins in cereals using rp-hplc-pda-fld with phred and a post-column derivatization system |
| topic | QD Chemistry |
| url | http://irep.iium.edu.my/33304/ http://irep.iium.edu.my/33304/ http://irep.iium.edu.my/33304/ http://irep.iium.edu.my/33304/1/Soleimany%2C_2011.pdf |