Optimization of media composition for the production of bioprotein from pineapple skins by liquid-state bioconversion

Two-steps optimization strategy based on statistical experimental design was carried out to enhance bioprotein production by liquid state bioconversion utilizing pineapple skins as substrate. A two-level Plackett-Burman design was employed first where 11 variables were studied for their influence on...

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Main Authors: Jamal, Parveen, Tompang, Mohd Fahrurrazi, Alam, Md. Zahangir
Format: Article
Language:English
Published: Asian Network for Scientific Information 2009
Subjects:
Online Access:http://irep.iium.edu.my/3249/
http://irep.iium.edu.my/3249/1/Biooprotein.pdf
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author Jamal, Parveen
Tompang, Mohd Fahrurrazi
Alam, Md. Zahangir
author_facet Jamal, Parveen
Tompang, Mohd Fahrurrazi
Alam, Md. Zahangir
author_sort Jamal, Parveen
building IIUM Repository
collection Online Access
description Two-steps optimization strategy based on statistical experimental design was carried out to enhance bioprotein production by liquid state bioconversion utilizing pineapple skins as substrate. A two-level Plackett-Burman design was employed first where 11 variables were studied for their influence on bioprotein production. Pineapple Skins Soluble (PSS) sugar content, KH2PO4 and NH4H2PO4 were the most significant variables for improving bioprotein production. A three-level Central Composite Design (CCD) was employed for maximizing the bioprotein production. A mathematical model was developed to show the effects of each medium component and their combinatorial interaction on bioprotein production. The optimal medium composition for maximum bioprotein production was PSS content 1.0, KH2PO4 0.1 and NH4H2PO4 0.25. The final experiment was carried out to validate the model, which has shown to produce 514.2 g kg-1 bioprotein on the 5th day of fermentation with a slight increase of 0.02 from the experimental run in CCD. 2009 Asian Network for Scientific Information.
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spelling iium-32492016-02-15T07:13:39Z http://irep.iium.edu.my/3249/ Optimization of media composition for the production of bioprotein from pineapple skins by liquid-state bioconversion Jamal, Parveen Tompang, Mohd Fahrurrazi Alam, Md. Zahangir TP Chemical technology Two-steps optimization strategy based on statistical experimental design was carried out to enhance bioprotein production by liquid state bioconversion utilizing pineapple skins as substrate. A two-level Plackett-Burman design was employed first where 11 variables were studied for their influence on bioprotein production. Pineapple Skins Soluble (PSS) sugar content, KH2PO4 and NH4H2PO4 were the most significant variables for improving bioprotein production. A three-level Central Composite Design (CCD) was employed for maximizing the bioprotein production. A mathematical model was developed to show the effects of each medium component and their combinatorial interaction on bioprotein production. The optimal medium composition for maximum bioprotein production was PSS content 1.0, KH2PO4 0.1 and NH4H2PO4 0.25. The final experiment was carried out to validate the model, which has shown to produce 514.2 g kg-1 bioprotein on the 5th day of fermentation with a slight increase of 0.02 from the experimental run in CCD. 2009 Asian Network for Scientific Information. Asian Network for Scientific Information 2009-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/3249/1/Biooprotein.pdf Jamal, Parveen and Tompang, Mohd Fahrurrazi and Alam, Md. Zahangir (2009) Optimization of media composition for the production of bioprotein from pineapple skins by liquid-state bioconversion. Journal of Applied Sciences, 9 (17). pp. 3104-3109. ISSN 1812-5662 (O), 1812-5654 (P) http://scialert.net/abstract/?doi=jas.2009.3104.3109 10.3923/jas.2009.3104.3109
spellingShingle TP Chemical technology
Jamal, Parveen
Tompang, Mohd Fahrurrazi
Alam, Md. Zahangir
Optimization of media composition for the production of bioprotein from pineapple skins by liquid-state bioconversion
title Optimization of media composition for the production of bioprotein from pineapple skins by liquid-state bioconversion
title_full Optimization of media composition for the production of bioprotein from pineapple skins by liquid-state bioconversion
title_fullStr Optimization of media composition for the production of bioprotein from pineapple skins by liquid-state bioconversion
title_full_unstemmed Optimization of media composition for the production of bioprotein from pineapple skins by liquid-state bioconversion
title_short Optimization of media composition for the production of bioprotein from pineapple skins by liquid-state bioconversion
title_sort optimization of media composition for the production of bioprotein from pineapple skins by liquid-state bioconversion
topic TP Chemical technology
url http://irep.iium.edu.my/3249/
http://irep.iium.edu.my/3249/
http://irep.iium.edu.my/3249/
http://irep.iium.edu.my/3249/1/Biooprotein.pdf