Kinetic studies on recombinant stem bromelain
Stem bromelain is a plant thiol protease with several industrial and therapeutic applications. This current work presents kinetic studies of recombinant bromelain (recBM) expressed in Escherichia coli BL21-AI on foursynthetic substrates, N-α-carbobenzoxy-L-alanyl-p-nitrophenylester (ZANPE), N-α-carb...
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SciRes.
2013
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| Online Access: | http://irep.iium.edu.my/32114/ http://irep.iium.edu.my/32114/1/Kinetic_study_of_recombinant_stem_bromelain.pdf |
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| author | Bala, Muntari Mel, Maizirwan Jami, Mohammed Saedi Amid, Azura Salleh, Hamzah Mohd. |
| author_facet | Bala, Muntari Mel, Maizirwan Jami, Mohammed Saedi Amid, Azura Salleh, Hamzah Mohd. |
| author_sort | Bala, Muntari |
| building | IIUM Repository |
| collection | Online Access |
| description | Stem bromelain is a plant thiol protease with several industrial and therapeutic applications. This current work presents kinetic studies of recombinant bromelain (recBM) expressed in Escherichia coli BL21-AI on foursynthetic substrates, N-α-carbobenzoxy-L-alanyl-p-nitrophenylester (ZANPE), N-α-carbobenzoxy-L-arginyl-L-ar-ginine-p-nitroanilide (ZAANA), N-α-carbobenzo-xy-L-phenylalanyl-L-valyl-L-arginine-p-nitroanili-de (ZPVANA) and L-pyroglutamyl-L-phenylalanyl-L-leucine-p-nitroanilide (PFLNA). Hydrolytic activities of recBM at various pH and temperature conditions were compared to that of commercial bromelain (cBM). Both enzymes demonstrated high activities at 45o C and pH 5 - 8 for recBM and pH 6 - 8 for cBM. recBM showed marginally lower Kmand slightly higher kcat/Kmfor ZAANA, ZANPE and ZPVANA in comparison to cBM.trans Epoxysuccinyl-L-leucylamido {4- guanidino}butane (E-64) severely affected recBM and cBM hydrolysis of the synthetic substrates by competitive inhibition with Kivalues of 3.6 - 5.1 μM and 5.5 - 6.9 μM for recBM and cBM, respectively. The evaluated properties of recBM including temperature and pH optima, substrate specificity and sensitivity to inhibitors or activators, satisfy the requisites required for food industries.
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| first_indexed | 2025-11-14T15:34:40Z |
| format | Article |
| id | iium-32114 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:34:40Z |
| publishDate | 2013 |
| publisher | SciRes. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-321142021-07-14T09:39:46Z http://irep.iium.edu.my/32114/ Kinetic studies on recombinant stem bromelain Bala, Muntari Mel, Maizirwan Jami, Mohammed Saedi Amid, Azura Salleh, Hamzah Mohd. TP248.13 Biotechnology Stem bromelain is a plant thiol protease with several industrial and therapeutic applications. This current work presents kinetic studies of recombinant bromelain (recBM) expressed in Escherichia coli BL21-AI on foursynthetic substrates, N-α-carbobenzoxy-L-alanyl-p-nitrophenylester (ZANPE), N-α-carbobenzoxy-L-arginyl-L-ar-ginine-p-nitroanilide (ZAANA), N-α-carbobenzo-xy-L-phenylalanyl-L-valyl-L-arginine-p-nitroanili-de (ZPVANA) and L-pyroglutamyl-L-phenylalanyl-L-leucine-p-nitroanilide (PFLNA). Hydrolytic activities of recBM at various pH and temperature conditions were compared to that of commercial bromelain (cBM). Both enzymes demonstrated high activities at 45o C and pH 5 - 8 for recBM and pH 6 - 8 for cBM. recBM showed marginally lower Kmand slightly higher kcat/Kmfor ZAANA, ZANPE and ZPVANA in comparison to cBM.trans Epoxysuccinyl-L-leucylamido {4- guanidino}butane (E-64) severely affected recBM and cBM hydrolysis of the synthetic substrates by competitive inhibition with Kivalues of 3.6 - 5.1 μM and 5.5 - 6.9 μM for recBM and cBM, respectively. The evaluated properties of recBM including temperature and pH optima, substrate specificity and sensitivity to inhibitors or activators, satisfy the requisites required for food industries. SciRes. 2013 Article PeerReviewed application/pdf en http://irep.iium.edu.my/32114/1/Kinetic_study_of_recombinant_stem_bromelain.pdf Bala, Muntari and Mel, Maizirwan and Jami, Mohammed Saedi and Amid, Azura and Salleh, Hamzah Mohd. (2013) Kinetic studies on recombinant stem bromelain. Advances in Enzyme Research, 1 (3). pp. 52-60. ISSN 2328-4846 http://www.scirp.org/journal/aer/ DOI: 10.4236/aer.2013.13006 |
| spellingShingle | TP248.13 Biotechnology Bala, Muntari Mel, Maizirwan Jami, Mohammed Saedi Amid, Azura Salleh, Hamzah Mohd. Kinetic studies on recombinant stem bromelain |
| title | Kinetic studies on recombinant stem bromelain |
| title_full | Kinetic studies on recombinant stem bromelain |
| title_fullStr | Kinetic studies on recombinant stem bromelain |
| title_full_unstemmed | Kinetic studies on recombinant stem bromelain |
| title_short | Kinetic studies on recombinant stem bromelain |
| title_sort | kinetic studies on recombinant stem bromelain |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/32114/ http://irep.iium.edu.my/32114/ http://irep.iium.edu.my/32114/ http://irep.iium.edu.my/32114/1/Kinetic_study_of_recombinant_stem_bromelain.pdf |