LC-MS/MS simulatneous determination of itopride hydrochloride and domperidone in human plasma
A rapid, simple, sensitive and specific liquid chromatography–tandem mass spectrometry method was developed and validated for simultaneous quantification of itopride hydrochloride and domperidone in human plasma. Both drugs were extracted by liquid–liquid extraction with ethyl acetate and saturated...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Vieweg Verlag
2009
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/31912/ http://irep.iium.edu.my/31912/1/Paper8.pdf |
| _version_ | 1848780467140034560 |
|---|---|
| author | Bose, Anirbandeep Bhaumik, Uttam Ghosh, Animesh Chatterjee, Bappaditya Chakraborty, Uday Sankar Sarkar, Amlan Kanti Pal, Tapan Kumar |
| author_facet | Bose, Anirbandeep Bhaumik, Uttam Ghosh, Animesh Chatterjee, Bappaditya Chakraborty, Uday Sankar Sarkar, Amlan Kanti Pal, Tapan Kumar |
| author_sort | Bose, Anirbandeep |
| building | IIUM Repository |
| collection | Online Access |
| description | A rapid, simple, sensitive and specific liquid chromatography–tandem mass spectrometry method was developed and validated for simultaneous quantification of itopride hydrochloride and domperidone in human plasma. Both drugs were extracted by liquid–liquid extraction with ethyl acetate and saturated borax solution. The chromatographic separation was performed on a reversed-phase C18 column with a mobile phase of water–methanol (2:98, v/v) containing 0.5% formic acid. The protonated analyte was quantitated in positive ionization by multiple reaction monitoring with a mass spectrometer. The assay exhibited linearity over the concentration range of 3.33–500 ng mL−1 for itopride hydrochloride and 3.33–100 ng mL−1 for domperidone in human plasma. The precursor to product ion transitions of m/z 359.1–72.3 and 426.0–147.2 were used to measure itopride hydrochloride and domperidone respectively. The method was found suitable for the analysis of plasma samples collected during phase 1 pharmacokinetics study of itopride HCl 50 mg and domperidone 20 mg in 12 healthy volunteers after single oral doses of the combination drug. |
| first_indexed | 2025-11-14T15:34:08Z |
| format | Article |
| id | iium-31912 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:34:08Z |
| publishDate | 2009 |
| publisher | Vieweg Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-319122013-09-20T06:04:41Z http://irep.iium.edu.my/31912/ LC-MS/MS simulatneous determination of itopride hydrochloride and domperidone in human plasma Bose, Anirbandeep Bhaumik, Uttam Ghosh, Animesh Chatterjee, Bappaditya Chakraborty, Uday Sankar Sarkar, Amlan Kanti Pal, Tapan Kumar R Medicine (General) A rapid, simple, sensitive and specific liquid chromatography–tandem mass spectrometry method was developed and validated for simultaneous quantification of itopride hydrochloride and domperidone in human plasma. Both drugs were extracted by liquid–liquid extraction with ethyl acetate and saturated borax solution. The chromatographic separation was performed on a reversed-phase C18 column with a mobile phase of water–methanol (2:98, v/v) containing 0.5% formic acid. The protonated analyte was quantitated in positive ionization by multiple reaction monitoring with a mass spectrometer. The assay exhibited linearity over the concentration range of 3.33–500 ng mL−1 for itopride hydrochloride and 3.33–100 ng mL−1 for domperidone in human plasma. The precursor to product ion transitions of m/z 359.1–72.3 and 426.0–147.2 were used to measure itopride hydrochloride and domperidone respectively. The method was found suitable for the analysis of plasma samples collected during phase 1 pharmacokinetics study of itopride HCl 50 mg and domperidone 20 mg in 12 healthy volunteers after single oral doses of the combination drug. Vieweg Verlag 2009 Article PeerReviewed application/pdf en http://irep.iium.edu.my/31912/1/Paper8.pdf Bose, Anirbandeep and Bhaumik, Uttam and Ghosh, Animesh and Chatterjee, Bappaditya and Chakraborty, Uday Sankar and Sarkar, Amlan Kanti and Pal, Tapan Kumar (2009) LC-MS/MS simulatneous determination of itopride hydrochloride and domperidone in human plasma. Chromatographia, 69 (11/12). pp. 1233-1241. ISSN 1612-1112 (O), 0009-5893 (P) http://link.springer.com/article/10.1365%2Fs10337-009-1032-0 10.1365/s10337-009-1032-0 |
| spellingShingle | R Medicine (General) Bose, Anirbandeep Bhaumik, Uttam Ghosh, Animesh Chatterjee, Bappaditya Chakraborty, Uday Sankar Sarkar, Amlan Kanti Pal, Tapan Kumar LC-MS/MS simulatneous determination of itopride hydrochloride and domperidone in human plasma |
| title | LC-MS/MS simulatneous determination of itopride hydrochloride and domperidone in human plasma |
| title_full | LC-MS/MS simulatneous determination of itopride hydrochloride and domperidone in human plasma |
| title_fullStr | LC-MS/MS simulatneous determination of itopride hydrochloride and domperidone in human plasma |
| title_full_unstemmed | LC-MS/MS simulatneous determination of itopride hydrochloride and domperidone in human plasma |
| title_short | LC-MS/MS simulatneous determination of itopride hydrochloride and domperidone in human plasma |
| title_sort | lc-ms/ms simulatneous determination of itopride hydrochloride and domperidone in human plasma |
| topic | R Medicine (General) |
| url | http://irep.iium.edu.my/31912/ http://irep.iium.edu.my/31912/ http://irep.iium.edu.my/31912/ http://irep.iium.edu.my/31912/1/Paper8.pdf |