Presence of allelic loss and PTEN mutations in malignant gliomas from Malay patients
Loss of heterozygosity (LOH) on several loci and mutations on PTEN tumor suppressor gene (10q23.3) occur frequently in sporadic gliomas. We have performed polymerase chain reaction (PCR)-LOH analysis using microsatellite markers and single-stranded conformational polymorphism (SSCP) analysis to dete...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Malaysian Medical Associations
2004
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/31130/ http://irep.iium.edu.my/31130/1/MJM_Malignant_Gliomas.pdf |
| _version_ | 1848780385577598976 |
|---|---|
| author | Zainuddin, Norafiza Jaafar, Hasnan Isa, Mohd. Nizam Abdullah, Jafri Malin |
| author_facet | Zainuddin, Norafiza Jaafar, Hasnan Isa, Mohd. Nizam Abdullah, Jafri Malin |
| author_sort | Zainuddin, Norafiza |
| building | IIUM Repository |
| collection | Online Access |
| description | Loss of heterozygosity (LOH) on several loci and mutations on PTEN tumor suppressor gene (10q23.3) occur frequently in sporadic gliomas. We have performed polymerase chain reaction (PCR)-LOH analysis using microsatellite markers and single-stranded conformational polymorphism (SSCP) analysis to determine the incidence of allelic losses on chromosome 10q, 9p, 17p and 13q and mutations of exons 5, 6 and 8 of the PTEN gene in malignant gliomas. Twelve of 23 (52.2%) malignant glioma cases showed allelic losses whereas 7 of 23 (30.4%) samples showed aberrant band patterns and mutations of the PTEN gene. Four of these cases showed LOH on 10q23 and mutations of the PTEN gene. The data on LOH indicated the involvement of different genes in gliomagenesis whereas mutations of the PTEN gene indicated the role of PTEN tumor suppressor gene in the progression of glioma in Malay population. |
| first_indexed | 2025-11-14T15:32:50Z |
| format | Article |
| id | iium-31130 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:32:50Z |
| publishDate | 2004 |
| publisher | Malaysian Medical Associations |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-311302024-06-19T08:43:10Z http://irep.iium.edu.my/31130/ Presence of allelic loss and PTEN mutations in malignant gliomas from Malay patients Zainuddin, Norafiza Jaafar, Hasnan Isa, Mohd. Nizam Abdullah, Jafri Malin RC254 Neoplasms. Tumors. Oncology (including Cancer) RC321 Neuroscience. Biological psychiatry. Neuropsychiatry Loss of heterozygosity (LOH) on several loci and mutations on PTEN tumor suppressor gene (10q23.3) occur frequently in sporadic gliomas. We have performed polymerase chain reaction (PCR)-LOH analysis using microsatellite markers and single-stranded conformational polymorphism (SSCP) analysis to determine the incidence of allelic losses on chromosome 10q, 9p, 17p and 13q and mutations of exons 5, 6 and 8 of the PTEN gene in malignant gliomas. Twelve of 23 (52.2%) malignant glioma cases showed allelic losses whereas 7 of 23 (30.4%) samples showed aberrant band patterns and mutations of the PTEN gene. Four of these cases showed LOH on 10q23 and mutations of the PTEN gene. The data on LOH indicated the involvement of different genes in gliomagenesis whereas mutations of the PTEN gene indicated the role of PTEN tumor suppressor gene in the progression of glioma in Malay population. Malaysian Medical Associations 2004-10 Article PeerReviewed application/pdf en http://irep.iium.edu.my/31130/1/MJM_Malignant_Gliomas.pdf Zainuddin, Norafiza and Jaafar, Hasnan and Isa, Mohd. Nizam and Abdullah, Jafri Malin (2004) Presence of allelic loss and PTEN mutations in malignant gliomas from Malay patients. Medical Journal of Malaysia, 59 (4). pp. 468-479. ISSN 0300-5283 http://www.mma.org.my/Publications/MedicalJournalofMalaysia/tabid/69/Default.aspx |
| spellingShingle | RC254 Neoplasms. Tumors. Oncology (including Cancer) RC321 Neuroscience. Biological psychiatry. Neuropsychiatry Zainuddin, Norafiza Jaafar, Hasnan Isa, Mohd. Nizam Abdullah, Jafri Malin Presence of allelic loss and PTEN mutations in malignant gliomas from Malay patients |
| title | Presence of allelic loss and PTEN mutations in malignant gliomas from Malay patients |
| title_full | Presence of allelic loss and PTEN mutations in malignant gliomas from Malay patients |
| title_fullStr | Presence of allelic loss and PTEN mutations in malignant gliomas from Malay patients |
| title_full_unstemmed | Presence of allelic loss and PTEN mutations in malignant gliomas from Malay patients |
| title_short | Presence of allelic loss and PTEN mutations in malignant gliomas from Malay patients |
| title_sort | presence of allelic loss and pten mutations in malignant gliomas from malay patients |
| topic | RC254 Neoplasms. Tumors. Oncology (including Cancer) RC321 Neuroscience. Biological psychiatry. Neuropsychiatry |
| url | http://irep.iium.edu.my/31130/ http://irep.iium.edu.my/31130/ http://irep.iium.edu.my/31130/1/MJM_Malignant_Gliomas.pdf |