Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice
Betulinic acid was isolated from methanolic extract by crystallization and chromatography techniques. S.C. methanolic extract was analyzed by UV-Vis spectrophotometry, FTIR, LCMS, and HPLC. Antiangiogenic effect was studied on rat aortic rings, matrigel tube formation, cell proliferation and migra...
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| Format: | Article |
| Language: | English |
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BioMed Central
2013
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| Online Access: | http://irep.iium.edu.my/30791/ http://irep.iium.edu.my/30791/1/BMC-12July_2013-Syzygium_campanulatum.pdf |
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| author | Abdalrahim , FA Aisha Zhari I, Ismail Khalid , M Abu-Saleh Siddiqui, Mohammad Jamshed Ahmad Ghafar, Gheniya Shah, Amin Malik |
| author_facet | Abdalrahim , FA Aisha Zhari I, Ismail Khalid , M Abu-Saleh Siddiqui, Mohammad Jamshed Ahmad Ghafar, Gheniya Shah, Amin Malik |
| author_sort | Abdalrahim , FA Aisha |
| building | IIUM Repository |
| collection | Online Access |
| description | Betulinic acid was isolated from methanolic extract by crystallization and chromatography techniques. S.C. methanolic extract was analyzed by UV-Vis spectrophotometry, FTIR, LCMS, and HPLC. Antiangiogenic effect was studied on rat aortic rings, matrigel tube
formation, cell proliferation and migration, and expression of vascular endothelial growth factor (VEGF). Antitumor effect was studied using a subcutaneous tumor model of HCT 116 colorectal carcinoma cells established in nude mice.
Results
Analysis by HPLC, LC-MS and FTIR confirm presence of betulinic acid in S.C. methanolic extract. Quantitative analysis by HPLC indicates presence of betulinic acid in S.C. extract at 5.42 ± 0.09% (w/w). Antiangiogenesis study showed potent inhibition of microvessels outgrowth in rat aortic rings, and studies on normal and cancer cells did not show any significant cytotoxic effect. Antiangiogenic effect was further confirmed by inhibition of tube
formation on matrigel matrix that involves human endothelial cells (IC50 = 17.6 ± 2.9 μg/ml).
S.C. extract also inhibited migration of endothelial cells and suppressed expression of VEGF. In vivo antiangiogenic study showed inhibition of new blood vessels in chicken embryo chorioallantoic membrane (CAM), and in vivo antitumor study showed significant inhibition of tumor growth due to reduction of intratumor blood vessels and induction of cell death.
Conclusion
Collectively, our results indicate S. campanulatum as antiangiogenic and antitumor candidate, and a new source of betulinic acid. |
| first_indexed | 2025-11-14T15:32:09Z |
| format | Article |
| id | iium-30791 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:32:09Z |
| publishDate | 2013 |
| publisher | BioMed Central |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-307912013-08-03T07:08:05Z http://irep.iium.edu.my/30791/ Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice Abdalrahim , FA Aisha Zhari I, Ismail Khalid , M Abu-Saleh Siddiqui, Mohammad Jamshed Ahmad Ghafar, Gheniya Shah, Amin Malik QD Chemistry Betulinic acid was isolated from methanolic extract by crystallization and chromatography techniques. S.C. methanolic extract was analyzed by UV-Vis spectrophotometry, FTIR, LCMS, and HPLC. Antiangiogenic effect was studied on rat aortic rings, matrigel tube formation, cell proliferation and migration, and expression of vascular endothelial growth factor (VEGF). Antitumor effect was studied using a subcutaneous tumor model of HCT 116 colorectal carcinoma cells established in nude mice. Results Analysis by HPLC, LC-MS and FTIR confirm presence of betulinic acid in S.C. methanolic extract. Quantitative analysis by HPLC indicates presence of betulinic acid in S.C. extract at 5.42 ± 0.09% (w/w). Antiangiogenesis study showed potent inhibition of microvessels outgrowth in rat aortic rings, and studies on normal and cancer cells did not show any significant cytotoxic effect. Antiangiogenic effect was further confirmed by inhibition of tube formation on matrigel matrix that involves human endothelial cells (IC50 = 17.6 ± 2.9 μg/ml). S.C. extract also inhibited migration of endothelial cells and suppressed expression of VEGF. In vivo antiangiogenic study showed inhibition of new blood vessels in chicken embryo chorioallantoic membrane (CAM), and in vivo antitumor study showed significant inhibition of tumor growth due to reduction of intratumor blood vessels and induction of cell death. Conclusion Collectively, our results indicate S. campanulatum as antiangiogenic and antitumor candidate, and a new source of betulinic acid. BioMed Central 2013-07-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/30791/1/BMC-12July_2013-Syzygium_campanulatum.pdf Abdalrahim , FA Aisha and Zhari I, Ismail and Khalid , M Abu-Saleh and Siddiqui, Mohammad Jamshed Ahmad and Ghafar, Gheniya and Shah, Amin Malik (2013) Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice. BMC Complementary and Alternative Medicine (13). p. 168. ISSN 1472-6882 http://www.biomedcentral.com/1472-6882/13/168 |
| spellingShingle | QD Chemistry Abdalrahim , FA Aisha Zhari I, Ismail Khalid , M Abu-Saleh Siddiqui, Mohammad Jamshed Ahmad Ghafar, Gheniya Shah, Amin Malik Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice |
| title | Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice |
| title_full | Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice |
| title_fullStr | Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice |
| title_full_unstemmed | Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice |
| title_short | Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice |
| title_sort | syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice |
| topic | QD Chemistry |
| url | http://irep.iium.edu.my/30791/ http://irep.iium.edu.my/30791/ http://irep.iium.edu.my/30791/1/BMC-12July_2013-Syzygium_campanulatum.pdf |