Interaction between phosphoprotein and nucleocapsid of newcastle disease virus
The phosphoprotein (P) of paramyxoviruses plays a central role in the viral genome RNA replication and transcription. In genome replication, the P binds to the unassembled nucleocapsid (NP0) and activates it to encapsidate the newly synthesized viral genomic RNA. P also plays a supplemental role...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2009
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/29281/ http://irep.iium.edu.my/29281/5/Interaction_Between_Phosphoprotein.pdf |
| _version_ | 1848780093634117632 |
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| author | Ahmad Raus, Raha Tan, Wen Siang Ali, Abdul Manaf Yusoff, Khatijah |
| author_facet | Ahmad Raus, Raha Tan, Wen Siang Ali, Abdul Manaf Yusoff, Khatijah |
| author_sort | Ahmad Raus, Raha |
| building | IIUM Repository |
| collection | Online Access |
| description | The phosphoprotein (P) of paramyxoviruses plays a central role in the viral genome RNA
replication and transcription. In genome replication, the P binds to the unassembled
nucleocapsid (NP0) and activates it to encapsidate the newly synthesized viral genomic
RNA. P also plays a supplemental role in genomic RNA synthesis by binding to the
ribonucleoprotein template which composed of genomic RNA in complex with the NP.
In this study, the P region of Newcastle disease virus (NDV) that binds to assembled NP
(NPNC) was mapped. A total of 18 N- and C-terminally truncated P mutants were
synthesized by in vitro translation in rabbit reticulocytes containing [35S]-methionine.
These radiolabelled mutants were mixed with the purified full-length NP and determined
their interactions in immunoprecipitation assay and protein binding assay.
Immunoprecipitation assay showed that the region of P that binds to NPNC is located
within the internal region of C-terminal half of P, from amino acids 243 to 279. Protein
binding assay also showed a similar interactive domain except that the interactive region
is slightly bigger (amino acids 224-279). Nevertheless, both assays showed that the Nterminal
half and immediate C-terminal end of P is not involved in the binding of P to
NPNC. |
| first_indexed | 2025-11-14T15:28:11Z |
| format | Proceeding Paper |
| id | iium-29281 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:28:11Z |
| publishDate | 2009 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-292812013-06-25T11:17:26Z http://irep.iium.edu.my/29281/ Interaction between phosphoprotein and nucleocapsid of newcastle disease virus Ahmad Raus, Raha Tan, Wen Siang Ali, Abdul Manaf Yusoff, Khatijah Q Science (General) QR355 Virology The phosphoprotein (P) of paramyxoviruses plays a central role in the viral genome RNA replication and transcription. In genome replication, the P binds to the unassembled nucleocapsid (NP0) and activates it to encapsidate the newly synthesized viral genomic RNA. P also plays a supplemental role in genomic RNA synthesis by binding to the ribonucleoprotein template which composed of genomic RNA in complex with the NP. In this study, the P region of Newcastle disease virus (NDV) that binds to assembled NP (NPNC) was mapped. A total of 18 N- and C-terminally truncated P mutants were synthesized by in vitro translation in rabbit reticulocytes containing [35S]-methionine. These radiolabelled mutants were mixed with the purified full-length NP and determined their interactions in immunoprecipitation assay and protein binding assay. Immunoprecipitation assay showed that the region of P that binds to NPNC is located within the internal region of C-terminal half of P, from amino acids 243 to 279. Protein binding assay also showed a similar interactive domain except that the interactive region is slightly bigger (amino acids 224-279). Nevertheless, both assays showed that the Nterminal half and immediate C-terminal end of P is not involved in the binding of P to NPNC. 2009 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/29281/5/Interaction_Between_Phosphoprotein.pdf Ahmad Raus, Raha and Tan, Wen Siang and Ali, Abdul Manaf and Yusoff, Khatijah (2009) Interaction between phosphoprotein and nucleocapsid of newcastle disease virus. In: Malaysian International Conference on Trends in Bioprocess Engineering., 12 - 13 December 2009, Kuala Lumpur. |
| spellingShingle | Q Science (General) QR355 Virology Ahmad Raus, Raha Tan, Wen Siang Ali, Abdul Manaf Yusoff, Khatijah Interaction between phosphoprotein and nucleocapsid of newcastle disease virus |
| title | Interaction between phosphoprotein and nucleocapsid of newcastle disease virus |
| title_full | Interaction between phosphoprotein and nucleocapsid of newcastle disease virus |
| title_fullStr | Interaction between phosphoprotein and nucleocapsid of newcastle disease virus |
| title_full_unstemmed | Interaction between phosphoprotein and nucleocapsid of newcastle disease virus |
| title_short | Interaction between phosphoprotein and nucleocapsid of newcastle disease virus |
| title_sort | interaction between phosphoprotein and nucleocapsid of newcastle disease virus |
| topic | Q Science (General) QR355 Virology |
| url | http://irep.iium.edu.my/29281/ http://irep.iium.edu.my/29281/5/Interaction_Between_Phosphoprotein.pdf |