Palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet
Oxidative stress contributes to cardiovascular diseases. We aimed to study the effects of palm tocotrienol-rich fraction (TRF) on plasma homocysteine and cardiac oxidative stress in rats fed with a high-methionine diet. Forty-two male Wistar rats were divided into six groups. The first group was the...
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| Language: | English |
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Springer Netherlands
2013
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| Online Access: | http://irep.iium.edu.my/29200/ http://irep.iium.edu.my/29200/1/K.-Z._Norsidah_et_al_2013.pdf |
| _version_ | 1848780079707979776 |
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| author | Ku Zaifah, Norsidah Asmadi, Ahmad Yusof Azizi, Ayob Othman, Faizah Kamisah, Yusof |
| author_facet | Ku Zaifah, Norsidah Asmadi, Ahmad Yusof Azizi, Ayob Othman, Faizah Kamisah, Yusof |
| author_sort | Ku Zaifah, Norsidah |
| building | IIUM Repository |
| collection | Online Access |
| description | Oxidative stress contributes to cardiovascular diseases. We aimed to study the effects of palm tocotrienol-rich fraction (TRF) on plasma homocysteine and cardiac oxidative stress in rats fed with a high-methionine diet. Forty-two male Wistar rats were divided into six groups. The first group was the control. Groups 2-6 were fed 1 % methionine diet for 10 weeks. From week 6 onward, folate (8 mg/kg diet) or palm TRF (30, 60 and 150 mg/kg diet) was added into the diet of groups 3, 4, 5 and 6. The rats were then killed. Palm TRF at 150 mg/kg and folate supplementation prevented the increase in plasma total homocysteine (4.14 ± 0.33 and 4.30 ± 0.26 vs 5.49 ± 0.25 mmol/L, p < 0.05) induced by a high-methionine diet. The increased heart thiobarbituric acid reactive substance in rats fed with high-methionine diet was also prevented by the supplementations of palm TRF (60 and 150 mg/kg) and folate. The high-methionine group had a lower glutathione peroxidase activity (49 ± 3 vs 69 ± 4 pmol/mg protein/min) than the control group. This reduction was reversed by palm TRF at 60 and 150 mg/kg diet (p < 0.05), but not by folate. Catalase and superoxide dismutase activities were unaffected by both methionine and vitamin supplementations. In conclusion, palm TRF was comparable to folate in reducing high-methionine diet-induced hyperhomocysteinemia and oxidative stress in the rats' hearts. However, palm TRF was more effective than folate in preserving the heart glutathione peroxidase enzyme activity. |
| first_indexed | 2025-11-14T15:27:58Z |
| format | Article |
| id | iium-29200 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:27:58Z |
| publishDate | 2013 |
| publisher | Springer Netherlands |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-292002015-04-07T03:15:05Z http://irep.iium.edu.my/29200/ Palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet Ku Zaifah, Norsidah Asmadi, Ahmad Yusof Azizi, Ayob Othman, Faizah Kamisah, Yusof R Medicine (General) Oxidative stress contributes to cardiovascular diseases. We aimed to study the effects of palm tocotrienol-rich fraction (TRF) on plasma homocysteine and cardiac oxidative stress in rats fed with a high-methionine diet. Forty-two male Wistar rats were divided into six groups. The first group was the control. Groups 2-6 were fed 1 % methionine diet for 10 weeks. From week 6 onward, folate (8 mg/kg diet) or palm TRF (30, 60 and 150 mg/kg diet) was added into the diet of groups 3, 4, 5 and 6. The rats were then killed. Palm TRF at 150 mg/kg and folate supplementation prevented the increase in plasma total homocysteine (4.14 ± 0.33 and 4.30 ± 0.26 vs 5.49 ± 0.25 mmol/L, p < 0.05) induced by a high-methionine diet. The increased heart thiobarbituric acid reactive substance in rats fed with high-methionine diet was also prevented by the supplementations of palm TRF (60 and 150 mg/kg) and folate. The high-methionine group had a lower glutathione peroxidase activity (49 ± 3 vs 69 ± 4 pmol/mg protein/min) than the control group. This reduction was reversed by palm TRF at 60 and 150 mg/kg diet (p < 0.05), but not by folate. Catalase and superoxide dismutase activities were unaffected by both methionine and vitamin supplementations. In conclusion, palm TRF was comparable to folate in reducing high-methionine diet-induced hyperhomocysteinemia and oxidative stress in the rats' hearts. However, palm TRF was more effective than folate in preserving the heart glutathione peroxidase enzyme activity. Springer Netherlands 2013-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/29200/1/K.-Z._Norsidah_et_al_2013.pdf Ku Zaifah, Norsidah and Asmadi, Ahmad Yusof and Azizi, Ayob and Othman, Faizah and Kamisah, Yusof (2013) Palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet. Journal of Physiology and Biochemistry, 69 (3). pp. 441-449. ISSN 1138-7548 (In Press) http://link.springer.com/article/10.1007%2Fs13105-012-0226-3 10.1007/s13105-012-0226-3 |
| spellingShingle | R Medicine (General) Ku Zaifah, Norsidah Asmadi, Ahmad Yusof Azizi, Ayob Othman, Faizah Kamisah, Yusof Palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet |
| title | Palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet |
| title_full | Palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet |
| title_fullStr | Palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet |
| title_full_unstemmed | Palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet |
| title_short | Palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet |
| title_sort | palm tocotrienol-rich fraction reduced plasma homocysteine and heart oxidative stress in rats fed with a high-methionine diet |
| topic | R Medicine (General) |
| url | http://irep.iium.edu.my/29200/ http://irep.iium.edu.my/29200/ http://irep.iium.edu.my/29200/ http://irep.iium.edu.my/29200/1/K.-Z._Norsidah_et_al_2013.pdf |