Study on the cytotoxicity and chromosome aberration following implantation of sea coral in rabbits
Coral has been used as a bone substitute in many experimental studies. It has been proven to be biocompatible, biodegradable and easy to handle: and it has not been found to cause any inflammatory responses. The present study was undertaken to determine the cytotoxicity in terms of mitotic index as...
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| Format: | Article |
| Language: | English |
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Airlangga University Press
2006
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| Online Access: | http://irep.iium.edu.my/29053/ http://irep.iium.edu.my/29053/2/DENTJ-39-1-02.pdf |
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| author | TP., Kannan Freeda Woon Faiza Woon, Tai Keat Witjaksono, Widowati Abdul Rani, Samsudin |
| author_facet | TP., Kannan Freeda Woon Faiza Woon, Tai Keat Witjaksono, Widowati Abdul Rani, Samsudin |
| author_sort | TP., Kannan |
| building | IIUM Repository |
| collection | Online Access |
| description | Coral has been used as a bone substitute in many experimental studies. It has been proven to be biocompatible, biodegradable and easy to handle: and it has not been found to cause any inflammatory responses. The present study was undertaken to determine the cytotoxicity in terms of mitotic index as well as the clastogenic effect (chromosome aberration) of sea coral implantation in
rabbits. The animals comprised of five male adult healthy New Zealand White (Oryctolagus cuniculus) rabbits. The biomaterial, sea coral granules used in this study was obtained from Porites species and processed by the tissue bank of Universiti Sains Malaysia,Health Campus, Kubang Kerian, Malaysia. The blood samples were collected twice from the rabbits, once before the implantation of
the sea coral granules (which acted as the control) and the other, one week after the implantation (which acted as the treatment) and lymphocyte cultures were set up. The cultures were then harvested and the chromosomes were prepared for analysis. The diploid number of chromosomes in the rabbits (Oryctolagus cuniculus) was found to be 44. Mean mitotic indices of 3.84 ± 0.54 per cent and 3.76 ± 0.23 per cent were obtained before and after implantation of sea coral granules respectively. There were no structural or numerical chromosomal aberrations observed in both the cases. The mitotic index values and chromosomal analyses in this preliminary study carried out indicate that the biomaterial, sea coral granules is non-cytotoxic and non-clastogenic under the present test conditions |
| first_indexed | 2025-11-14T15:27:36Z |
| format | Article |
| id | iium-29053 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:27:36Z |
| publishDate | 2006 |
| publisher | Airlangga University Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-290532013-04-26T02:44:37Z http://irep.iium.edu.my/29053/ Study on the cytotoxicity and chromosome aberration following implantation of sea coral in rabbits TP., Kannan Freeda Woon Faiza Woon, Tai Keat Witjaksono, Widowati Abdul Rani, Samsudin RK Dentistry Coral has been used as a bone substitute in many experimental studies. It has been proven to be biocompatible, biodegradable and easy to handle: and it has not been found to cause any inflammatory responses. The present study was undertaken to determine the cytotoxicity in terms of mitotic index as well as the clastogenic effect (chromosome aberration) of sea coral implantation in rabbits. The animals comprised of five male adult healthy New Zealand White (Oryctolagus cuniculus) rabbits. The biomaterial, sea coral granules used in this study was obtained from Porites species and processed by the tissue bank of Universiti Sains Malaysia,Health Campus, Kubang Kerian, Malaysia. The blood samples were collected twice from the rabbits, once before the implantation of the sea coral granules (which acted as the control) and the other, one week after the implantation (which acted as the treatment) and lymphocyte cultures were set up. The cultures were then harvested and the chromosomes were prepared for analysis. The diploid number of chromosomes in the rabbits (Oryctolagus cuniculus) was found to be 44. Mean mitotic indices of 3.84 ± 0.54 per cent and 3.76 ± 0.23 per cent were obtained before and after implantation of sea coral granules respectively. There were no structural or numerical chromosomal aberrations observed in both the cases. The mitotic index values and chromosomal analyses in this preliminary study carried out indicate that the biomaterial, sea coral granules is non-cytotoxic and non-clastogenic under the present test conditions Airlangga University Press 2006 Article PeerReviewed application/pdf en http://irep.iium.edu.my/29053/2/DENTJ-39-1-02.pdf TP., Kannan and Freeda Woon Faiza Woon, Tai Keat and Witjaksono, Widowati and Abdul Rani, Samsudin (2006) Study on the cytotoxicity and chromosome aberration following implantation of sea coral in rabbits. Dental Journal, 39 (1). pp. 8-11. ISSN 0852-9027 |
| spellingShingle | RK Dentistry TP., Kannan Freeda Woon Faiza Woon, Tai Keat Witjaksono, Widowati Abdul Rani, Samsudin Study on the cytotoxicity and chromosome aberration following implantation of sea coral in rabbits |
| title | Study on the cytotoxicity and chromosome aberration following implantation of sea coral in rabbits |
| title_full | Study on the cytotoxicity and chromosome aberration following implantation of sea coral in rabbits |
| title_fullStr | Study on the cytotoxicity and chromosome aberration following implantation of sea coral in rabbits |
| title_full_unstemmed | Study on the cytotoxicity and chromosome aberration following implantation of sea coral in rabbits |
| title_short | Study on the cytotoxicity and chromosome aberration following implantation of sea coral in rabbits |
| title_sort | study on the cytotoxicity and chromosome aberration following implantation of sea coral in rabbits |
| topic | RK Dentistry |
| url | http://irep.iium.edu.my/29053/ http://irep.iium.edu.my/29053/2/DENTJ-39-1-02.pdf |