Focused ion beam machining of cell culture dish
Cell culture is one of the major tools in life sciences. In regular cell culture, cells are placed into flat petri dishes made of glass or plastic. As the cells are strongly adhered to the dish, an enzyme treatment is applied to stripe them. In case of nanopillar dish, the cells contact the heads...
| Main Author: | |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2008
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/28914/ http://irep.iium.edu.my/28914/1/088_AiT_Bulletine_2008_4_%289%29.pdf |
| _version_ | 1848780032530448384 |
|---|---|
| author | Ali, Mohammad Yeakub |
| author_facet | Ali, Mohammad Yeakub |
| author_sort | Ali, Mohammad Yeakub |
| building | IIUM Repository |
| collection | Online Access |
| description | Cell culture is one of the major tools in life sciences. In regular cell culture, cells are placed
into flat petri dishes made of glass or plastic. As the cells are strongly adhered to the dish, an enzyme
treatment is applied to stripe them. In case of nanopillar dish, the cells contact the heads of the pillars
then multiply by division supported on top of the nanopillars. The nanopillar structure enabled easy
subculture of the cells without conventional trypsinization where the cultured cells can be collected
simply by pipetting. However, it remains a challenge to fabricate high aspect ratio metallic or polymeric
nanopillars of few hundred nanometer of diameter.
This research presents focused ion beam (FIB) maskless sputtering technique for the fabrication
of nanopillars sheet to be used in cell culture dish. Nanopore sheet on metallic materials is also
machined using FIB which can be used as a master microtool for the mass production of polymer
nanopillars sheets by nanoprinting. The typical diameter and height of the nanopillars are 400 nm and
2 ?m respectively. |
| first_indexed | 2025-11-14T15:27:13Z |
| format | Proceeding Paper |
| id | iium-28914 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:27:13Z |
| publishDate | 2008 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-289142018-12-05T08:30:49Z http://irep.iium.edu.my/28914/ Focused ion beam machining of cell culture dish Ali, Mohammad Yeakub T Technology (General) TJ Mechanical engineering and machinery TS Manufactures Cell culture is one of the major tools in life sciences. In regular cell culture, cells are placed into flat petri dishes made of glass or plastic. As the cells are strongly adhered to the dish, an enzyme treatment is applied to stripe them. In case of nanopillar dish, the cells contact the heads of the pillars then multiply by division supported on top of the nanopillars. The nanopillar structure enabled easy subculture of the cells without conventional trypsinization where the cultured cells can be collected simply by pipetting. However, it remains a challenge to fabricate high aspect ratio metallic or polymeric nanopillars of few hundred nanometer of diameter. This research presents focused ion beam (FIB) maskless sputtering technique for the fabrication of nanopillars sheet to be used in cell culture dish. Nanopore sheet on metallic materials is also machined using FIB which can be used as a master microtool for the mass production of polymer nanopillars sheets by nanoprinting. The typical diameter and height of the nanopillars are 400 nm and 2 ?m respectively. 2008 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/28914/1/088_AiT_Bulletine_2008_4_%289%29.pdf Ali, Mohammad Yeakub (2008) Focused ion beam machining of cell culture dish. In: ISE Seminar 2008, 3rd March 2008, Thailand. (Unpublished) |
| spellingShingle | T Technology (General) TJ Mechanical engineering and machinery TS Manufactures Ali, Mohammad Yeakub Focused ion beam machining of cell culture dish |
| title | Focused ion beam machining of cell culture dish |
| title_full | Focused ion beam machining of cell culture dish |
| title_fullStr | Focused ion beam machining of cell culture dish |
| title_full_unstemmed | Focused ion beam machining of cell culture dish |
| title_short | Focused ion beam machining of cell culture dish |
| title_sort | focused ion beam machining of cell culture dish |
| topic | T Technology (General) TJ Mechanical engineering and machinery TS Manufactures |
| url | http://irep.iium.edu.my/28914/ http://irep.iium.edu.my/28914/1/088_AiT_Bulletine_2008_4_%289%29.pdf |