Antioxidant activity of winter melon (Benincasa Hispida) seeds using conventional soxhlet extraction technique

In this study, the effect of different solvent including ethanol, n-hexane and ethyl acetate on antioxidant activity and total phenolic content (TPC) of winter melon (Benincasa hispida) seeds extract was investigated using conventional Soxhlet extraction (CSE). DPPH and ABTS scavenging activity an...

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Main Authors: B., Mandana, Abdul Rahman, Russly, S. T., Farah, Mohd Adzahan, Noranizan, Sarker, Md. Zaidul Islam, Ganjloo, Ali
Format: Article
Language:English
Published: University Putra Malaysia 2012
Subjects:
Online Access:http://irep.iium.edu.my/27855/
http://irep.iium.edu.my/27855/1/%2830%29IFRJ-2011-161_Mandana.pdf
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author B., Mandana
Abdul Rahman, Russly
S. T., Farah
Mohd Adzahan, Noranizan
Sarker, Md. Zaidul Islam
Ganjloo, Ali
author_facet B., Mandana
Abdul Rahman, Russly
S. T., Farah
Mohd Adzahan, Noranizan
Sarker, Md. Zaidul Islam
Ganjloo, Ali
author_sort B., Mandana
building IIUM Repository
collection Online Access
description In this study, the effect of different solvent including ethanol, n-hexane and ethyl acetate on antioxidant activity and total phenolic content (TPC) of winter melon (Benincasa hispida) seeds extract was investigated using conventional Soxhlet extraction (CSE). DPPH and ABTS scavenging activity and TPC results indicated that the seed extracts obtained using ethanol possessed the highest antioxidant activity and followed by ethyl acetate and n-hexane. By considering obtained results, it was clear that there was a high positive correlation between TPC and antioxidant activity. Linoleic acid forms a significant percentage of unsaturated fatty acids of the seed extract (60.6%). It is well known that essential fatty acids including linoleic acid and linolenic acid which are detected in extracts play important roles in preventing many disease and abnormal differentiation problems. B. hispida seeds are potential source of natural antioxidant compounds to replace synthetic antioxidants.
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spelling iium-278552013-02-13T23:39:13Z http://irep.iium.edu.my/27855/ Antioxidant activity of winter melon (Benincasa Hispida) seeds using conventional soxhlet extraction technique B., Mandana Abdul Rahman, Russly S. T., Farah Mohd Adzahan, Noranizan Sarker, Md. Zaidul Islam Ganjloo, Ali QD Chemistry In this study, the effect of different solvent including ethanol, n-hexane and ethyl acetate on antioxidant activity and total phenolic content (TPC) of winter melon (Benincasa hispida) seeds extract was investigated using conventional Soxhlet extraction (CSE). DPPH and ABTS scavenging activity and TPC results indicated that the seed extracts obtained using ethanol possessed the highest antioxidant activity and followed by ethyl acetate and n-hexane. By considering obtained results, it was clear that there was a high positive correlation between TPC and antioxidant activity. Linoleic acid forms a significant percentage of unsaturated fatty acids of the seed extract (60.6%). It is well known that essential fatty acids including linoleic acid and linolenic acid which are detected in extracts play important roles in preventing many disease and abnormal differentiation problems. B. hispida seeds are potential source of natural antioxidant compounds to replace synthetic antioxidants. University Putra Malaysia 2012 Article PeerReviewed application/pdf en cc_by_nd http://irep.iium.edu.my/27855/1/%2830%29IFRJ-2011-161_Mandana.pdf B., Mandana and Abdul Rahman, Russly and S. T., Farah and Mohd Adzahan, Noranizan and Sarker, Md. Zaidul Islam and Ganjloo, Ali (2012) Antioxidant activity of winter melon (Benincasa Hispida) seeds using conventional soxhlet extraction technique. International Food Research Journal, 19 (1). pp. 229-234. ISSN ISSN: 1985-4668
spellingShingle QD Chemistry
B., Mandana
Abdul Rahman, Russly
S. T., Farah
Mohd Adzahan, Noranizan
Sarker, Md. Zaidul Islam
Ganjloo, Ali
Antioxidant activity of winter melon (Benincasa Hispida) seeds using conventional soxhlet extraction technique
title Antioxidant activity of winter melon (Benincasa Hispida) seeds using conventional soxhlet extraction technique
title_full Antioxidant activity of winter melon (Benincasa Hispida) seeds using conventional soxhlet extraction technique
title_fullStr Antioxidant activity of winter melon (Benincasa Hispida) seeds using conventional soxhlet extraction technique
title_full_unstemmed Antioxidant activity of winter melon (Benincasa Hispida) seeds using conventional soxhlet extraction technique
title_short Antioxidant activity of winter melon (Benincasa Hispida) seeds using conventional soxhlet extraction technique
title_sort antioxidant activity of winter melon (benincasa hispida) seeds using conventional soxhlet extraction technique
topic QD Chemistry
url http://irep.iium.edu.my/27855/
http://irep.iium.edu.my/27855/1/%2830%29IFRJ-2011-161_Mandana.pdf