A rapid method for large sample number extraction of proteins from mammalian cells
Finding the best method of cell lysis and extraction of protein is the key step in detection and identification of proteins in all applications of proteomics. The methods currently used for protein extraction from mammalian cells are usually laborious, expensive or insufficiently reliable. R...
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| Format: | Article |
| Language: | English |
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Tissue Engineering and Regenerative Medicine Society of Malaysia
2012
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| Online Access: | http://irep.iium.edu.my/27821/ http://irep.iium.edu.my/27821/1/Paper_-_Packeer-Mohamed_et_al_2012_%28RegRes%29.pdf |
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| author | Packeer Mohamed, Vasila Hashim, Yumi Zuhanis Has-Yun Amid, Azura Mohd Salleh, Hamzah Yusof, Faridah Mel, Maizirwan |
| author_facet | Packeer Mohamed, Vasila Hashim, Yumi Zuhanis Has-Yun Amid, Azura Mohd Salleh, Hamzah Yusof, Faridah Mel, Maizirwan |
| author_sort | Packeer Mohamed, Vasila |
| building | IIUM Repository |
| collection | Online Access |
| description | Finding the best method of cell lysis and extraction of protein is the key step in detection
and identification of proteins in all applications of proteomics. The methods currently
used for protein extraction from mammalian cells are usually laborious, expensive or
insufficiently reliable. Recently, researchers working with mammalian cells conduct
protein extraction widely and frequently. This frequent dealing with a large number of
samples becomes tedious work. Even though there are many commercial lysis buffers on
the market, they are usually expensive. Therefore, in this study, protein extraction lysis
buffer was designed to provide highly efficient total protein extraction from cultured
mammalian cells. The simple and unique composition of this lysis buffer containing
sodium dodecyl sulphate (SDS) dissolves cell membranes and extracts total cellular
protein in only 45 minutes. The novel extraction method and lysis buffer composition
was formulated using classical optimization or one factor at a time (OFAT) study. In this
method, cell pellet from mammalian cells (CHO-K1) were subjected to different lysis
buffer treatment procedure and then incubated at room temperature. The SDS-PAGE was
performed to confirm the proteins extracted. The method was extended to bacteria
(Escherichia coli, BL21-AI), yeast (Pichia pastoris) and repeated for mammalian (CHOK1) for confirmation. It yields complete extraction for bacteria and mammalian cells. As
such, this lysis buffer when used for preparing protein extracts from bacteria and
mammalian cells offers rapid, reliable and cheap protocol which is applicable to routine
laboratory analysis especially for protein estimation and SDS-PAGE. |
| first_indexed | 2025-11-14T15:24:42Z |
| format | Article |
| id | iium-27821 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:24:42Z |
| publishDate | 2012 |
| publisher | Tissue Engineering and Regenerative Medicine Society of Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-278212013-02-14T00:11:25Z http://irep.iium.edu.my/27821/ A rapid method for large sample number extraction of proteins from mammalian cells Packeer Mohamed, Vasila Hashim, Yumi Zuhanis Has-Yun Amid, Azura Mohd Salleh, Hamzah Yusof, Faridah Mel, Maizirwan Q Science (General) Finding the best method of cell lysis and extraction of protein is the key step in detection and identification of proteins in all applications of proteomics. The methods currently used for protein extraction from mammalian cells are usually laborious, expensive or insufficiently reliable. Recently, researchers working with mammalian cells conduct protein extraction widely and frequently. This frequent dealing with a large number of samples becomes tedious work. Even though there are many commercial lysis buffers on the market, they are usually expensive. Therefore, in this study, protein extraction lysis buffer was designed to provide highly efficient total protein extraction from cultured mammalian cells. The simple and unique composition of this lysis buffer containing sodium dodecyl sulphate (SDS) dissolves cell membranes and extracts total cellular protein in only 45 minutes. The novel extraction method and lysis buffer composition was formulated using classical optimization or one factor at a time (OFAT) study. In this method, cell pellet from mammalian cells (CHO-K1) were subjected to different lysis buffer treatment procedure and then incubated at room temperature. The SDS-PAGE was performed to confirm the proteins extracted. The method was extended to bacteria (Escherichia coli, BL21-AI), yeast (Pichia pastoris) and repeated for mammalian (CHOK1) for confirmation. It yields complete extraction for bacteria and mammalian cells. As such, this lysis buffer when used for preparing protein extracts from bacteria and mammalian cells offers rapid, reliable and cheap protocol which is applicable to routine laboratory analysis especially for protein estimation and SDS-PAGE. Tissue Engineering and Regenerative Medicine Society of Malaysia 2012-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/27821/1/Paper_-_Packeer-Mohamed_et_al_2012_%28RegRes%29.pdf Packeer Mohamed, Vasila and Hashim, Yumi Zuhanis Has-Yun and Amid, Azura and Mohd Salleh, Hamzah and Yusof, Faridah and Mel, Maizirwan (2012) A rapid method for large sample number extraction of proteins from mammalian cells. Regenerative Research, 1 (2). pp. 49-55. ISSN 2232-0822 (Online) http://regres.tesma.org.my/pdf/RR-151012-006%288%29.pdf |
| spellingShingle | Q Science (General) Packeer Mohamed, Vasila Hashim, Yumi Zuhanis Has-Yun Amid, Azura Mohd Salleh, Hamzah Yusof, Faridah Mel, Maizirwan A rapid method for large sample number extraction of proteins from mammalian cells |
| title | A rapid method for large sample number extraction of proteins from mammalian cells |
| title_full | A rapid method for large sample number extraction of proteins from mammalian cells |
| title_fullStr | A rapid method for large sample number extraction of proteins from mammalian cells |
| title_full_unstemmed | A rapid method for large sample number extraction of proteins from mammalian cells |
| title_short | A rapid method for large sample number extraction of proteins from mammalian cells |
| title_sort | rapid method for large sample number extraction of proteins from mammalian cells |
| topic | Q Science (General) |
| url | http://irep.iium.edu.my/27821/ http://irep.iium.edu.my/27821/ http://irep.iium.edu.my/27821/1/Paper_-_Packeer-Mohamed_et_al_2012_%28RegRes%29.pdf |