Optimization of cell discruption for recombinant bromelain using high pressure homogenizer (HPH) for commercial production COMMERCIAL PRODUCTION
Recent advancements in recombinant technology have proved to be a promising and effective approach for more sustainable large scale productions of many therapeutic proteins. Nevertheless, since this approach involves expression of protein in a nonnative host microorganism, the overall production...
| Main Authors: | , , , |
|---|---|
| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2012
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/26150/ http://irep.iium.edu.my/26150/1/%5BBPE-106%5D__Muhd_Ezza_Faiez_Othman_Microtribe_2012.pdf |
| _version_ | 1848779595005820928 |
|---|---|
| author | Othman, Muhd. Ezza Faiez Amid, Azura Jimat, Dzun Noraini Jamaluddin, Mohd Jamil Aizat |
| author_facet | Othman, Muhd. Ezza Faiez Amid, Azura Jimat, Dzun Noraini Jamaluddin, Mohd Jamil Aizat |
| author_sort | Othman, Muhd. Ezza Faiez |
| building | IIUM Repository |
| collection | Online Access |
| description | Recent advancements in recombinant technology have proved to be a promising and
effective approach for more sustainable large scale productions of many therapeutic
proteins. Nevertheless, since this approach involves expression of protein in a nonnative
host microorganism, the overall production processes are not quite that straightforward
as several common challenges, such as protein degradation, especially during
cell disruption stage. As the process has been subjected to both protein and hostspecific,
a systematic process “conditioning” for maximal production of recombinant
protein is therefore required. In this study, a simple approach to determine optimal
conditions for cell disruption using high-pressure homogenizer method to isolate
recombinant bromelian from E. coli BL21-AI is reported. One-Factor-At-A-Time
(OFAT) approach was initially used to determine optimal test range for each parameter,
namely, pressure (10-50 MPa), flow rate (10-20 psi) and number of passes (1-3 cycles).
Next, by response surface methodology (RSM) a three factors face-centered central
composite design (FCCD) was applied to obtain optimal process conditions Then the
prediction model was validated. Under the optimal conditions, the specific enzyme
activity of the recombinant bromelain was found to be functionally and reproducibly
acceptable at 0.037 U/mg. |
| first_indexed | 2025-11-14T15:20:16Z |
| format | Proceeding Paper |
| id | iium-26150 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:20:16Z |
| publishDate | 2012 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-261502013-01-04T03:00:21Z http://irep.iium.edu.my/26150/ Optimization of cell discruption for recombinant bromelain using high pressure homogenizer (HPH) for commercial production COMMERCIAL PRODUCTION Othman, Muhd. Ezza Faiez Amid, Azura Jimat, Dzun Noraini Jamaluddin, Mohd Jamil Aizat T175 Industrial research. Research and development Recent advancements in recombinant technology have proved to be a promising and effective approach for more sustainable large scale productions of many therapeutic proteins. Nevertheless, since this approach involves expression of protein in a nonnative host microorganism, the overall production processes are not quite that straightforward as several common challenges, such as protein degradation, especially during cell disruption stage. As the process has been subjected to both protein and hostspecific, a systematic process “conditioning” for maximal production of recombinant protein is therefore required. In this study, a simple approach to determine optimal conditions for cell disruption using high-pressure homogenizer method to isolate recombinant bromelian from E. coli BL21-AI is reported. One-Factor-At-A-Time (OFAT) approach was initially used to determine optimal test range for each parameter, namely, pressure (10-50 MPa), flow rate (10-20 psi) and number of passes (1-3 cycles). Next, by response surface methodology (RSM) a three factors face-centered central composite design (FCCD) was applied to obtain optimal process conditions Then the prediction model was validated. Under the optimal conditions, the specific enzyme activity of the recombinant bromelain was found to be functionally and reproducibly acceptable at 0.037 U/mg. 2012 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/26150/1/%5BBPE-106%5D__Muhd_Ezza_Faiez_Othman_Microtribe_2012.pdf Othman, Muhd. Ezza Faiez and Amid, Azura and Jimat, Dzun Noraini and Jamaluddin, Mohd Jamil Aizat (2012) Optimization of cell discruption for recombinant bromelain using high pressure homogenizer (HPH) for commercial production COMMERCIAL PRODUCTION. In: Malaysian International Conference on Trends in Bioprocess Engineering (MICOTriBE 2009), 12-13 December 2012, Langkawi, Kedah, Malaysia. (Unpublished) |
| spellingShingle | T175 Industrial research. Research and development Othman, Muhd. Ezza Faiez Amid, Azura Jimat, Dzun Noraini Jamaluddin, Mohd Jamil Aizat Optimization of cell discruption for recombinant bromelain using high pressure homogenizer (HPH) for commercial production COMMERCIAL PRODUCTION |
| title | Optimization of cell discruption for recombinant bromelain using high pressure homogenizer (HPH) for commercial production
COMMERCIAL PRODUCTION |
| title_full | Optimization of cell discruption for recombinant bromelain using high pressure homogenizer (HPH) for commercial production
COMMERCIAL PRODUCTION |
| title_fullStr | Optimization of cell discruption for recombinant bromelain using high pressure homogenizer (HPH) for commercial production
COMMERCIAL PRODUCTION |
| title_full_unstemmed | Optimization of cell discruption for recombinant bromelain using high pressure homogenizer (HPH) for commercial production
COMMERCIAL PRODUCTION |
| title_short | Optimization of cell discruption for recombinant bromelain using high pressure homogenizer (HPH) for commercial production
COMMERCIAL PRODUCTION |
| title_sort | optimization of cell discruption for recombinant bromelain using high pressure homogenizer (hph) for commercial production
commercial production |
| topic | T175 Industrial research. Research and development |
| url | http://irep.iium.edu.my/26150/ http://irep.iium.edu.my/26150/1/%5BBPE-106%5D__Muhd_Ezza_Faiez_Othman_Microtribe_2012.pdf |