Immbolization of lipase on multi-walled carbon nanotubes
Immobilized enzymes, such as lipases, have diverse applications including chemical, fuel, pharmaceutical and food processing industries. In this study, commercially available lipase extracted from Aspergillus niger has been immobilized on (MWNTs). The enzyme was bound to carbon nanotubes by covalent...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English |
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2008
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| Online Access: | http://irep.iium.edu.my/2613/ http://irep.iium.edu.my/2613/1/009_ALKHATIB_M.F._Malaysia.pdf |
| _version_ | 1848776027049820160 |
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| author | Alkhatib, Ma'an Salleh, Hamzah Sultan, Anans M. |
| author_facet | Alkhatib, Ma'an Salleh, Hamzah Sultan, Anans M. |
| author_sort | Alkhatib, Ma'an |
| building | IIUM Repository |
| collection | Online Access |
| description | Immobilized enzymes, such as lipases, have diverse applications including chemical, fuel, pharmaceutical and food processing industries. In this study, commercially available lipase extracted from Aspergillus niger has been immobilized on (MWNTs). The enzyme was bound to carbon nanotubes by covalent bonding, in the presence of N-(3-dimethylaminopropyl)-N'-ethyl)-carbodiimide (EDC) as a coupling reagent. Carbon nanotubes were carboxylated by sonication in 1 M nitric acid prior to treatment with the coupling reagent. Native lipase showed an activity of 2.22 x 10-3 U, whereas, immobilized lipase demonstrated an activity of 0.335 x 10-3, retaining 55 % of the native activity given that only 27.4 % enzyme immobilization has been achieved. One unit is defined as 1nmol of p-nitrophenol released by the hydrolysis of the substrate 4-nitrophenyl palmitate (pNPP) per 1 ml per minute. |
| first_indexed | 2025-11-14T14:23:33Z |
| format | Proceeding Paper |
| id | iium-2613 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:23:33Z |
| publishDate | 2008 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-26132011-11-16T14:20:44Z http://irep.iium.edu.my/2613/ Immbolization of lipase on multi-walled carbon nanotubes Alkhatib, Ma'an Salleh, Hamzah Sultan, Anans M. TA401 Materials of engineering and construction TP248.13 Biotechnology Immobilized enzymes, such as lipases, have diverse applications including chemical, fuel, pharmaceutical and food processing industries. In this study, commercially available lipase extracted from Aspergillus niger has been immobilized on (MWNTs). The enzyme was bound to carbon nanotubes by covalent bonding, in the presence of N-(3-dimethylaminopropyl)-N'-ethyl)-carbodiimide (EDC) as a coupling reagent. Carbon nanotubes were carboxylated by sonication in 1 M nitric acid prior to treatment with the coupling reagent. Native lipase showed an activity of 2.22 x 10-3 U, whereas, immobilized lipase demonstrated an activity of 0.335 x 10-3, retaining 55 % of the native activity given that only 27.4 % enzyme immobilization has been achieved. One unit is defined as 1nmol of p-nitrophenol released by the hydrolysis of the substrate 4-nitrophenyl palmitate (pNPP) per 1 ml per minute. 2008 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/2613/1/009_ALKHATIB_M.F._Malaysia.pdf Alkhatib, Ma'an and Salleh, Hamzah and Sultan, Anans M. (2008) Immbolization of lipase on multi-walled carbon nanotubes. In: 2008 International Conference on Environment (ICENV 2008), 15-17 December, 2008, G Hotel, Penang. (Unpublished) |
| spellingShingle | TA401 Materials of engineering and construction TP248.13 Biotechnology Alkhatib, Ma'an Salleh, Hamzah Sultan, Anans M. Immbolization of lipase on multi-walled carbon nanotubes |
| title | Immbolization of lipase on multi-walled carbon nanotubes |
| title_full | Immbolization of lipase on multi-walled carbon nanotubes |
| title_fullStr | Immbolization of lipase on multi-walled carbon nanotubes |
| title_full_unstemmed | Immbolization of lipase on multi-walled carbon nanotubes |
| title_short | Immbolization of lipase on multi-walled carbon nanotubes |
| title_sort | immbolization of lipase on multi-walled carbon nanotubes |
| topic | TA401 Materials of engineering and construction TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/2613/ http://irep.iium.edu.my/2613/1/009_ALKHATIB_M.F._Malaysia.pdf |