Cycloartane triterpenoid as an insulin mimicking agent
The incidence of type 2 diabetes and the resultant metabolic syndrome are rapidly increasing. In this study, we investigated the effects of cycloartane triterpenoids on the adipocyte differentiation and glucose uptake in 3T3-L1 adipocytes together with its mechanism. The cells were treated for lipid...
| Main Authors: | , , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2012
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| Online Access: | http://irep.iium.edu.my/25744/ http://irep.iium.edu.my/25744/1/Deny_Susanti.pdf |
| _version_ | 1848779511954407424 |
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| author | Darnis, Deny Susanti Mohamed Amiroudine, Mohamed Zaffar Ali Bakhtiar, M. Taher Ichwan, Solachuddin J. A. Kaderi, Mohd Arifin |
| author_facet | Darnis, Deny Susanti Mohamed Amiroudine, Mohamed Zaffar Ali Bakhtiar, M. Taher Ichwan, Solachuddin J. A. Kaderi, Mohd Arifin |
| author_sort | Darnis, Deny Susanti |
| building | IIUM Repository |
| collection | Online Access |
| description | The incidence of type 2 diabetes and the resultant metabolic syndrome are rapidly increasing. In this study, we investigated the effects of cycloartane triterpenoids on the adipocyte differentiation and glucose uptake in 3T3-L1 adipocytes together with its mechanism. The cells were treated for lipid accumulation with different concentration of cycloartane by using Oil Red O staining. After day-8 of treatment, morphological changes and high lipid accumulation effects were observed in these cells (p<0.05). In addition, the intracellular fat accumulation increased by 1.9 fold relative to MDI-treated control cells at concentration of 50 µM. The insulin-induced 2-deoxy-D-[3H] glucose uptake also was significantly increased (p<0.01) in cycloartane treated cells as compared to the basal. Moreover, the adipocytes differentiated by cycloartane exhibited insulin sensitivity as demonstrated by a significant increase in glucose uptake. Further evaluation of the mechanism of related gene expression by using the quantitative RT-PCR shows that mature adipocytes treated with cycloartane increased the expression levels of PPARγ which regulate the adipogenesis process and its target gene GLUT4 that involve in glucose uptake. In conclusion, these findings suggest that cycloartane triterpenoids from adipocyte differentiation and glucose uptake by stimulation of PPARγ and GLUT4 expression. |
| first_indexed | 2025-11-14T15:18:57Z |
| format | Proceeding Paper |
| id | iium-25744 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:18:57Z |
| publishDate | 2012 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-257442022-06-08T06:40:55Z http://irep.iium.edu.my/25744/ Cycloartane triterpenoid as an insulin mimicking agent Darnis, Deny Susanti Mohamed Amiroudine, Mohamed Zaffar Ali Bakhtiar, M. Taher Ichwan, Solachuddin J. A. Kaderi, Mohd Arifin RS Pharmacy and materia medica The incidence of type 2 diabetes and the resultant metabolic syndrome are rapidly increasing. In this study, we investigated the effects of cycloartane triterpenoids on the adipocyte differentiation and glucose uptake in 3T3-L1 adipocytes together with its mechanism. The cells were treated for lipid accumulation with different concentration of cycloartane by using Oil Red O staining. After day-8 of treatment, morphological changes and high lipid accumulation effects were observed in these cells (p<0.05). In addition, the intracellular fat accumulation increased by 1.9 fold relative to MDI-treated control cells at concentration of 50 µM. The insulin-induced 2-deoxy-D-[3H] glucose uptake also was significantly increased (p<0.01) in cycloartane treated cells as compared to the basal. Moreover, the adipocytes differentiated by cycloartane exhibited insulin sensitivity as demonstrated by a significant increase in glucose uptake. Further evaluation of the mechanism of related gene expression by using the quantitative RT-PCR shows that mature adipocytes treated with cycloartane increased the expression levels of PPARγ which regulate the adipogenesis process and its target gene GLUT4 that involve in glucose uptake. In conclusion, these findings suggest that cycloartane triterpenoids from adipocyte differentiation and glucose uptake by stimulation of PPARγ and GLUT4 expression. 2012 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/25744/1/Deny_Susanti.pdf Darnis, Deny Susanti and Mohamed Amiroudine, Mohamed Zaffar Ali and Bakhtiar, M. Taher and Ichwan, Solachuddin J. A. and Kaderi, Mohd Arifin (2012) Cycloartane triterpenoid as an insulin mimicking agent. In: The 24th FAPA Congress 2012, 13-16 September 2012, Bali. http://www.fapa2012.com |
| spellingShingle | RS Pharmacy and materia medica Darnis, Deny Susanti Mohamed Amiroudine, Mohamed Zaffar Ali Bakhtiar, M. Taher Ichwan, Solachuddin J. A. Kaderi, Mohd Arifin Cycloartane triterpenoid as an insulin mimicking agent |
| title | Cycloartane triterpenoid as an insulin mimicking agent |
| title_full | Cycloartane triterpenoid as an insulin mimicking agent |
| title_fullStr | Cycloartane triterpenoid as an insulin mimicking agent |
| title_full_unstemmed | Cycloartane triterpenoid as an insulin mimicking agent |
| title_short | Cycloartane triterpenoid as an insulin mimicking agent |
| title_sort | cycloartane triterpenoid as an insulin mimicking agent |
| topic | RS Pharmacy and materia medica |
| url | http://irep.iium.edu.my/25744/ http://irep.iium.edu.my/25744/ http://irep.iium.edu.my/25744/1/Deny_Susanti.pdf |