Enzymatic hydrolysis of granular native and mildly heat-treated tapioca and sweet potato starches at sub-gelatinization temperature

The effect of mild heat treatment (below gelatinization temperature) towards the susceptibility of granular starch to enzymatic hydrolysis was investigated. Tapioca and sweet potato starches were subjected to enzymatic hydrolysis with a mixture of fungal a-amylase and glucoamylase at 35 1C for 24...

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Main Authors: Y. N., Shariffa, Al-Jashamy, Karim A., A., Fazilah, Sarker, Md. Zaidul Islam
Format: Article
Language:English
Published: Elsevier Inc. 2009
Subjects:
Online Access:http://irep.iium.edu.my/24836/
http://irep.iium.edu.my/24836/1/Food_Hydro-Colloids-2.pdf
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author Y. N., Shariffa
Al-Jashamy, Karim A.
A., Fazilah
Sarker, Md. Zaidul Islam
author_facet Y. N., Shariffa
Al-Jashamy, Karim A.
A., Fazilah
Sarker, Md. Zaidul Islam
author_sort Y. N., Shariffa
building IIUM Repository
collection Online Access
description The effect of mild heat treatment (below gelatinization temperature) towards the susceptibility of granular starch to enzymatic hydrolysis was investigated. Tapioca and sweet potato starches were subjected to enzymatic hydrolysis with a mixture of fungal a-amylase and glucoamylase at 35 1C for 24 h. Starches were hydrolyzed in native (granular) state and after heat treatment below gelatinization temperature (60 1C for 30min). The dextrose equivalent (DE) value of heat-treated starch increased significantly compared to native starch, i.e., 36–50% and 27–34% for tapioca and sweet potato starch, respectively. Scanning electron microscopy examination showed that enzymatic erosion occurred mainly at the surface of starch granules. Hydrolyzed heat-treated starch exhibited rougher surface and porous granules compared to native starch. X-ray analysis suggested that enzymatic erosion preferentially occurred in amorphous areas of the granules. The amylose content, swelling power and solubility showed insignificant increase for both starches. Evidently, heating treatment below gelatinization temperature was effective in enhancing the degree of hydrolysis of granular starch.
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institution International Islamic University Malaysia
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language English
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publishDate 2009
publisher Elsevier Inc.
recordtype eprints
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spelling iium-248362013-01-18T06:17:25Z http://irep.iium.edu.my/24836/ Enzymatic hydrolysis of granular native and mildly heat-treated tapioca and sweet potato starches at sub-gelatinization temperature Y. N., Shariffa Al-Jashamy, Karim A. A., Fazilah Sarker, Md. Zaidul Islam QD Chemistry The effect of mild heat treatment (below gelatinization temperature) towards the susceptibility of granular starch to enzymatic hydrolysis was investigated. Tapioca and sweet potato starches were subjected to enzymatic hydrolysis with a mixture of fungal a-amylase and glucoamylase at 35 1C for 24 h. Starches were hydrolyzed in native (granular) state and after heat treatment below gelatinization temperature (60 1C for 30min). The dextrose equivalent (DE) value of heat-treated starch increased significantly compared to native starch, i.e., 36–50% and 27–34% for tapioca and sweet potato starch, respectively. Scanning electron microscopy examination showed that enzymatic erosion occurred mainly at the surface of starch granules. Hydrolyzed heat-treated starch exhibited rougher surface and porous granules compared to native starch. X-ray analysis suggested that enzymatic erosion preferentially occurred in amorphous areas of the granules. The amylose content, swelling power and solubility showed insignificant increase for both starches. Evidently, heating treatment below gelatinization temperature was effective in enhancing the degree of hydrolysis of granular starch. Elsevier Inc. 2009 Article PeerReviewed application/pdf en cc_by_nd http://irep.iium.edu.my/24836/1/Food_Hydro-Colloids-2.pdf Y. N., Shariffa and Al-Jashamy, Karim A. and A., Fazilah and Sarker, Md. Zaidul Islam (2009) Enzymatic hydrolysis of granular native and mildly heat-treated tapioca and sweet potato starches at sub-gelatinization temperature. Food Hydrocolloids, 23 (2). pp. 434-440. ISSN 0268005X http://dx.doi.org/10.1016/j.foodhyd.2008.03.009 doi:10.1016/j.foodhyd.2008.03.009
spellingShingle QD Chemistry
Y. N., Shariffa
Al-Jashamy, Karim A.
A., Fazilah
Sarker, Md. Zaidul Islam
Enzymatic hydrolysis of granular native and mildly heat-treated tapioca and sweet potato starches at sub-gelatinization temperature
title Enzymatic hydrolysis of granular native and mildly heat-treated tapioca and sweet potato starches at sub-gelatinization temperature
title_full Enzymatic hydrolysis of granular native and mildly heat-treated tapioca and sweet potato starches at sub-gelatinization temperature
title_fullStr Enzymatic hydrolysis of granular native and mildly heat-treated tapioca and sweet potato starches at sub-gelatinization temperature
title_full_unstemmed Enzymatic hydrolysis of granular native and mildly heat-treated tapioca and sweet potato starches at sub-gelatinization temperature
title_short Enzymatic hydrolysis of granular native and mildly heat-treated tapioca and sweet potato starches at sub-gelatinization temperature
title_sort enzymatic hydrolysis of granular native and mildly heat-treated tapioca and sweet potato starches at sub-gelatinization temperature
topic QD Chemistry
url http://irep.iium.edu.my/24836/
http://irep.iium.edu.my/24836/
http://irep.iium.edu.my/24836/
http://irep.iium.edu.my/24836/1/Food_Hydro-Colloids-2.pdf