Kinetic study of a Bacterial Consortium isolated from soil contaminated with crude oil
Laboratory study was carried out to isolate hydrocarbon utilizing bacteria from crude-oil contaminated soil (COCS) to test their potential for the bioremediation of COCS. Twenty strains were isolated by enrichment culture using minimal salt medium with crude oil as sole carbon source. The twenty iso...
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| Format: | Article |
| Language: | English |
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AJBAS Journal
2011
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| Online Access: | http://irep.iium.edu.my/2478/ http://irep.iium.edu.my/2478/1/AJBAS_2011_925-930.pdf |
| _version_ | 1848776010176135168 |
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| author | Husain, Iman A. F. Al-Khatib, Ma'an Fahmi Rashid Alam, Md. Zahangir Muyibi, Suleyman Aremu |
| author_facet | Husain, Iman A. F. Al-Khatib, Ma'an Fahmi Rashid Alam, Md. Zahangir Muyibi, Suleyman Aremu |
| author_sort | Husain, Iman A. F. |
| building | IIUM Repository |
| collection | Online Access |
| description | Laboratory study was carried out to isolate hydrocarbon utilizing bacteria from crude-oil contaminated soil (COCS) to test their potential for the bioremediation of COCS. Twenty strains were isolated by enrichment culture using minimal salt medium with crude oil as sole carbon source. The twenty isolated strains were purified and monitored for their growth by monitoring the optical density (OD600). The twelve best growing strains were selected to form four bacterial consortiums. Each consortium contains three strains combined with accordance to their growth. The four consortiums were tested for their growth and the best one (Cons.1) was selected for the kinetics study and the biodegradability test. From the first order kinetic study, the maximum growth rate μmax of the selected consortium was 0.02 hr-1 while the half saturation constant ks was 2.2%v/v total petroleum hydrocarbons (TPH). The selected strains showed a potential for biodegradation of crude oil in soil
slurry with removal up to 95% within seven days which would further be used for the bioremediation of COCS. |
| first_indexed | 2025-11-14T14:23:17Z |
| format | Article |
| id | iium-2478 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:23:17Z |
| publishDate | 2011 |
| publisher | AJBAS Journal |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-24782024-05-02T08:28:02Z http://irep.iium.edu.my/2478/ Kinetic study of a Bacterial Consortium isolated from soil contaminated with crude oil Husain, Iman A. F. Al-Khatib, Ma'an Fahmi Rashid Alam, Md. Zahangir Muyibi, Suleyman Aremu TD172 Environmental pollution TP248.13 Biotechnology Laboratory study was carried out to isolate hydrocarbon utilizing bacteria from crude-oil contaminated soil (COCS) to test their potential for the bioremediation of COCS. Twenty strains were isolated by enrichment culture using minimal salt medium with crude oil as sole carbon source. The twenty isolated strains were purified and monitored for their growth by monitoring the optical density (OD600). The twelve best growing strains were selected to form four bacterial consortiums. Each consortium contains three strains combined with accordance to their growth. The four consortiums were tested for their growth and the best one (Cons.1) was selected for the kinetics study and the biodegradability test. From the first order kinetic study, the maximum growth rate μmax of the selected consortium was 0.02 hr-1 while the half saturation constant ks was 2.2%v/v total petroleum hydrocarbons (TPH). The selected strains showed a potential for biodegradation of crude oil in soil slurry with removal up to 95% within seven days which would further be used for the bioremediation of COCS. AJBAS Journal 2011 Article PeerReviewed application/pdf en http://irep.iium.edu.my/2478/1/AJBAS_2011_925-930.pdf Husain, Iman A. F. and Al-Khatib, Ma'an Fahmi Rashid and Alam, Md. Zahangir and Muyibi, Suleyman Aremu (2011) Kinetic study of a Bacterial Consortium isolated from soil contaminated with crude oil. Australian Journal of Basic and Applied Sciences, 5 (6). pp. 925-930. ISSN 1991-8178 http://www.insipub.com/ajbas.html |
| spellingShingle | TD172 Environmental pollution TP248.13 Biotechnology Husain, Iman A. F. Al-Khatib, Ma'an Fahmi Rashid Alam, Md. Zahangir Muyibi, Suleyman Aremu Kinetic study of a Bacterial Consortium isolated from soil contaminated with crude oil |
| title | Kinetic study of a Bacterial Consortium isolated from soil contaminated with crude oil |
| title_full | Kinetic study of a Bacterial Consortium isolated from soil contaminated with crude oil |
| title_fullStr | Kinetic study of a Bacterial Consortium isolated from soil contaminated with crude oil |
| title_full_unstemmed | Kinetic study of a Bacterial Consortium isolated from soil contaminated with crude oil |
| title_short | Kinetic study of a Bacterial Consortium isolated from soil contaminated with crude oil |
| title_sort | kinetic study of a bacterial consortium isolated from soil contaminated with crude oil |
| topic | TD172 Environmental pollution TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/2478/ http://irep.iium.edu.my/2478/ http://irep.iium.edu.my/2478/1/AJBAS_2011_925-930.pdf |