Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma
Introduction: Synovial Sarcoma is a rare cancer and account for 5-10% of adult soft tissue sarcomas. The tumour exhibits unspecified histogenesis composed primarily of spindle cells with variable epithelial components. Despite establishment of some immunohistochemistry staining, making a definitive...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English |
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2012
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| Online Access: | http://irep.iium.edu.my/24612/ http://irep.iium.edu.my/24612/1/Abstract_CPath2012.pdf |
| _version_ | 1848779330567536640 |
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| author | Effendy Goon, Danial Harun, Norra Muhammad, Siti Aeshah @ Naznin A.Talib, Norlelawati |
| author_facet | Effendy Goon, Danial Harun, Norra Muhammad, Siti Aeshah @ Naznin A.Talib, Norlelawati |
| author_sort | Effendy Goon, Danial |
| building | IIUM Repository |
| collection | Online Access |
| description | Introduction: Synovial Sarcoma is a rare cancer and account for 5-10% of adult soft tissue sarcomas. The tumour exhibits unspecified histogenesis composed primarily of spindle cells with variable epithelial components. Despite establishment of some immunohistochemistry staining, making a definitive diagnosis of synovial sarcoma remains a challenging task. This is due to the histo-morphology and immunophenotypes similarities of this tumour to other types of soft tissue sarcoma. Objective: The current study aims to apply a molecular method for detection of SYT-SSX fusion transcript, a common molecular defect (>90% of the cases) in Synovial Sarcoma irrespective of the histologic subtypes. Method: Paraffin-embedded fixed-tissue (PEFT) blocks of 3 confirmed and 15 possible cases of Synovial Sarcoma were retrieved from Department of Pathology, Tengku Ampuan-Afzan Hospital, Kuantan and subjected to RNA purification using the standard spin column protocol. A one step direct RT-PCR was performed using SYT-SSX and PBGD primer sets for detection of SYT-SSX fusion gene and the reference gene PBGD respectively. Results: Our preliminary molecular analyses showed positive SYT-SSX fusion transcript in all 3 confirmed cases and 5 possible cases of synovial sarcoma. Further analysis is still on going for the remaining samples. Conclusion: Molecular detection of SYT-SSX fusion-transcript is useful in establishing the diagnosis of Synovial Sarcoma. |
| first_indexed | 2025-11-14T15:16:04Z |
| format | Proceeding Paper |
| id | iium-24612 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:16:04Z |
| publishDate | 2012 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-246122022-04-07T02:09:27Z http://irep.iium.edu.my/24612/ Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma Effendy Goon, Danial Harun, Norra Muhammad, Siti Aeshah @ Naznin A.Talib, Norlelawati RB Pathology Introduction: Synovial Sarcoma is a rare cancer and account for 5-10% of adult soft tissue sarcomas. The tumour exhibits unspecified histogenesis composed primarily of spindle cells with variable epithelial components. Despite establishment of some immunohistochemistry staining, making a definitive diagnosis of synovial sarcoma remains a challenging task. This is due to the histo-morphology and immunophenotypes similarities of this tumour to other types of soft tissue sarcoma. Objective: The current study aims to apply a molecular method for detection of SYT-SSX fusion transcript, a common molecular defect (>90% of the cases) in Synovial Sarcoma irrespective of the histologic subtypes. Method: Paraffin-embedded fixed-tissue (PEFT) blocks of 3 confirmed and 15 possible cases of Synovial Sarcoma were retrieved from Department of Pathology, Tengku Ampuan-Afzan Hospital, Kuantan and subjected to RNA purification using the standard spin column protocol. A one step direct RT-PCR was performed using SYT-SSX and PBGD primer sets for detection of SYT-SSX fusion gene and the reference gene PBGD respectively. Results: Our preliminary molecular analyses showed positive SYT-SSX fusion transcript in all 3 confirmed cases and 5 possible cases of synovial sarcoma. Further analysis is still on going for the remaining samples. Conclusion: Molecular detection of SYT-SSX fusion-transcript is useful in establishing the diagnosis of Synovial Sarcoma. 2012 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/24612/1/Abstract_CPath2012.pdf Effendy Goon, Danial and Harun, Norra and Muhammad, Siti Aeshah @ Naznin and A.Talib, Norlelawati (2012) Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma. In: 11th Annual Scientific Meeting, College of Pathologist Malaysia, 8-10 June 2012, Crowne Plaza Mutiara, Kuala Lumpur. |
| spellingShingle | RB Pathology Effendy Goon, Danial Harun, Norra Muhammad, Siti Aeshah @ Naznin A.Talib, Norlelawati Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma |
| title | Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma |
| title_full | Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma |
| title_fullStr | Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma |
| title_full_unstemmed | Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma |
| title_short | Preliminary analyses on detection of SYT-SSX fusion-transcripts in Synovial Sarcoma |
| title_sort | preliminary analyses on detection of syt-ssx fusion-transcripts in synovial sarcoma |
| topic | RB Pathology |
| url | http://irep.iium.edu.my/24612/ http://irep.iium.edu.my/24612/1/Abstract_CPath2012.pdf |