Modeling the growth kinetics of Escherichia Coli fermentation in bioreactors
ABSTRACT The chapter focuses on the growth kinetics and modelling of recombinant Escherichia coli in fermentation processes. Experimental data for from four bioreactors were analyzed using Monod, Logistic and Contois models. A comparison between the models' predicted values of cell, substrat...
| Main Authors: | , , |
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| Format: | Book Chapter |
| Language: | English |
| Published: |
IIUM Press
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/24222/ http://irep.iium.edu.my/24222/3/Modeling_the_Growth.pdf |
| _version_ | 1848779267801874432 |
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| author | Ibrahim Ali , Noorbatcha Wan Norulhazrina, Wan Hassan Maizirwan , Mel |
| author_facet | Ibrahim Ali , Noorbatcha Wan Norulhazrina, Wan Hassan Maizirwan , Mel |
| author_sort | Ibrahim Ali , Noorbatcha |
| building | IIUM Repository |
| collection | Online Access |
| description | ABSTRACT
The chapter focuses on the growth kinetics and modelling of recombinant Escherichia coli in fermentation processes. Experimental data for from four bioreactors were analyzed using Monod, Logistic and Contois models. A comparison between the models' predicted values of cell, substrate and product (β-glucuronidase enzyme) was done with the kinetic parameters mmax, YX/S, Ks, k1, and k2 calculated from the experimental data. Substrate used for this modelling was glucose while the independent variables were peptone soy, sodium chloride and yeast extract. The Logistic model is found to describe the growth of E. coli in the fermentation process with the limited supply of glucose and the Monod and Contois models failed to represent the cell growth profiles.
Keywords: growth kinetics, modeling, recombinant E. coli, fermentation.
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| first_indexed | 2025-11-14T15:15:04Z |
| format | Book Chapter |
| id | iium-24222 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:15:04Z |
| publishDate | 2011 |
| publisher | IIUM Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-242222012-08-07T06:21:56Z http://irep.iium.edu.my/24222/ Modeling the growth kinetics of Escherichia Coli fermentation in bioreactors Ibrahim Ali , Noorbatcha Wan Norulhazrina, Wan Hassan Maizirwan , Mel TP248.13 Biotechnology ABSTRACT The chapter focuses on the growth kinetics and modelling of recombinant Escherichia coli in fermentation processes. Experimental data for from four bioreactors were analyzed using Monod, Logistic and Contois models. A comparison between the models' predicted values of cell, substrate and product (β-glucuronidase enzyme) was done with the kinetic parameters mmax, YX/S, Ks, k1, and k2 calculated from the experimental data. Substrate used for this modelling was glucose while the independent variables were peptone soy, sodium chloride and yeast extract. The Logistic model is found to describe the growth of E. coli in the fermentation process with the limited supply of glucose and the Monod and Contois models failed to represent the cell growth profiles. Keywords: growth kinetics, modeling, recombinant E. coli, fermentation. IIUM Press 2011 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/24222/3/Modeling_the_Growth.pdf Ibrahim Ali , Noorbatcha and Wan Norulhazrina, Wan Hassan and Maizirwan , Mel (2011) Modeling the growth kinetics of Escherichia Coli fermentation in bioreactors. In: Bioprocessing of Recombinant E. Coli producing beta-glucuronidase enzyme. IIUM Press, Kuala Lumpur, Malaysia, pp. 115-123. ISBN 9789674180102 http://rms.research.iium.edu.my/bookstore/default.aspx |
| spellingShingle | TP248.13 Biotechnology Ibrahim Ali , Noorbatcha Wan Norulhazrina, Wan Hassan Maizirwan , Mel Modeling the growth kinetics of Escherichia Coli fermentation in bioreactors |
| title | Modeling the growth kinetics of Escherichia Coli fermentation in bioreactors |
| title_full | Modeling the growth kinetics of Escherichia Coli fermentation in bioreactors |
| title_fullStr | Modeling the growth kinetics of Escherichia Coli fermentation in bioreactors |
| title_full_unstemmed | Modeling the growth kinetics of Escherichia Coli fermentation in bioreactors |
| title_short | Modeling the growth kinetics of Escherichia Coli fermentation in bioreactors |
| title_sort | modeling the growth kinetics of escherichia coli fermentation in bioreactors |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/24222/ http://irep.iium.edu.my/24222/ http://irep.iium.edu.my/24222/3/Modeling_the_Growth.pdf |