Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh
Characterization and quantification of carotenoid compound is complicated, costly and timeconsuming. The accuracy and reliability of the data depend solely on the standard and to High Performance Liquid Chromatography (HPLC) analysis but the major constraint is to acquire and to maintain the pure...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Faculty of Food Science and Technology, Universiti Putra Malaysia
2012
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/24207/ http://irep.iium.edu.my/24207/1/Scheme_of_obtaining_%CE%B2-carotene_standard.pdf |
| _version_ | 1848779264607911936 |
|---|---|
| author | Shahidan, Norshazila Jaswir, Irwandi Othman, Rashidi Hashim, Yumi Zuhanis Has-Yun |
| author_facet | Shahidan, Norshazila Jaswir, Irwandi Othman, Rashidi Hashim, Yumi Zuhanis Has-Yun |
| author_sort | Shahidan, Norshazila |
| building | IIUM Repository |
| collection | Online Access |
| description | Characterization and quantification of carotenoid compound is complicated, costly and timeconsuming.
The accuracy and reliability of the data depend solely on the standard and to High Performance
Liquid Chromatography (HPLC) analysis but the major constraint is to acquire and to maintain the pure standards.
Carotenoid standards are commercially available but they are expensive and are prone to isomerization and
oxidation. Thus, the purpose of this study is to establish an analytical method for isolating β-carotene by using
open column chromatography (OCC) from pumpkin (Cucurbita moschata) to be used as one of the carotenoid
standards for determination of total and individual carotenoid. Pumpkin with orange flesh has been chosen
due to the non-seasonal nature and its availability all year-round. This study demonstrated that the purity of
β-carotene standard; determined by HPLC was ranged from 92.21 to 97.95%. The standard curves with five
different concentrations of β-carotene extract from pumpkins in triplicate were constructed by plotting the peak
area against the concentration. The coefficient of correlation was 0.9936. Therefore, this study established that
pumpkin can be a reliable source of beta-carotene standard as it is cheap and commonly available throughout
the year. |
| first_indexed | 2025-11-14T15:15:01Z |
| format | Article |
| id | iium-24207 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:15:01Z |
| publishDate | 2012 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-242072021-07-23T16:18:46Z http://irep.iium.edu.my/24207/ Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh Shahidan, Norshazila Jaswir, Irwandi Othman, Rashidi Hashim, Yumi Zuhanis Has-Yun Q Science (General) Characterization and quantification of carotenoid compound is complicated, costly and timeconsuming. The accuracy and reliability of the data depend solely on the standard and to High Performance Liquid Chromatography (HPLC) analysis but the major constraint is to acquire and to maintain the pure standards. Carotenoid standards are commercially available but they are expensive and are prone to isomerization and oxidation. Thus, the purpose of this study is to establish an analytical method for isolating β-carotene by using open column chromatography (OCC) from pumpkin (Cucurbita moschata) to be used as one of the carotenoid standards for determination of total and individual carotenoid. Pumpkin with orange flesh has been chosen due to the non-seasonal nature and its availability all year-round. This study demonstrated that the purity of β-carotene standard; determined by HPLC was ranged from 92.21 to 97.95%. The standard curves with five different concentrations of β-carotene extract from pumpkins in triplicate were constructed by plotting the peak area against the concentration. The coefficient of correlation was 0.9936. Therefore, this study established that pumpkin can be a reliable source of beta-carotene standard as it is cheap and commonly available throughout the year. Faculty of Food Science and Technology, Universiti Putra Malaysia 2012 Article PeerReviewed application/pdf en http://irep.iium.edu.my/24207/1/Scheme_of_obtaining_%CE%B2-carotene_standard.pdf Shahidan, Norshazila and Jaswir, Irwandi and Othman, Rashidi and Hashim, Yumi Zuhanis Has-Yun (2012) Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh. International Food Research Journal, 19 (2). pp. 531-535. ISSN 19854668 (P), 22317546 (O) http://www.ifrj.upm.edu.my/19%20(02)%202012/(23)IFRJ-2012%20Irwandi.pdf |
| spellingShingle | Q Science (General) Shahidan, Norshazila Jaswir, Irwandi Othman, Rashidi Hashim, Yumi Zuhanis Has-Yun Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh |
| title | Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh |
| title_full | Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh |
| title_fullStr | Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh |
| title_full_unstemmed | Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh |
| title_short | Scheme of obtaining ß-carotene standard from pumpkin (Cucurbita moschata) flesh |
| title_sort | scheme of obtaining ß-carotene standard from pumpkin (cucurbita moschata) flesh |
| topic | Q Science (General) |
| url | http://irep.iium.edu.my/24207/ http://irep.iium.edu.my/24207/ http://irep.iium.edu.my/24207/1/Scheme_of_obtaining_%CE%B2-carotene_standard.pdf |