Expression, purification, and characterization of a recombinant stem bromelain from ananas comosus

QR Code

Expression, purification, and characterization of a recombinant stem bromelain from ananas comosus

Commercially available bromelain is prepared by performing a tedious and costly purification method that provides multiple degrees of purified bromelain. In the current study, a gene encoding stem bromelain from Ananas comosus was amplified using polymerase chain reaction (PCR). This bromelain gene...

Full description

Bibliographic Details
Main Authors:	Amid, Azura, Ismail, Nurul Azira, Yusof, Faridah, Mohd. Salleh, Hamzah
Format:	Article
Language:	English
Published:	ELSEVIER 2011
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/2374/ http://irep.iium.edu.my/2374/1/Bromelain_cloning.pdf

Similar Items

Molecular cloning sequencing purification of stem bromelain from Ananas comosus
by: Amid, Azura, et al.
Published: (2010)

Comparative structural analysis of fruit and stem bromelain from Ananas comosus
by: Aizi Nor Mazila, Ramli, et al.
Published: (2018)

Molecular and bioinformatics characterization of fruit bromelain from ananas comosus
by: Tuan Norsyalieza, Tuan Aznan
Published: (2018)

Molecular cloning and overexpression of stem bromelain from Ananas comosus in Kluyveromyces lactis
by: Ramli, Aizi Nor Mazila
Published: (2017)

Observation on recombinant bromelain activity before and after purification
by: Ismail, Nurul Azira, et al.
Published: (2010)

Enzymatic analysis and characterization of bromelain from two varieties of pineapple (Ananas comosus) fruit and stem extracts
by: Norliana, Munir, et al.
Published: (2022)

Comparative structural analysis of fruit and stem bromelain from Ananas comosus
by: Ramli, Aizi Nor Mazila, et al.
Published: (2018)

Isolation, gene expression and structural analysis of fruit bromelain from Ananas comosus cultivar MD 2
by: Pang, Wei Cheng
Published: (2020)

Functional characterization of recombinant bromelain
by: Amid, Azura, et al.
Published: (2015)

Kinetic studies on recombinant stem bromelain
by: Bala, Muntari, et al.
Published: (2013)

Gene expression analysis of fruit bromelain in ripening of Ananas comosus cultivar MD 2
by: Pang, Wei Cheng, et al.
Published: (2020)

Gene Expression Analysis of Fruit Bromelain in Ripening of Ananas comosus Cultivar MD 2
by: Wei, Cheng Pang, et al.
Published: (2020)

Determination of stem bromelain activity from pineapple plant (ananas comosus) / Nurhidayah Mohamad Sait
by: Mohamad Sait, Nurhidayah
Published: (2014)

Differential Scanning Calorimetry as tool in observing thermal and storage stability of recombinant Bromelain
by: Ismail, Nurul Azira, et al.
Published: (2011)

Differential Scanning Calorimetry as tool in observing thermal and storage stability of recombinant Bromelain
by: Ismail, Nurul Azira, et al.
Published: (2012)

Pilot scale extraction of proteolytic enzyme bromelain from pineapple (ananas comosus)
by: Loh, Melissa Pei Shian, et al.
Published: (2005)

Case study: recombinant Bromelain cloning, characterization and upstream processes
by: Amid, Azura, et al.
Published: (2015)

Optimization of spray drying process conditions for recombinant stem bromelain
by: Mohd. Salleh, Hamzah, et al.
Published: (2014)

Case Study: Recombinant Bromelain Selection
by: Amid, Azura, et al.
Published: (2015)

Case Study: Recombinant Bromelain Selection
by: Azura, Amid, et al.
Published: (2015)

Bromelain: an overview of industrial application and purification strategies
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2014)

Bromelain: an Overview of Industrial Application and Purification Strategies
by: Zatul Iffah, Mohd Arshad, et al.
Published: (2014)

Comparison of Purification Methods to Purify Recombinant Bromelain from Escherichia coli BL21-A1
by: Zatul Iffah, Mohd Arshad, et al.
Published: (2017)

Effects of process parameters on spray drying of recombinant bromelain
by: Muntari, Bala, et al.
Published: (2013)

Biosynthesis of nanoparticles using recombinant bromelain
by: Nasruddin, Arina, et al.
Published: (2018)

Bromelain production: Current trends and perspective
by: Bala, Muntari, et al.
Published: (2012)

Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology
by: Muntari, Bala, et al.
Published: (2012)

Gene expression analysis in MCF-7 breast cancer cells treated with recombinant bromelain
by: Fouz, Nour, et al.
Published: (2014)

Heterologous expression of Bromelain in Escherichia coli
by: M., Bala, et al.
Published: (2011)

Pathway analysis of genes affected in MCF-7 breast cancer cells treated with recombinant bromelain
by: Fouz, Nour, et al.
Published: (2013)

Case Study: Recombinant Bromelain Downstream Processing
by: Amid, Azura, et al.
Published: (2015)

Physicochemical properties of Josapine pineapple (Ananas comosus)
by: Mohd Rosdan, Faridatul Ain, et al.
Published: (2012)

Optimization on cell disruption of E. coli BL21-AI expressing recombinant bromelain
by: Othman, Muhd. Ezza Faiez, et al.
Published: (2014)

Recovery of recombinant bromelain from Escherichia coli BL21-AI
by: Bala, Muntari, et al.
Published: (2011)

Comparison of cell disruption method for E. coli BL21-AI expressing recombinant bromelain
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2015)

Utilization of pineapple (Ananas comosus) plant stem as starch and lignocellulose feedstock for biobased products
by: Abd-Aziz, Suraini
Published: (2022)

Effects of operating conditions in spray drying of recombinant bromelain
by: Amid, Azura, et al.
Published: (2014)

Comparison of purification methods to purify recombinant bromelain from Escherichia coli BL21-A1 = Perbandingan kaedah penulenan rekombinan bromelain daripada Escherichia coli BL21-A1
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2017)

Screening of important autoinduction medium composition for high biaomass production of E. coli expressing recombinant bromelain
by: Jamaluddin, Mohd Jamil Aizat, et al.
Published: (2014)

The MD2 'Super Sweet' pineapple (Ananas comosus)
by: Amar, Ahmadi Thalip, et al.
Published: (2015)