Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology
Bromelain, a cysteine protease with various therapeutic and industrial applications, was expressed in Escherichia coli, BL21-AI clone, under different cultivation conditions (post-induction temperature, L-arabinose concentration and post-induction period). The optimized conditions by response surf...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2012
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| Online Access: | http://irep.iium.edu.my/23637/ http://irep.iium.edu.my/23637/1/Bala_final_2191-0855-2-12.pdf |
| _version_ | 1848779187774554112 |
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| author | Muntari, Bala Amid, Azura Jami, Mohammed Saedi Mohd. Salleh, Hamzah Mel, Maizirwan |
| author_facet | Muntari, Bala Amid, Azura Jami, Mohammed Saedi Mohd. Salleh, Hamzah Mel, Maizirwan |
| author_sort | Muntari, Bala |
| building | IIUM Repository |
| collection | Online Access |
| description | Bromelain, a cysteine protease with various therapeutic and industrial applications, was expressed in Escherichia coli,
BL21-AI clone, under different cultivation conditions (post-induction temperature, L-arabinose concentration and
post-induction period). The optimized conditions by response surface methodology using face centered central
composite design were 0.2% (w/v) L-arabinose, 8 hr and 25°C. The analysis of variance coupled with larger value of
R2 (0.989) showed that the quadratic model used for the prediction was highly significant (p < 0.05). Under the
optimized conditions, the model produced bromelain activity of 9.2 U/mg while validation experiments gave
bromelain activity of 9.6 ± 0.02 U/mg at 0.15% (w/v) L-arabinose, 8 hr and 27°C. This study had innovatively
developed cultivation conditions for better production of recombinant bromelain in shake flask culture. |
| first_indexed | 2025-11-14T15:13:48Z |
| format | Article |
| id | iium-23637 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:13:48Z |
| publishDate | 2012 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-236372021-07-14T02:18:05Z http://irep.iium.edu.my/23637/ Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology Muntari, Bala Amid, Azura Jami, Mohammed Saedi Mohd. Salleh, Hamzah Mel, Maizirwan Q Science (General) QR Microbiology Bromelain, a cysteine protease with various therapeutic and industrial applications, was expressed in Escherichia coli, BL21-AI clone, under different cultivation conditions (post-induction temperature, L-arabinose concentration and post-induction period). The optimized conditions by response surface methodology using face centered central composite design were 0.2% (w/v) L-arabinose, 8 hr and 25°C. The analysis of variance coupled with larger value of R2 (0.989) showed that the quadratic model used for the prediction was highly significant (p < 0.05). Under the optimized conditions, the model produced bromelain activity of 9.2 U/mg while validation experiments gave bromelain activity of 9.6 ± 0.02 U/mg at 0.15% (w/v) L-arabinose, 8 hr and 27°C. This study had innovatively developed cultivation conditions for better production of recombinant bromelain in shake flask culture. Springer 2012 Article PeerReviewed application/pdf en http://irep.iium.edu.my/23637/1/Bala_final_2191-0855-2-12.pdf Muntari, Bala and Amid, Azura and Jami, Mohammed Saedi and Mohd. Salleh, Hamzah and Mel, Maizirwan (2012) Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology. AMB Express, 2 (12). pp. 1-9. ISSN 2191-0855 http://www.amb-express.com/content/2/1/12 10.1186/2191-0855-2-12 |
| spellingShingle | Q Science (General) QR Microbiology Muntari, Bala Amid, Azura Jami, Mohammed Saedi Mohd. Salleh, Hamzah Mel, Maizirwan Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology |
| title | Recombinant bromelain production in Escherichia
coli: process optimization in shake flask culture
by response surface methodology |
| title_full | Recombinant bromelain production in Escherichia
coli: process optimization in shake flask culture
by response surface methodology |
| title_fullStr | Recombinant bromelain production in Escherichia
coli: process optimization in shake flask culture
by response surface methodology |
| title_full_unstemmed | Recombinant bromelain production in Escherichia
coli: process optimization in shake flask culture
by response surface methodology |
| title_short | Recombinant bromelain production in Escherichia
coli: process optimization in shake flask culture
by response surface methodology |
| title_sort | recombinant bromelain production in escherichia
coli: process optimization in shake flask culture
by response surface methodology |
| topic | Q Science (General) QR Microbiology |
| url | http://irep.iium.edu.my/23637/ http://irep.iium.edu.my/23637/ http://irep.iium.edu.my/23637/ http://irep.iium.edu.my/23637/1/Bala_final_2191-0855-2-12.pdf |