Isolation and screening of lactic acid bacteria, Lactococcus lactis from Clarias gariepinus (African catfish) with potential use as probiotic in aquaculture
In aquaculture probiotic feeding could play a crucial role in developing microbial control strategies, since disease outbreaks are recognized as important constraints to aquaculture production and the fear of antibiotic resistance. In this study, lactic acid bacteria (LAB) strains from the intestina...
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Academic Journals
2012
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| Online Access: | http://irep.iium.edu.my/23417/ http://irep.iium.edu.my/23417/1/African-catfish_Hamid_et_al.pdf |
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| author | Tengku Abdul Hamid, Tengku Haziyamin Khan Chowdhury, Ahmed Jalal Jalil, Muhammad Fauzi Azhar, Nur Shazana |
| author_facet | Tengku Abdul Hamid, Tengku Haziyamin Khan Chowdhury, Ahmed Jalal Jalil, Muhammad Fauzi Azhar, Nur Shazana |
| author_sort | Tengku Abdul Hamid, Tengku Haziyamin |
| building | IIUM Repository |
| collection | Online Access |
| description | In aquaculture probiotic feeding could play a crucial role in developing microbial control strategies, since disease outbreaks are recognized as important constraints to aquaculture production and the fear of antibiotic resistance. In this study, lactic acid bacteria (LAB) strains from the intestinal tissue of African catfish Clarias griepinus were successfully screened, characterized and identified. The isolates (S1#8, S1#9, S1#10, S1#18, S1#19 S1#20 and S1#21) shared common morphological characteristics of LAB such as non-sporulating, Gram positive cocci or cocco-bacilli shape. These were the dominating morphology found in the catfish, as compared to other types of LABs reported to be found in freshwater fishes. All of the 35 isolates have the ability to utilize lactose as part of their metabolism process and showed negative reactions towards catalase test. These isolates were also tested for antimicrobial activities using disc diffusion assay against indicator Salmonella typhimurium and Escherichia coli. Based on the partial 16S rRNA sequences, the selected LAB isolates belonged to a member of Lactococcus lactis with 98% DNA similarity. This strain can be used as probiotic in aquaculture feeding. |
| first_indexed | 2025-11-14T15:13:14Z |
| format | Article |
| id | iium-23417 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:13:14Z |
| publishDate | 2012 |
| publisher | Academic Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-234172017-12-08T02:27:41Z http://irep.iium.edu.my/23417/ Isolation and screening of lactic acid bacteria, Lactococcus lactis from Clarias gariepinus (African catfish) with potential use as probiotic in aquaculture Tengku Abdul Hamid, Tengku Haziyamin Khan Chowdhury, Ahmed Jalal Jalil, Muhammad Fauzi Azhar, Nur Shazana Q Science (General) In aquaculture probiotic feeding could play a crucial role in developing microbial control strategies, since disease outbreaks are recognized as important constraints to aquaculture production and the fear of antibiotic resistance. In this study, lactic acid bacteria (LAB) strains from the intestinal tissue of African catfish Clarias griepinus were successfully screened, characterized and identified. The isolates (S1#8, S1#9, S1#10, S1#18, S1#19 S1#20 and S1#21) shared common morphological characteristics of LAB such as non-sporulating, Gram positive cocci or cocco-bacilli shape. These were the dominating morphology found in the catfish, as compared to other types of LABs reported to be found in freshwater fishes. All of the 35 isolates have the ability to utilize lactose as part of their metabolism process and showed negative reactions towards catalase test. These isolates were also tested for antimicrobial activities using disc diffusion assay against indicator Salmonella typhimurium and Escherichia coli. Based on the partial 16S rRNA sequences, the selected LAB isolates belonged to a member of Lactococcus lactis with 98% DNA similarity. This strain can be used as probiotic in aquaculture feeding. Academic Journals 2012-04-10 Article PeerReviewed application/pdf en http://irep.iium.edu.my/23417/1/African-catfish_Hamid_et_al.pdf Tengku Abdul Hamid, Tengku Haziyamin and Khan Chowdhury, Ahmed Jalal and Jalil, Muhammad Fauzi and Azhar, Nur Shazana (2012) Isolation and screening of lactic acid bacteria, Lactococcus lactis from Clarias gariepinus (African catfish) with potential use as probiotic in aquaculture. African Journal of Biotechnology, 11 (29). pp. 7494-7499. ISSN 1684–5315 http://academicjournals.org/ajb/abstracts/abs2012/10Apr/Hamid%20et%20al.htm 10.5897/AJB11.3864 |
| spellingShingle | Q Science (General) Tengku Abdul Hamid, Tengku Haziyamin Khan Chowdhury, Ahmed Jalal Jalil, Muhammad Fauzi Azhar, Nur Shazana Isolation and screening of lactic acid bacteria, Lactococcus lactis from Clarias gariepinus (African catfish) with potential use as probiotic in aquaculture |
| title | Isolation and screening of lactic acid bacteria, Lactococcus lactis from Clarias gariepinus (African catfish) with potential use as probiotic in aquaculture |
| title_full | Isolation and screening of lactic acid bacteria, Lactococcus lactis from Clarias gariepinus (African catfish) with potential use as probiotic in aquaculture |
| title_fullStr | Isolation and screening of lactic acid bacteria, Lactococcus lactis from Clarias gariepinus (African catfish) with potential use as probiotic in aquaculture |
| title_full_unstemmed | Isolation and screening of lactic acid bacteria, Lactococcus lactis from Clarias gariepinus (African catfish) with potential use as probiotic in aquaculture |
| title_short | Isolation and screening of lactic acid bacteria, Lactococcus lactis from Clarias gariepinus (African catfish) with potential use as probiotic in aquaculture |
| title_sort | isolation and screening of lactic acid bacteria, lactococcus lactis from clarias gariepinus (african catfish) with potential use as probiotic in aquaculture |
| topic | Q Science (General) |
| url | http://irep.iium.edu.my/23417/ http://irep.iium.edu.my/23417/ http://irep.iium.edu.my/23417/ http://irep.iium.edu.my/23417/1/African-catfish_Hamid_et_al.pdf |