Characterization of purified cellulase from fermentation of sewage sludge
The increasing volume of sewage sludge as well as the municipal and agricultural waste produced and the total managing cost every year has been one of the major environmental issues in Malaysia. The production of cellulase from domestic sewage sludge could possibly bring an economic importance in...
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| Format: | Article |
| Language: | English |
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INSI Publications
2012
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| Online Access: | http://irep.iium.edu.my/23412/ http://irep.iium.edu.my/23412/1/muna_tasnim_et_al.pdf |
| _version_ | 1848779152407134208 |
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| author | Mukhtaruddin, Muna Tasnim Alam, Md Zahangir Mohd. Salleh, Hamzah |
| author_facet | Mukhtaruddin, Muna Tasnim Alam, Md Zahangir Mohd. Salleh, Hamzah |
| author_sort | Mukhtaruddin, Muna Tasnim |
| building | IIUM Repository |
| collection | Online Access |
| description | The increasing volume of sewage sludge as well as the municipal and agricultural waste
produced and the total managing cost every year has been one of the major environmental issues in
Malaysia. The production of cellulase from domestic sewage sludge could possibly bring an economic
importance in certain industries as well as a key to solve the problem of environmental pollution and
also in developing and utilizing renewable energy sources by applying the enzyme on the cellulosic
materials. This study focuses on the characterization of cellulases produced from fermentation broth of
sewage sludge. Using the cellulase enzyme with the activity of 45 U/ml, few tests have been carried out
to characterize and checked the stability of the enzyme. The tests were pH stability, temperature
stability as well as incubation time for the enzyme. Characterization of the enzyme was likely to help
develop efficient application of the enzyme in industrial scale. 50oC was found to be the optimum
temperature for the stability of the enzyme. Besides, pH 3 gave the highest enzyme activity and also 48
hours turn to be the optimum incubation time.
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| first_indexed | 2025-11-14T15:13:14Z |
| format | Article |
| id | iium-23412 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:13:14Z |
| publishDate | 2012 |
| publisher | INSI Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-234122021-07-14T09:23:26Z http://irep.iium.edu.my/23412/ Characterization of purified cellulase from fermentation of sewage sludge Mukhtaruddin, Muna Tasnim Alam, Md Zahangir Mohd. Salleh, Hamzah T175 Industrial research. Research and development TA170 Environmental engineering. Sustainable engineering TP248.13 Biotechnology The increasing volume of sewage sludge as well as the municipal and agricultural waste produced and the total managing cost every year has been one of the major environmental issues in Malaysia. The production of cellulase from domestic sewage sludge could possibly bring an economic importance in certain industries as well as a key to solve the problem of environmental pollution and also in developing and utilizing renewable energy sources by applying the enzyme on the cellulosic materials. This study focuses on the characterization of cellulases produced from fermentation broth of sewage sludge. Using the cellulase enzyme with the activity of 45 U/ml, few tests have been carried out to characterize and checked the stability of the enzyme. The tests were pH stability, temperature stability as well as incubation time for the enzyme. Characterization of the enzyme was likely to help develop efficient application of the enzyme in industrial scale. 50oC was found to be the optimum temperature for the stability of the enzyme. Besides, pH 3 gave the highest enzyme activity and also 48 hours turn to be the optimum incubation time. INSI Publications 2012 Article PeerReviewed application/pdf en http://irep.iium.edu.my/23412/1/muna_tasnim_et_al.pdf Mukhtaruddin, Muna Tasnim and Alam, Md Zahangir and Mohd. Salleh, Hamzah (2012) Characterization of purified cellulase from fermentation of sewage sludge. Australian Journal of Basic and Applied Sciences, 6 (1). pp. 74-78. ISSN 1991-8178 http://www.ajbasweb.com/ajbas/2012/January/74-78.pdf |
| spellingShingle | T175 Industrial research. Research and development TA170 Environmental engineering. Sustainable engineering TP248.13 Biotechnology Mukhtaruddin, Muna Tasnim Alam, Md Zahangir Mohd. Salleh, Hamzah Characterization of purified cellulase from fermentation of sewage sludge |
| title | Characterization of purified cellulase from fermentation of sewage sludge |
| title_full | Characterization of purified cellulase from fermentation of sewage sludge |
| title_fullStr | Characterization of purified cellulase from fermentation of sewage sludge |
| title_full_unstemmed | Characterization of purified cellulase from fermentation of sewage sludge |
| title_short | Characterization of purified cellulase from fermentation of sewage sludge |
| title_sort | characterization of purified cellulase from fermentation of sewage sludge |
| topic | T175 Industrial research. Research and development TA170 Environmental engineering. Sustainable engineering TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/23412/ http://irep.iium.edu.my/23412/ http://irep.iium.edu.my/23412/1/muna_tasnim_et_al.pdf |