A real-time PCR technique in the identification of over-stunned slaughtered chicken

Chickens were water stunned before slaughtered was acceptable in Malaysia under certain condition prepared by MAJLIS FATWA and JAKIM. However, in some country like Brunei this method was not accepted at all. Even in Malaysia, it is unlawful when these chickens were stunned over the current limit...

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Main Authors: Amid, Azura, Ahmad Hassan, Nurhidayah, Abu Samah, Norshahida
Format: Proceeding Paper
Language:English
Published: 2010
Subjects:
Online Access:http://irep.iium.edu.my/22646/
http://irep.iium.edu.my/22646/1/pp79.pdf
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author Amid, Azura
Ahmad Hassan, Nurhidayah
Abu Samah, Norshahida
author_facet Amid, Azura
Ahmad Hassan, Nurhidayah
Abu Samah, Norshahida
author_sort Amid, Azura
building IIUM Repository
collection Online Access
description Chickens were water stunned before slaughtered was acceptable in Malaysia under certain condition prepared by MAJLIS FATWA and JAKIM. However, in some country like Brunei this method was not accepted at all. Even in Malaysia, it is unlawful when these chickens were stunned over the current limit that set by JAKIM. The previous study conducted identified a candidate biomarker that suitable to be used in developing a detection method for over stunned chicken. A real-time PCR technique was applied as a detection method in this study using our identified biomarker in developing a set of specific primers. This study was conducted to observe the effectiveness of a detection method developed for identifying chicken that were stunning over the current limit allowable by JAKIM. Results of this study showed that by applying this detection method we were able to differentiate a chicken that was slaughtered according to original Syara’i method compared to the one that over stunned prior to slaughtering and also able to develop a result within 3 hours.
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format Proceeding Paper
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institution International Islamic University Malaysia
institution_category Local University
language English
last_indexed 2025-11-14T15:11:25Z
publishDate 2010
recordtype eprints
repository_type Digital Repository
spelling iium-226462012-04-10T00:01:26Z http://irep.iium.edu.my/22646/ A real-time PCR technique in the identification of over-stunned slaughtered chicken Amid, Azura Ahmad Hassan, Nurhidayah Abu Samah, Norshahida TJ Mechanical engineering and machinery Chickens were water stunned before slaughtered was acceptable in Malaysia under certain condition prepared by MAJLIS FATWA and JAKIM. However, in some country like Brunei this method was not accepted at all. Even in Malaysia, it is unlawful when these chickens were stunned over the current limit that set by JAKIM. The previous study conducted identified a candidate biomarker that suitable to be used in developing a detection method for over stunned chicken. A real-time PCR technique was applied as a detection method in this study using our identified biomarker in developing a set of specific primers. This study was conducted to observe the effectiveness of a detection method developed for identifying chicken that were stunning over the current limit allowable by JAKIM. Results of this study showed that by applying this detection method we were able to differentiate a chicken that was slaughtered according to original Syara’i method compared to the one that over stunned prior to slaughtering and also able to develop a result within 3 hours. 2010 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/22646/1/pp79.pdf Amid, Azura and Ahmad Hassan, Nurhidayah and Abu Samah, Norshahida (2010) A real-time PCR technique in the identification of over-stunned slaughtered chicken. In: IIUM Research, Innovation & Invention Exhibition (IRIIE 2010), 26 - 27 January 2010, Kuala Lumpur.
spellingShingle TJ Mechanical engineering and machinery
Amid, Azura
Ahmad Hassan, Nurhidayah
Abu Samah, Norshahida
A real-time PCR technique in the identification of over-stunned slaughtered chicken
title A real-time PCR technique in the identification of over-stunned slaughtered chicken
title_full A real-time PCR technique in the identification of over-stunned slaughtered chicken
title_fullStr A real-time PCR technique in the identification of over-stunned slaughtered chicken
title_full_unstemmed A real-time PCR technique in the identification of over-stunned slaughtered chicken
title_short A real-time PCR technique in the identification of over-stunned slaughtered chicken
title_sort real-time pcr technique in the identification of over-stunned slaughtered chicken
topic TJ Mechanical engineering and machinery
url http://irep.iium.edu.my/22646/
http://irep.iium.edu.my/22646/1/pp79.pdf