The isolation and characterization of coagulasenegative Staphylococcus sp. from intestine of a Malaysian rabbit (Oryctolagus cuniculus)
The isolation and characterization of lactic acid bacteria (LAB) from the intestine of Oryctolagus cuniculus, a domestic rabbit species in Malaysia is described. Fifty isolates from rabbit intestine were screened by biochemical tests. From 50 isolates, four were identified and shown to be catalase-p...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Faculty of Resource Science and Technology, Universiti Malaysia Sarawak
2012
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/22532/ http://irep.iium.edu.my/22532/1/BJRST_020_201_version3.pdf |
| _version_ | 1848779017612689408 |
|---|---|
| author | Tengku Abdul Hamid, Tengku Haziyamin Mazlan, Farhaneen Afzal |
| author_facet | Tengku Abdul Hamid, Tengku Haziyamin Mazlan, Farhaneen Afzal |
| author_sort | Tengku Abdul Hamid, Tengku Haziyamin |
| building | IIUM Repository |
| collection | Online Access |
| description | The isolation and characterization of lactic acid bacteria (LAB) from the intestine of Oryctolagus cuniculus, a domestic rabbit species in Malaysia is described. Fifty isolates from rabbit intestine were screened by biochemical tests. From 50 isolates, four were identified and shown to be catalase-positive, lactose positive and Gram-positive cocci. Antibacterial assays were carried out against Escherichia coli and Salmonella enteritidis as indicator bacteria. The samples exhibited antibacterial properties as indicated by zone of inhibitions. Three isolates were further subjected to 16S ribosomal RNA (rRNA) gene sequencing and analysis. Partial 16SrRNA sequencing results from these isolates showed high sequence similarity with coagulase negative Staphylococcus sp. This result showed that by screening of LAB from rabbit intestine, bacteria from the family Staphylococcaceae could be isolated and this could be potentially used as probiotics in rabbit feeding.
|
| first_indexed | 2025-11-14T15:11:05Z |
| format | Article |
| id | iium-22532 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:11:05Z |
| publishDate | 2012 |
| publisher | Faculty of Resource Science and Technology, Universiti Malaysia Sarawak |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-225322012-09-26T00:29:13Z http://irep.iium.edu.my/22532/ The isolation and characterization of coagulasenegative Staphylococcus sp. from intestine of a Malaysian rabbit (Oryctolagus cuniculus) Tengku Abdul Hamid, Tengku Haziyamin Mazlan, Farhaneen Afzal Q Science (General) The isolation and characterization of lactic acid bacteria (LAB) from the intestine of Oryctolagus cuniculus, a domestic rabbit species in Malaysia is described. Fifty isolates from rabbit intestine were screened by biochemical tests. From 50 isolates, four were identified and shown to be catalase-positive, lactose positive and Gram-positive cocci. Antibacterial assays were carried out against Escherichia coli and Salmonella enteritidis as indicator bacteria. The samples exhibited antibacterial properties as indicated by zone of inhibitions. Three isolates were further subjected to 16S ribosomal RNA (rRNA) gene sequencing and analysis. Partial 16SrRNA sequencing results from these isolates showed high sequence similarity with coagulase negative Staphylococcus sp. This result showed that by screening of LAB from rabbit intestine, bacteria from the family Staphylococcaceae could be isolated and this could be potentially used as probiotics in rabbit feeding. Faculty of Resource Science and Technology, Universiti Malaysia Sarawak 2012 Article PeerReviewed application/pdf en http://irep.iium.edu.my/22532/1/BJRST_020_201_version3.pdf Tengku Abdul Hamid, Tengku Haziyamin and Mazlan, Farhaneen Afzal (2012) The isolation and characterization of coagulasenegative Staphylococcus sp. from intestine of a Malaysian rabbit (Oryctolagus cuniculus). Borneo Journal of Resource Science and Technology, 2 (1). pp. 54-58. ISSN 229-9769 http://www.frst.unimas.my/bjrst-online-issue.html |
| spellingShingle | Q Science (General) Tengku Abdul Hamid, Tengku Haziyamin Mazlan, Farhaneen Afzal The isolation and characterization of coagulasenegative Staphylococcus sp. from intestine of a Malaysian rabbit (Oryctolagus cuniculus) |
| title | The isolation and characterization of coagulasenegative
Staphylococcus sp. from intestine of a Malaysian
rabbit (Oryctolagus cuniculus)
|
| title_full | The isolation and characterization of coagulasenegative
Staphylococcus sp. from intestine of a Malaysian
rabbit (Oryctolagus cuniculus)
|
| title_fullStr | The isolation and characterization of coagulasenegative
Staphylococcus sp. from intestine of a Malaysian
rabbit (Oryctolagus cuniculus)
|
| title_full_unstemmed | The isolation and characterization of coagulasenegative
Staphylococcus sp. from intestine of a Malaysian
rabbit (Oryctolagus cuniculus)
|
| title_short | The isolation and characterization of coagulasenegative
Staphylococcus sp. from intestine of a Malaysian
rabbit (Oryctolagus cuniculus)
|
| title_sort | isolation and characterization of coagulasenegative
staphylococcus sp. from intestine of a malaysian
rabbit (oryctolagus cuniculus) |
| topic | Q Science (General) |
| url | http://irep.iium.edu.my/22532/ http://irep.iium.edu.my/22532/ http://irep.iium.edu.my/22532/1/BJRST_020_201_version3.pdf |