Effects of cell immobilization to the phytate-degrading enzyme activity
The use of immobilized whole microhial cells and/or organelles eliminates lhc often tedious. lime consuming. and expensi\'e sleps invohed in isolation and purificatioll of imraccllular enzymes. II al>o lends 10 enhance the stability of lhe enzyme by relaining its nall.....l catalytic surroun...
| Main Authors: | , , |
|---|---|
| Format: | Book Chapter |
| Language: | English |
| Published: |
IIUM Press
2011
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/20776/ http://irep.iium.edu.my/20776/1/EFFECTS_OF_CELL_IMMOBILIZATION_TO_THE_PHYTATE-_DEGRADING_ENZYME_ACTNITY.pdf |
| _version_ | 1848778663236993024 |
|---|---|
| author | Mohamad Puad, Noor Illi Farouk, Abd Alaziem Salleh, Hamzah Mohd. |
| author_facet | Mohamad Puad, Noor Illi Farouk, Abd Alaziem Salleh, Hamzah Mohd. |
| author_sort | Mohamad Puad, Noor Illi |
| building | IIUM Repository |
| collection | Online Access |
| description | The use of immobilized whole microhial cells and/or organelles eliminates lhc often tedious. lime consuming. and expensi\'e sleps invohed in isolation and purificatioll of imraccllular enzymes. II al>o lends 10 enhance the stability of lhe enzyme by relaining its nall.....l catalytic surroundings during immobilizalion and subsequent cominuous operalion. A baclerium isolated from
Malaysian waslewatn pnxlucing a 1l0,-e1 phyrate-degrading enzyme namely PhyFAUIAI was immobilized in alginate gel beads. PhyFAUIA 1 is a periplasmatic enzyme and highly substralespecific 10 phytale. The enzyme aClivity wa~ invesligaled upon cell immobilization. No shift in
pH oplima of phytase of immobilized cells was obsen·ed. 110wever. the optimum lcmperaturcbwas shifted 10 50°C, which was 15°C lower lhan free PhyFAUIAI enzyme. Immobilized cells were found 10 be more stable in wider range of pll and tcmpe-ramr.: compared to free cells. The
results presenled in this work show lhe potential for using immobilized cells to produce ditrerent "~nJ-inosilOl phosphates intennediales |
| first_indexed | 2025-11-14T15:05:27Z |
| format | Book Chapter |
| id | iium-20776 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:05:27Z |
| publishDate | 2011 |
| publisher | IIUM Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-207762021-06-14T13:33:11Z http://irep.iium.edu.my/20776/ Effects of cell immobilization to the phytate-degrading enzyme activity Mohamad Puad, Noor Illi Farouk, Abd Alaziem Salleh, Hamzah Mohd. TP248.13 Biotechnology The use of immobilized whole microhial cells and/or organelles eliminates lhc often tedious. lime consuming. and expensi\'e sleps invohed in isolation and purificatioll of imraccllular enzymes. II al>o lends 10 enhance the stability of lhe enzyme by relaining its nall.....l catalytic surroundings during immobilizalion and subsequent cominuous operalion. A baclerium isolated from Malaysian waslewatn pnxlucing a 1l0,-e1 phyrate-degrading enzyme namely PhyFAUIAI was immobilized in alginate gel beads. PhyFAUIA 1 is a periplasmatic enzyme and highly substralespecific 10 phytale. The enzyme aClivity wa~ invesligaled upon cell immobilization. No shift in pH oplima of phytase of immobilized cells was obsen·ed. 110wever. the optimum lcmperaturcbwas shifted 10 50°C, which was 15°C lower lhan free PhyFAUIAI enzyme. Immobilized cells were found 10 be more stable in wider range of pll and tcmpe-ramr.: compared to free cells. The results presenled in this work show lhe potential for using immobilized cells to produce ditrerent "~nJ-inosilOl phosphates intennediales IIUM Press 2011 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/20776/1/EFFECTS_OF_CELL_IMMOBILIZATION_TO_THE_PHYTATE-_DEGRADING_ENZYME_ACTNITY.pdf Mohamad Puad, Noor Illi and Farouk, Abd Alaziem and Salleh, Hamzah Mohd. (2011) Effects of cell immobilization to the phytate-degrading enzyme activity. In: Current Research and Development in Biotechnology Engineering at IIUM (volume II). IIUM Press, Kuala Lumpur, pp. 137-143. ISBN 978-967-418-151-2 http://rms.research.iium.edu.my/bookstore/default.aspx |
| spellingShingle | TP248.13 Biotechnology Mohamad Puad, Noor Illi Farouk, Abd Alaziem Salleh, Hamzah Mohd. Effects of cell immobilization to the phytate-degrading enzyme activity |
| title | Effects of cell immobilization to the phytate-degrading enzyme activity |
| title_full | Effects of cell immobilization to the phytate-degrading enzyme activity |
| title_fullStr | Effects of cell immobilization to the phytate-degrading enzyme activity |
| title_full_unstemmed | Effects of cell immobilization to the phytate-degrading enzyme activity |
| title_short | Effects of cell immobilization to the phytate-degrading enzyme activity |
| title_sort | effects of cell immobilization to the phytate-degrading enzyme activity |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/20776/ http://irep.iium.edu.my/20776/ http://irep.iium.edu.my/20776/1/EFFECTS_OF_CELL_IMMOBILIZATION_TO_THE_PHYTATE-_DEGRADING_ENZYME_ACTNITY.pdf |