Biotransfomation products from Clarius batrachus oil
Biotransformation can be defined as an application that utilized natural and recombinant organisms’ enzymes secreted by yeast, fungi and bacteria or whole cells as catalyst in synthesis of organic compound. Therefore, Pseudomonas aeruginosa bacterium has been chosen to be the biocatalyst for biotran...
| Main Authors: | , , , , |
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| Format: | Proceeding Paper |
| Language: | English English |
| Published: |
2011
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| Online Access: | http://irep.iium.edu.my/17290/ http://irep.iium.edu.my/17290/1/IRIE_2011_Front_page_.PDF http://irep.iium.edu.my/17290/3/Biotransformation_product_catfish_oil.pdf |
| _version_ | 1848778020662280192 |
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| author | Omar, Muhammad Nor Shaban, Norhayati Bakar, Latifah Munirah Zuberdi, Ahmad Muzammil Yunus, Kamaruzzaman |
| author_facet | Omar, Muhammad Nor Shaban, Norhayati Bakar, Latifah Munirah Zuberdi, Ahmad Muzammil Yunus, Kamaruzzaman |
| author_sort | Omar, Muhammad Nor |
| building | IIUM Repository |
| collection | Online Access |
| description | Biotransformation can be defined as an application that utilized natural and recombinant organisms’ enzymes secreted by yeast, fungi and bacteria or whole cells as catalyst in synthesis of organic compound. Therefore, Pseudomonas aeruginosa bacterium has been chosen to be the biocatalyst for biotransformation of ω fatty acids extracted from Malaysian catfish, Clarias batrachus emphasizing on bioconversion of arachidonic acid. In addition, arachidonic acid was one of prostaglandin precursor which exerts a variety of pharmacological effects on human and animals. In this study, the fatty acids were extracted from the catfish using modified Folch method where the fish flesh was freeze dried prior to homogenization with the chloroform and methanol system. Then, the crude lipid extract was added to the bacterial culture and incubated for 4 days. After incubation, the biotransformation product was extracted and analyzed by using gas chromatography and mass spectrometer (GC-MS) to identify the fatty acids and other compounds. It was found that several fatty acids, especially ω-fatty acids were converted to cholesterol. This indicates that ω-fatty acids can be used as starting materials for other bioactive metabolites for pharmaceutical purposes. |
| first_indexed | 2025-11-14T14:55:14Z |
| format | Proceeding Paper |
| id | iium-17290 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T14:55:14Z |
| publishDate | 2011 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-172902012-03-08T01:31:26Z http://irep.iium.edu.my/17290/ Biotransfomation products from Clarius batrachus oil Omar, Muhammad Nor Shaban, Norhayati Bakar, Latifah Munirah Zuberdi, Ahmad Muzammil Yunus, Kamaruzzaman Q Science (General) Biotransformation can be defined as an application that utilized natural and recombinant organisms’ enzymes secreted by yeast, fungi and bacteria or whole cells as catalyst in synthesis of organic compound. Therefore, Pseudomonas aeruginosa bacterium has been chosen to be the biocatalyst for biotransformation of ω fatty acids extracted from Malaysian catfish, Clarias batrachus emphasizing on bioconversion of arachidonic acid. In addition, arachidonic acid was one of prostaglandin precursor which exerts a variety of pharmacological effects on human and animals. In this study, the fatty acids were extracted from the catfish using modified Folch method where the fish flesh was freeze dried prior to homogenization with the chloroform and methanol system. Then, the crude lipid extract was added to the bacterial culture and incubated for 4 days. After incubation, the biotransformation product was extracted and analyzed by using gas chromatography and mass spectrometer (GC-MS) to identify the fatty acids and other compounds. It was found that several fatty acids, especially ω-fatty acids were converted to cholesterol. This indicates that ω-fatty acids can be used as starting materials for other bioactive metabolites for pharmaceutical purposes. 2011-02-09 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/17290/1/IRIE_2011_Front_page_.PDF application/pdf en http://irep.iium.edu.my/17290/3/Biotransformation_product_catfish_oil.pdf Omar, Muhammad Nor and Shaban, Norhayati and Bakar, Latifah Munirah and Zuberdi, Ahmad Muzammil and Yunus, Kamaruzzaman (2011) Biotransfomation products from Clarius batrachus oil. In: IIUM Research, Invention and Innovation Exhibition (IRIIE) 2011, 9-10 February 2011, Cultural Activity Centre (CAC) and KAED Gallery IIUM. |
| spellingShingle | Q Science (General) Omar, Muhammad Nor Shaban, Norhayati Bakar, Latifah Munirah Zuberdi, Ahmad Muzammil Yunus, Kamaruzzaman Biotransfomation products from Clarius batrachus oil |
| title | Biotransfomation products from Clarius batrachus oil |
| title_full | Biotransfomation products from Clarius batrachus oil |
| title_fullStr | Biotransfomation products from Clarius batrachus oil |
| title_full_unstemmed | Biotransfomation products from Clarius batrachus oil |
| title_short | Biotransfomation products from Clarius batrachus oil |
| title_sort | biotransfomation products from clarius batrachus oil |
| topic | Q Science (General) |
| url | http://irep.iium.edu.my/17290/ http://irep.iium.edu.my/17290/1/IRIE_2011_Front_page_.PDF http://irep.iium.edu.my/17290/3/Biotransformation_product_catfish_oil.pdf |