Molecular cloning and production of recombinant phytase from Bacillus Subtilis ASUIA243 in Pichia Pastoris

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Molecular cloning and production of recombinant phytase from Bacillus Subtilis ASUIA243 in Pichia Pastoris

Phytase gene obtained from Bacillus subtilis ASUIA243 was cloned into a medium vector and transformed into E. coli. Restriction enzyme digestion was conducted to get blunt-ended phytase gene and ligated into the Pichia expression vector, pPICZαA. The recombinant vector, pPICZαA-243HPp was then linea...

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Bibliographic Details
Main Authors:	Mohd Dali, Nor Soleha, Nuge, Tamrin, Mahamad Maifiah, Mohd Hafidz, Yusof, Faridah, Meor Hussin, Anis Shobirin, Farouk, Abd Alaziem, Mohd. Salleh, Hamzah
Format:	Article
Language:	English
Published:	IIUM Press 2011
Subjects:	QH426 Genetics
Online Access:	http://irep.iium.edu.my/17110/ http://irep.iium.edu.my/17110/1/molecular_cloning.pdf

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