Some biological activities of Malaysian leech salive extract

Leeches were fed on the phagostimulatory solution through parafilm membrane. The satiated leeches were forced to regurgitate the solution by soaking them in an ice-container. The anticoagulant activity was ascertained using thrombin time assay (TT). The result revealed that the saliva concentration...

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Main Authors: M.Abdualkader, Abdualrahman, Merzouk, Ahmed, Mohammed Ghawi, Abbas, Alaama, Mohammed
Format: Article
Language:English
Published: IIUM Press 2011
Subjects:
Online Access:http://irep.iium.edu.my/16870/
http://irep.iium.edu.my/16870/1/some_biological.pdf
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author M.Abdualkader, Abdualrahman
Merzouk, Ahmed
Mohammed Ghawi, Abbas
Alaama, Mohammed
author_facet M.Abdualkader, Abdualrahman
Merzouk, Ahmed
Mohammed Ghawi, Abbas
Alaama, Mohammed
author_sort M.Abdualkader, Abdualrahman
building IIUM Repository
collection Online Access
description Leeches were fed on the phagostimulatory solution through parafilm membrane. The satiated leeches were forced to regurgitate the solution by soaking them in an ice-container. The anticoagulant activity was ascertained using thrombin time assay (TT). The result revealed that the saliva concentration which increases TT by 100% (IC100) is 43.205 μg/ml plasma. The antimicrobial activity of the saliva was tested against several bacterial spp. (E. coli, P. aeruginosa, B. cereus, Sal. typhi and S. aureus) and fungi spp. (C.albicans and C.neoformans). It was found that saliva has an inhibition activity against Sal. typhi (minimal inhibitory concentration MIC 78.253 μg/ml), S.aureus (MIC 78.253 μg/ml) and E .coli (MIC 121.256 μg/ml).
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institution International Islamic University Malaysia
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spelling iium-168702012-01-25T10:36:19Z http://irep.iium.edu.my/16870/ Some biological activities of Malaysian leech salive extract M.Abdualkader, Abdualrahman Merzouk, Ahmed Mohammed Ghawi, Abbas Alaama, Mohammed R Medicine (General) Leeches were fed on the phagostimulatory solution through parafilm membrane. The satiated leeches were forced to regurgitate the solution by soaking them in an ice-container. The anticoagulant activity was ascertained using thrombin time assay (TT). The result revealed that the saliva concentration which increases TT by 100% (IC100) is 43.205 μg/ml plasma. The antimicrobial activity of the saliva was tested against several bacterial spp. (E. coli, P. aeruginosa, B. cereus, Sal. typhi and S. aureus) and fungi spp. (C.albicans and C.neoformans). It was found that saliva has an inhibition activity against Sal. typhi (minimal inhibitory concentration MIC 78.253 μg/ml), S.aureus (MIC 78.253 μg/ml) and E .coli (MIC 121.256 μg/ml). IIUM Press 2011 Article PeerReviewed application/pdf en http://irep.iium.edu.my/16870/1/some_biological.pdf M.Abdualkader, Abdualrahman and Merzouk, Ahmed and Mohammed Ghawi, Abbas and Alaama, Mohammed (2011) Some biological activities of Malaysian leech salive extract. IIUM Engineering Journal, 12 (4). pp. 1-9. ISSN 1511-788X http://www.iium.edu.my/ejournal/index.php/iiumej/article/view/156/196
spellingShingle R Medicine (General)
M.Abdualkader, Abdualrahman
Merzouk, Ahmed
Mohammed Ghawi, Abbas
Alaama, Mohammed
Some biological activities of Malaysian leech salive extract
title Some biological activities of Malaysian leech salive extract
title_full Some biological activities of Malaysian leech salive extract
title_fullStr Some biological activities of Malaysian leech salive extract
title_full_unstemmed Some biological activities of Malaysian leech salive extract
title_short Some biological activities of Malaysian leech salive extract
title_sort some biological activities of malaysian leech salive extract
topic R Medicine (General)
url http://irep.iium.edu.my/16870/
http://irep.iium.edu.my/16870/
http://irep.iium.edu.my/16870/1/some_biological.pdf