Isolation and screening of potential microorganisms for citric acid production from sewage treatment plant sludge

The better utilization of sewage treatment (STP) sludge through value added products is being considered to be an alternative cost effective solution for waste management. The study emphasized on isolation of filamentous fungi especially Aspergillus from STP sludge for better adaptability. Liquid st...

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Main Authors: Jamal, Parveen, Alam, Md. Zahangir, Mohd Salleh, Mohammad Ramlan, M. Akib, Munirah
Format: Proceeding Paper
Language:English
Published: 2005
Subjects:
Online Access:http://irep.iium.edu.my/16818/
http://irep.iium.edu.my/16818/1/ABS_2005_Citric_acid.pdf
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author Jamal, Parveen
Alam, Md. Zahangir
Mohd Salleh, Mohammad Ramlan
M. Akib, Munirah
author_facet Jamal, Parveen
Alam, Md. Zahangir
Mohd Salleh, Mohammad Ramlan
M. Akib, Munirah
author_sort Jamal, Parveen
building IIUM Repository
collection Online Access
description The better utilization of sewage treatment (STP) sludge through value added products is being considered to be an alternative cost effective solution for waste management. The study emphasized on isolation of filamentous fungi especially Aspergillus from STP sludge for better adaptability. Liquid state fermentation process was used forthe production of citric acid. Six strains of Aspergillus niger (A-SS101, A-SS102, A-SS104, A-SS105, A-SS106 and A-SS-107) were isolated from STP sludge and identified using slide culture technique followed by image analysis. Four strains were screened under controlled fermentation conditions such as pH range of 2-3, temperature 30 degree celcius and agitation 150 rpm, using the substrate of STP sludge (1% of total suspended solids, TSS), 2% (w/v) co-substrate (wheat flour) with inoculum's size of 2% (10^8 spores/ml). Evaluation of fungal potentiality was done in terms of maximum citric acid production, biosolid production (TSS %) and removal of chemical oxygen demand (COD). Strain A-SS106 produced the highest concentration of citric acid (0.14 g/L), TSS (15.18 g/L) and COD removal (90.1%) followed by SC906, A-SS106, A1020 and A103 on fourth day of fermentation.
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format Proceeding Paper
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institution International Islamic University Malaysia
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language English
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spelling iium-168182024-12-24T07:34:14Z http://irep.iium.edu.my/16818/ Isolation and screening of potential microorganisms for citric acid production from sewage treatment plant sludge Jamal, Parveen Alam, Md. Zahangir Mohd Salleh, Mohammad Ramlan M. Akib, Munirah TA170 Environmental engineering. Sustainable engineering TD Environmental technology. Sanitary engineering TP248.13 Biotechnology The better utilization of sewage treatment (STP) sludge through value added products is being considered to be an alternative cost effective solution for waste management. The study emphasized on isolation of filamentous fungi especially Aspergillus from STP sludge for better adaptability. Liquid state fermentation process was used forthe production of citric acid. Six strains of Aspergillus niger (A-SS101, A-SS102, A-SS104, A-SS105, A-SS106 and A-SS-107) were isolated from STP sludge and identified using slide culture technique followed by image analysis. Four strains were screened under controlled fermentation conditions such as pH range of 2-3, temperature 30 degree celcius and agitation 150 rpm, using the substrate of STP sludge (1% of total suspended solids, TSS), 2% (w/v) co-substrate (wheat flour) with inoculum's size of 2% (10^8 spores/ml). Evaluation of fungal potentiality was done in terms of maximum citric acid production, biosolid production (TSS %) and removal of chemical oxygen demand (COD). Strain A-SS106 produced the highest concentration of citric acid (0.14 g/L), TSS (15.18 g/L) and COD removal (90.1%) followed by SC906, A-SS106, A1020 and A103 on fourth day of fermentation. 2005-06-22 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/16818/1/ABS_2005_Citric_acid.pdf Jamal, Parveen and Alam, Md. Zahangir and Mohd Salleh, Mohammad Ramlan and M. Akib, Munirah (2005) Isolation and screening of potential microorganisms for citric acid production from sewage treatment plant sludge. In: The 8th Applied Biology Symposium 2005, 22nd - 23rd June 2005, Marriot, Putrajaya.
spellingShingle TA170 Environmental engineering. Sustainable engineering
TD Environmental technology. Sanitary engineering
TP248.13 Biotechnology
Jamal, Parveen
Alam, Md. Zahangir
Mohd Salleh, Mohammad Ramlan
M. Akib, Munirah
Isolation and screening of potential microorganisms for citric acid production from sewage treatment plant sludge
title Isolation and screening of potential microorganisms for citric acid production from sewage treatment plant sludge
title_full Isolation and screening of potential microorganisms for citric acid production from sewage treatment plant sludge
title_fullStr Isolation and screening of potential microorganisms for citric acid production from sewage treatment plant sludge
title_full_unstemmed Isolation and screening of potential microorganisms for citric acid production from sewage treatment plant sludge
title_short Isolation and screening of potential microorganisms for citric acid production from sewage treatment plant sludge
title_sort isolation and screening of potential microorganisms for citric acid production from sewage treatment plant sludge
topic TA170 Environmental engineering. Sustainable engineering
TD Environmental technology. Sanitary engineering
TP248.13 Biotechnology
url http://irep.iium.edu.my/16818/
http://irep.iium.edu.my/16818/1/ABS_2005_Citric_acid.pdf