Enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme

The rapid development and the economic growth of oil palm industry have exacerbated environmental pollution since it results in the large quantity of waste products produced from the oil extraction process. In many cases, however, low catalytic efficiency and instability of enzymes were considered a...

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Main Authors: Mohammed Abd., Rasha, Al-Khatib, Ma'an Fahmi Rashid, Alam, Md. Zahangir, Mohd. Salleh, Hamzah
Format: Proceeding Paper
Language:English
Published: 2011
Subjects:
Online Access:http://irep.iium.edu.my/16740/
http://irep.iium.edu.my/16740/1/enzymatic_hydrolysis_of_oil_palm_empty_fruit_bunch_using.pdf
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author Mohammed Abd., Rasha
Al-Khatib, Ma'an Fahmi Rashid
Alam, Md. Zahangir
Mohd. Salleh, Hamzah
author_facet Mohammed Abd., Rasha
Al-Khatib, Ma'an Fahmi Rashid
Alam, Md. Zahangir
Mohd. Salleh, Hamzah
author_sort Mohammed Abd., Rasha
building IIUM Repository
collection Online Access
description The rapid development and the economic growth of oil palm industry have exacerbated environmental pollution since it results in the large quantity of waste products produced from the oil extraction process. In many cases, however, low catalytic efficiency and instability of enzymes were considered as barriers for the development of large-scale operations and applications. In this study, hydrolysis of Oil Palm Empty Fruit Bunch to reducing sugar was achieved using cellulase enzyme immobilized on multi-wall carbon nanotubes. The hydrolysis evaluation was performed using free cellulase enzyme. The amount of reducing sugar concentration released during the reaction was determined using Dinitrosalicylic acid (DNS) assay. Using the immobilized form of the enzyme, it was found that the reducing sugar concentration was increased and the enzymatic hydrolysis of Oil Palm Empty Fruit Bunch was enhanced greatly over long time of hydrolysis.
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format Proceeding Paper
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institution International Islamic University Malaysia
institution_category Local University
language English
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publishDate 2011
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repository_type Digital Repository
spelling iium-167402024-05-06T07:22:22Z http://irep.iium.edu.my/16740/ Enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme Mohammed Abd., Rasha Al-Khatib, Ma'an Fahmi Rashid Alam, Md. Zahangir Mohd. Salleh, Hamzah TP248.13 Biotechnology The rapid development and the economic growth of oil palm industry have exacerbated environmental pollution since it results in the large quantity of waste products produced from the oil extraction process. In many cases, however, low catalytic efficiency and instability of enzymes were considered as barriers for the development of large-scale operations and applications. In this study, hydrolysis of Oil Palm Empty Fruit Bunch to reducing sugar was achieved using cellulase enzyme immobilized on multi-wall carbon nanotubes. The hydrolysis evaluation was performed using free cellulase enzyme. The amount of reducing sugar concentration released during the reaction was determined using Dinitrosalicylic acid (DNS) assay. Using the immobilized form of the enzyme, it was found that the reducing sugar concentration was increased and the enzymatic hydrolysis of Oil Palm Empty Fruit Bunch was enhanced greatly over long time of hydrolysis. 2011-05 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/16740/1/enzymatic_hydrolysis_of_oil_palm_empty_fruit_bunch_using.pdf Mohammed Abd., Rasha and Al-Khatib, Ma'an Fahmi Rashid and Alam, Md. Zahangir and Mohd. Salleh, Hamzah (2011) Enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/
spellingShingle TP248.13 Biotechnology
Mohammed Abd., Rasha
Al-Khatib, Ma'an Fahmi Rashid
Alam, Md. Zahangir
Mohd. Salleh, Hamzah
Enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme
title Enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme
title_full Enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme
title_fullStr Enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme
title_full_unstemmed Enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme
title_short Enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme
title_sort enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme
topic TP248.13 Biotechnology
url http://irep.iium.edu.my/16740/
http://irep.iium.edu.my/16740/
http://irep.iium.edu.my/16740/1/enzymatic_hydrolysis_of_oil_palm_empty_fruit_bunch_using.pdf