Regeneration of Jatropha curcas using leaf explants
A tissue culture protocol for regeneration of Jatropha curcas using leaf explants via somatic embryogenesis was established. The best response for callus induction from leaf explants was on Murashige and Skoog (MS) medium containing BA (1.0 and 2.0 mg l-1) and IBA (1.0 mg l-1). Callus was maintained...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2010
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| Online Access: | http://irep.iium.edu.my/15906/ http://irep.iium.edu.my/15906/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf |
| _version_ | 1848777854710448128 |
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| author | Pappusamy, Arokiaraj S.A, Lydia L., Norshahidah K, Latiffah |
| author_facet | Pappusamy, Arokiaraj S.A, Lydia L., Norshahidah K, Latiffah |
| author_sort | Pappusamy, Arokiaraj |
| building | IIUM Repository |
| collection | Online Access |
| description | A tissue culture protocol for regeneration of Jatropha curcas using leaf explants via somatic embryogenesis was established. The best response for callus induction from leaf explants was on Murashige and Skoog (MS) medium containing BA (1.0 and 2.0 mg l-1) and IBA (1.0 mg l-1). Callus was maintained in light and dark on MS medium supplemented with 0.5 mg l-1 BA and 0.5 mg l-1 IBA.
The best response for shoot induction and shoot multiplication was on treatments containing (1.0 – 2.0 mg
l-1) BA. The shoots were elongated and rooted on ½ strength MS medium containing 1.0 mg l-1 NAA (Napthalene Acetic Acid). |
| first_indexed | 2025-11-14T14:52:36Z |
| format | Proceeding Paper |
| id | iium-15906 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:52:36Z |
| publishDate | 2010 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-159062012-01-30T01:46:17Z http://irep.iium.edu.my/15906/ Regeneration of Jatropha curcas using leaf explants Pappusamy, Arokiaraj S.A, Lydia L., Norshahidah K, Latiffah TP248.13 Biotechnology A tissue culture protocol for regeneration of Jatropha curcas using leaf explants via somatic embryogenesis was established. The best response for callus induction from leaf explants was on Murashige and Skoog (MS) medium containing BA (1.0 and 2.0 mg l-1) and IBA (1.0 mg l-1). Callus was maintained in light and dark on MS medium supplemented with 0.5 mg l-1 BA and 0.5 mg l-1 IBA. The best response for shoot induction and shoot multiplication was on treatments containing (1.0 – 2.0 mg l-1) BA. The shoots were elongated and rooted on ½ strength MS medium containing 1.0 mg l-1 NAA (Napthalene Acetic Acid). 2010 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/15906/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf Pappusamy, Arokiaraj and S.A, Lydia and L., Norshahidah and K, Latiffah (2010) Regeneration of Jatropha curcas using leaf explants. In: IIUM Research, Innovation & Invention Exhibition (IRIIE 2010), 26 - 27 January 2010, Kuala Lumpur. http://www.iium.edu.my/irie/10/info/Programme_Book%20Part_2.pdf |
| spellingShingle | TP248.13 Biotechnology Pappusamy, Arokiaraj S.A, Lydia L., Norshahidah K, Latiffah Regeneration of Jatropha curcas using leaf explants |
| title | Regeneration of Jatropha curcas using leaf explants |
| title_full | Regeneration of Jatropha curcas using leaf explants |
| title_fullStr | Regeneration of Jatropha curcas using leaf explants |
| title_full_unstemmed | Regeneration of Jatropha curcas using leaf explants |
| title_short | Regeneration of Jatropha curcas using leaf explants |
| title_sort | regeneration of jatropha curcas using leaf explants |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/15906/ http://irep.iium.edu.my/15906/ http://irep.iium.edu.my/15906/1/2010_IRIIE_Kamarul_et_al_Aromatic.pdf |