Recovery of recombinant bromelain from Escherichia coli BL21-AI
In spite of the fact that commercial bromelain supplements are available in the market, to date, none of them are produced and formulated from recombinant forms. They are extracted and purified (often partially) from the stem and fruit of pineapple (Ananas comosus). This makes the production of brom...
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| Format: | Article |
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Academic Journals
2011
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| Online Access: | http://irep.iium.edu.my/14293/ http://irep.iium.edu.my/14293/1/Bala_et_al.pdf |
| _version_ | 1848777674128883712 |
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| author | Bala, Muntari Salleh, Hamzah Mohd. Amid, Azura Mel, Maizirwan Jami, Mohammed Saedi |
| author_facet | Bala, Muntari Salleh, Hamzah Mohd. Amid, Azura Mel, Maizirwan Jami, Mohammed Saedi |
| author_sort | Bala, Muntari |
| building | IIUM Repository |
| collection | Online Access |
| description | In spite of the fact that commercial bromelain supplements are available in the market, to date, none of them are produced and formulated from recombinant forms. They are extracted and purified (often partially) from the stem and fruit of pineapple (Ananas comosus). This makes the production of bromelain very difficult, less reliable, often contaminated and expensive. In this study, a recombinant
bromelain was expressed as soluble (active) and insoluble (inactive) enzyme in Escherichia coli BL21-A1. The enzyme fractions were purified using Ni-NTA His•Bind resin with the aid of an automated fast protein liquid chromatography (FPLC) system. Purification fold and percentage yield of the purified active bromelain were found to be 3.7 and 64%, respectively. SDS-PAGE results showed that the enzyme was purified to near homogeneity and the soluble form exhibited a single band with molecular weight of about 45 kDa. |
| first_indexed | 2025-11-14T14:49:44Z |
| format | Article |
| id | iium-14293 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:49:44Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-142932021-07-06T02:10:14Z http://irep.iium.edu.my/14293/ Recovery of recombinant bromelain from Escherichia coli BL21-AI Bala, Muntari Salleh, Hamzah Mohd. Amid, Azura Mel, Maizirwan Jami, Mohammed Saedi T Technology (General) In spite of the fact that commercial bromelain supplements are available in the market, to date, none of them are produced and formulated from recombinant forms. They are extracted and purified (often partially) from the stem and fruit of pineapple (Ananas comosus). This makes the production of bromelain very difficult, less reliable, often contaminated and expensive. In this study, a recombinant bromelain was expressed as soluble (active) and insoluble (inactive) enzyme in Escherichia coli BL21-A1. The enzyme fractions were purified using Ni-NTA His•Bind resin with the aid of an automated fast protein liquid chromatography (FPLC) system. Purification fold and percentage yield of the purified active bromelain were found to be 3.7 and 64%, respectively. SDS-PAGE results showed that the enzyme was purified to near homogeneity and the soluble form exhibited a single band with molecular weight of about 45 kDa. Academic Journals 2011-12-16 Article PeerReviewed application/pdf en http://irep.iium.edu.my/14293/1/Bala_et_al.pdf Bala, Muntari and Salleh, Hamzah Mohd. and Amid, Azura and Mel, Maizirwan and Jami, Mohammed Saedi (2011) Recovery of recombinant bromelain from Escherichia coli BL21-AI. African Journal of Biotechnology, 10 (81). 18829-18832. ISSN 1684–5315 http://www.academicjournals.org/AJB/PDF/pdf2011/16DecConf/Bala%20et%20al.pdf 10.5897/AJB11.2761 |
| spellingShingle | T Technology (General) Bala, Muntari Salleh, Hamzah Mohd. Amid, Azura Mel, Maizirwan Jami, Mohammed Saedi Recovery of recombinant bromelain from Escherichia coli BL21-AI |
| title | Recovery of recombinant bromelain from Escherichia
coli BL21-AI |
| title_full | Recovery of recombinant bromelain from Escherichia
coli BL21-AI |
| title_fullStr | Recovery of recombinant bromelain from Escherichia
coli BL21-AI |
| title_full_unstemmed | Recovery of recombinant bromelain from Escherichia
coli BL21-AI |
| title_short | Recovery of recombinant bromelain from Escherichia
coli BL21-AI |
| title_sort | recovery of recombinant bromelain from escherichia
coli bl21-ai |
| topic | T Technology (General) |
| url | http://irep.iium.edu.my/14293/ http://irep.iium.edu.my/14293/ http://irep.iium.edu.my/14293/ http://irep.iium.edu.my/14293/1/Bala_et_al.pdf |