Enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme

The rapid development and the economic growth of oil palm industry have exacerbated environmental pollution since it results in large quantity of waste products produced from the oil extraction process. In many cases, however, low catalytic efficiency and instability of enzymes were considered as ba...

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Main Authors: Al-Khatib, Ma'an Fahmi Rashid, Mohammed Abd, Rasha, Alam, Md Zahangir, Mohd. Salleh, Hamzah
Format: Article
Language:English
Published: Academic Journals 2011
Subjects:
Online Access:http://irep.iium.edu.my/14274/
http://irep.iium.edu.my/14274/1/Alkhatib_et_al_1.pdf
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author Al-Khatib, Ma'an Fahmi Rashid
Mohammed Abd, Rasha
Alam, Md Zahangir
Mohd. Salleh, Hamzah
author_facet Al-Khatib, Ma'an Fahmi Rashid
Mohammed Abd, Rasha
Alam, Md Zahangir
Mohd. Salleh, Hamzah
author_sort Al-Khatib, Ma'an Fahmi Rashid
building IIUM Repository
collection Online Access
description The rapid development and the economic growth of oil palm industry have exacerbated environmental pollution since it results in large quantity of waste products produced from the oil extraction process. In many cases, however, low catalytic efficiency and instability of enzymes were considered as barriers for the development of large-scale operations and applications. In this study, hydrolysis of oil palm empty fruit bunch to reducing sugar was achieved using cellulase enzyme immobilized on multi-wall carbon nanotubes. The hydrolysis evaluation was performed using free cellulase enzyme. The amount of reducing sugar concentration released during the reaction was determined using dinitrosalicylic acid(DNS) assay. Using the immobilized form of the enzyme, it was found that the reducing sugar concentration was increased and the enzymatic hydrolysis of oil palm empty fruit bunch was enhanced greatly over long time of hydrolysis.
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institution International Islamic University Malaysia
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language English
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publishDate 2011
publisher Academic Journals
recordtype eprints
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spelling iium-142742024-05-02T08:16:14Z http://irep.iium.edu.my/14274/ Enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme Al-Khatib, Ma'an Fahmi Rashid Mohammed Abd, Rasha Alam, Md Zahangir Mohd. Salleh, Hamzah TS Manufactures The rapid development and the economic growth of oil palm industry have exacerbated environmental pollution since it results in large quantity of waste products produced from the oil extraction process. In many cases, however, low catalytic efficiency and instability of enzymes were considered as barriers for the development of large-scale operations and applications. In this study, hydrolysis of oil palm empty fruit bunch to reducing sugar was achieved using cellulase enzyme immobilized on multi-wall carbon nanotubes. The hydrolysis evaluation was performed using free cellulase enzyme. The amount of reducing sugar concentration released during the reaction was determined using dinitrosalicylic acid(DNS) assay. Using the immobilized form of the enzyme, it was found that the reducing sugar concentration was increased and the enzymatic hydrolysis of oil palm empty fruit bunch was enhanced greatly over long time of hydrolysis. Academic Journals 2011-12-16 Article PeerReviewed application/pdf en http://irep.iium.edu.my/14274/1/Alkhatib_et_al_1.pdf Al-Khatib, Ma'an Fahmi Rashid and Mohammed Abd, Rasha and Alam, Md Zahangir and Mohd. Salleh, Hamzah (2011) Enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme. African Journal of Biotechnology, 10 (81). pp. 18811-18815. ISSN 1684–5315 http://www.academicjournals.org/ajb/PDF/pdf2011/16DecConf/Alkhatib%20et%20al%201.pdf 10.5897/AJB11.2758
spellingShingle TS Manufactures
Al-Khatib, Ma'an Fahmi Rashid
Mohammed Abd, Rasha
Alam, Md Zahangir
Mohd. Salleh, Hamzah
Enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme
title Enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme
title_full Enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme
title_fullStr Enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme
title_full_unstemmed Enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme
title_short Enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme
title_sort enzymatic hydrolysis of oil palm empty fruit bunch using immobilized cellulase enzyme
topic TS Manufactures
url http://irep.iium.edu.my/14274/
http://irep.iium.edu.my/14274/
http://irep.iium.edu.my/14274/
http://irep.iium.edu.my/14274/1/Alkhatib_et_al_1.pdf