Purification and characterization of a bacterial phytase whose properties make it exceptionally useful as a feed supplement
A periplasmatic phytase from a bacterium isolated from Malaysian waste water was purified about 173-fold to apparent homogeneity with a recovery of 10% referred to the phytase activity in the crude extract. It behaved as a monomeric protein with a molecular mass of about 42 kDa. The purified enzy...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer
2007
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| Online Access: | http://irep.iium.edu.my/14233/ http://irep.iium.edu.my/14233/1/Purification_and_Characterization.pdf |
| _version_ | 1848777668601839616 |
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| author | Greiner, Ralf Farouk, Abd Elaziem Aziem |
| author_facet | Greiner, Ralf Farouk, Abd Elaziem Aziem |
| author_sort | Greiner, Ralf |
| building | IIUM Repository |
| collection | Online Access |
| description | A periplasmatic phytase from a bacterium isolated from Malaysian waste water was purified
about 173-fold to apparent homogeneity with a recovery of 10% referred to the phytase
activity in the crude extract. It behaved as a monomeric protein with a molecular mass of
about 42 kDa. The purified enzyme exhibited a single pH optimum at 4.5. Optimum
temperature for the degradation of phytate was 65�C. The kinetic parameters for the
hydrolysis of sodium phytate were determined to be KM = 0.15 mmol/l and kcat = 1164 s)1
at pH 4.5 and 37�C. The purified enzyme was shown to be highly specific. Among the
phosphorylated compounds tested, phytate was the only one which was significantly
hydrolysed. Some properties such as considerable activity below pH 3.0, thermal stability
and resistance to pepsin make the enzyme attractive for an application as a feed supplement. |
| first_indexed | 2025-11-14T14:49:39Z |
| format | Article |
| id | iium-14233 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:49:39Z |
| publishDate | 2007 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-142332013-06-18T06:50:03Z http://irep.iium.edu.my/14233/ Purification and characterization of a bacterial phytase whose properties make it exceptionally useful as a feed supplement Greiner, Ralf Farouk, Abd Elaziem Aziem TA Engineering (General). Civil engineering (General) A periplasmatic phytase from a bacterium isolated from Malaysian waste water was purified about 173-fold to apparent homogeneity with a recovery of 10% referred to the phytase activity in the crude extract. It behaved as a monomeric protein with a molecular mass of about 42 kDa. The purified enzyme exhibited a single pH optimum at 4.5. Optimum temperature for the degradation of phytate was 65�C. The kinetic parameters for the hydrolysis of sodium phytate were determined to be KM = 0.15 mmol/l and kcat = 1164 s)1 at pH 4.5 and 37�C. The purified enzyme was shown to be highly specific. Among the phosphorylated compounds tested, phytate was the only one which was significantly hydrolysed. Some properties such as considerable activity below pH 3.0, thermal stability and resistance to pepsin make the enzyme attractive for an application as a feed supplement. Springer 2007-10 Article PeerReviewed application/pdf en http://irep.iium.edu.my/14233/1/Purification_and_Characterization.pdf Greiner, Ralf and Farouk, Abd Elaziem Aziem (2007) Purification and characterization of a bacterial phytase whose properties make it exceptionally useful as a feed supplement. Protein Journal, 26 (7). pp. 467-474. ISSN 1572-3887 http://www.springerlink.com/content/d347721083j44772/ |
| spellingShingle | TA Engineering (General). Civil engineering (General) Greiner, Ralf Farouk, Abd Elaziem Aziem Purification and characterization of a bacterial phytase whose properties make it exceptionally useful as a feed supplement |
| title | Purification and characterization of a bacterial phytase
whose properties make it exceptionally useful
as a feed supplement |
| title_full | Purification and characterization of a bacterial phytase
whose properties make it exceptionally useful
as a feed supplement |
| title_fullStr | Purification and characterization of a bacterial phytase
whose properties make it exceptionally useful
as a feed supplement |
| title_full_unstemmed | Purification and characterization of a bacterial phytase
whose properties make it exceptionally useful
as a feed supplement |
| title_short | Purification and characterization of a bacterial phytase
whose properties make it exceptionally useful
as a feed supplement |
| title_sort | purification and characterization of a bacterial phytase
whose properties make it exceptionally useful
as a feed supplement |
| topic | TA Engineering (General). Civil engineering (General) |
| url | http://irep.iium.edu.my/14233/ http://irep.iium.edu.my/14233/ http://irep.iium.edu.my/14233/1/Purification_and_Characterization.pdf |