Fabrication of ceramic membrane chromatography for biologics purification
Chromatography is one of the most important separation processes of choice for the recovery/purification of proteins and complex bio-structures. Fabrication of chromatographic membranes and their efficiency in the chromatography process has been the subject of many recent researches. In this stud...
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| Format: | Article |
| Language: | English |
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IIUM Press
2011
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| Online Access: | http://irep.iium.edu.my/13933/ http://irep.iium.edu.my/13933/1/209_Ceramic_membrane.pdf |
| _version_ | 1848777630792286208 |
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| author | Mel, Maizirwan Abdeen, Fouad R. H. Sopyan, Iis |
| author_facet | Mel, Maizirwan Abdeen, Fouad R. H. Sopyan, Iis |
| author_sort | Mel, Maizirwan |
| building | IIUM Repository |
| collection | Online Access |
| description | Chromatography is one of the most important separation processes of
choice for the recovery/purification of proteins and complex bio-structures. Fabrication
of chromatographic membranes and their efficiency in the chromatography process has
been the subject of many recent researches. In this study, a coin-like, 13 mm diameter
and 3 mm thick, ceramic membrane was fabricated to be used as a chromatographic
medium. The membrane is used to replace the conventional resin-based chromatography
columns. Hydroxyapatite (HA) powder was used as a material for the membrane
fabrication. In this project, a HA powder was produced using starch as pore creating
agents. Characterization processes were done for the ceramic membrane using the
suitable apparatuses. Three parameters of the fabrication process (starch wt %,
compaction pressure and sintering temperature) were manipulated to optimize the
performance of the membrane. The fabricated membrane was placed in a (FPLC)
system to be tested for its performance as an adsorptive membrane. (IMAC) process was
run by immobilizing Ni2+ ions at the membrane particles surfaces. NP protein of the
(NDV) was used to test the membrane’s ability to bind Histidine-tagged proteins. The
optimum set of process parameters that yielded in the highest porosity and good
chromatogram was determined to be 5 wt % starch, 3000 psi compaction pressure and
1100°C sintering temperature. |
| first_indexed | 2025-11-14T14:49:03Z |
| format | Article |
| id | iium-13933 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:49:03Z |
| publishDate | 2011 |
| publisher | IIUM Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-139332012-01-04T06:42:36Z http://irep.iium.edu.my/13933/ Fabrication of ceramic membrane chromatography for biologics purification Mel, Maizirwan Abdeen, Fouad R. H. Sopyan, Iis TP155 Chemical engineering TP248.13 Biotechnology Chromatography is one of the most important separation processes of choice for the recovery/purification of proteins and complex bio-structures. Fabrication of chromatographic membranes and their efficiency in the chromatography process has been the subject of many recent researches. In this study, a coin-like, 13 mm diameter and 3 mm thick, ceramic membrane was fabricated to be used as a chromatographic medium. The membrane is used to replace the conventional resin-based chromatography columns. Hydroxyapatite (HA) powder was used as a material for the membrane fabrication. In this project, a HA powder was produced using starch as pore creating agents. Characterization processes were done for the ceramic membrane using the suitable apparatuses. Three parameters of the fabrication process (starch wt %, compaction pressure and sintering temperature) were manipulated to optimize the performance of the membrane. The fabricated membrane was placed in a (FPLC) system to be tested for its performance as an adsorptive membrane. (IMAC) process was run by immobilizing Ni2+ ions at the membrane particles surfaces. NP protein of the (NDV) was used to test the membrane’s ability to bind Histidine-tagged proteins. The optimum set of process parameters that yielded in the highest porosity and good chromatogram was determined to be 5 wt % starch, 3000 psi compaction pressure and 1100°C sintering temperature. IIUM Press 2011-12-28 Article PeerReviewed application/pdf en http://irep.iium.edu.my/13933/1/209_Ceramic_membrane.pdf Mel, Maizirwan and Abdeen, Fouad R. H. and Sopyan, Iis (2011) Fabrication of ceramic membrane chromatography for biologics purification. IIUM Engineering Journal, 12 (4). pp. 115-124. ISSN 1511-788X http://www.iium.edu.my/ejournal/index.php/iiumej/article/viewArticle/213 |
| spellingShingle | TP155 Chemical engineering TP248.13 Biotechnology Mel, Maizirwan Abdeen, Fouad R. H. Sopyan, Iis Fabrication of ceramic membrane chromatography for biologics purification |
| title | Fabrication of ceramic membrane chromatography for biologics purification |
| title_full | Fabrication of ceramic membrane chromatography for biologics purification |
| title_fullStr | Fabrication of ceramic membrane chromatography for biologics purification |
| title_full_unstemmed | Fabrication of ceramic membrane chromatography for biologics purification |
| title_short | Fabrication of ceramic membrane chromatography for biologics purification |
| title_sort | fabrication of ceramic membrane chromatography for biologics purification |
| topic | TP155 Chemical engineering TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/13933/ http://irep.iium.edu.my/13933/ http://irep.iium.edu.my/13933/1/209_Ceramic_membrane.pdf |