Quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases
Diabetes mellitus causes several changes in fiber size and oxidative capacity in skeletal muscle. Gastrocnemius, Soleus and Anterior Tibialis muscle are important leg muscles involve in reconstruction of amputation stump. The fiber size and oxidative enzyme are important factors related to the viabi...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/13782/ http://irep.iium.edu.my/13782/1/Histological_%26_Histochemical_studies_of_LL_muscles_in_Diabetis_Mellitus_cases.pdf |
| _version_ | 1848777609124511744 |
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| author | W. S. , Wan Muhammad Salahudin Tun, Ye Zulkifly, Ahmad Hafiz |
| author_facet | W. S. , Wan Muhammad Salahudin Tun, Ye Zulkifly, Ahmad Hafiz |
| author_sort | W. S. , Wan Muhammad Salahudin |
| building | IIUM Repository |
| collection | Online Access |
| description | Diabetes mellitus causes several changes in fiber size and oxidative capacity in skeletal muscle. Gastrocnemius, Soleus and Anterior Tibialis muscle are important leg muscles involve in reconstruction of amputation stump. The fiber size and oxidative enzyme are important factors related to the viability of muscle and subsequently stump healing.
Aim of the study: To describe the fiber size and oxidative capacity quantitatively in 3 muscles of the leg
Materials and methods: Specimen obtained from the 10 diabetes mellitus subjects undergone below knee amputation (BKA). Specimen was stained for enzyme histochemistry NADH-TR staining. The slide was analyzed with ImageJ software for fiber type distribution and oxidative capacity based on staining density.
Result and discussion: Gastrocnemius muscle has predominantly Type 1 muscle fibers and highest oxidative capacity, while the anterior tibialis muscle has predominantly Type 2 muscle fibers and lowest oxidative capacity. Soleus muscle has almost equal Type 1 and 2 muscle fibers and has intermediate oxidative capacity.
Conclusion: Muscle with predominant type 1 fibers has highest oxidative capacity. |
| first_indexed | 2025-11-14T14:48:42Z |
| format | Proceeding Paper |
| id | iium-13782 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:48:42Z |
| publishDate | 2011 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-137822017-06-21T06:55:26Z http://irep.iium.edu.my/13782/ Quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases W. S. , Wan Muhammad Salahudin Tun, Ye Zulkifly, Ahmad Hafiz QM Human anatomy Diabetes mellitus causes several changes in fiber size and oxidative capacity in skeletal muscle. Gastrocnemius, Soleus and Anterior Tibialis muscle are important leg muscles involve in reconstruction of amputation stump. The fiber size and oxidative enzyme are important factors related to the viability of muscle and subsequently stump healing. Aim of the study: To describe the fiber size and oxidative capacity quantitatively in 3 muscles of the leg Materials and methods: Specimen obtained from the 10 diabetes mellitus subjects undergone below knee amputation (BKA). Specimen was stained for enzyme histochemistry NADH-TR staining. The slide was analyzed with ImageJ software for fiber type distribution and oxidative capacity based on staining density. Result and discussion: Gastrocnemius muscle has predominantly Type 1 muscle fibers and highest oxidative capacity, while the anterior tibialis muscle has predominantly Type 2 muscle fibers and lowest oxidative capacity. Soleus muscle has almost equal Type 1 and 2 muscle fibers and has intermediate oxidative capacity. Conclusion: Muscle with predominant type 1 fibers has highest oxidative capacity. 2011-12-21 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/13782/1/Histological_%26_Histochemical_studies_of_LL_muscles_in_Diabetis_Mellitus_cases.pdf W. S. , Wan Muhammad Salahudin and Tun, Ye and Zulkifly, Ahmad Hafiz (2011) Quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases. In: 20th Scientific Conference of the Microscopy Society of Malaysia & 21st Annual General Meeting, 20-22 December 2011, Port Disckson, Negeri Sembilan. (Unpublished) |
| spellingShingle | QM Human anatomy W. S. , Wan Muhammad Salahudin Tun, Ye Zulkifly, Ahmad Hafiz Quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases |
| title | Quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases |
| title_full | Quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases |
| title_fullStr | Quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases |
| title_full_unstemmed | Quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases |
| title_short | Quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases |
| title_sort | quantitative histological and histochemical studies of different muscles of the lower limb in diabetes mellitus cases |
| topic | QM Human anatomy |
| url | http://irep.iium.edu.my/13782/ http://irep.iium.edu.my/13782/1/Histological_%26_Histochemical_studies_of_LL_muscles_in_Diabetis_Mellitus_cases.pdf |